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Microbiol."],"abstract":"<jats:p>\n                    Septic arthritis, most often caused by\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    , is a rapidly progressive and destructive joint disease with substantial mortality and morbidity.\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    lipoproteins (Lpps) are known to induce arthritis and bone destruction. Here, we aimed to investigate the bone resorptive effect of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    Lpps in a murine arthritis model by intra-articular injection of purified\n                    <jats:italic>S. aureus<\/jats:italic>\n                    Lpps, synthetic lipopeptides, and live\n                    <jats:italic>S. aureus<\/jats:italic>\n                    strains. Analyses of the bone mineral density (BMD) of the distal femur bone were performed. Intra-articular injection of both live\n                    <jats:italic>S. aureus<\/jats:italic>\n                    and purified\n                    <jats:italic>S. aureus<\/jats:italic>\n                    Lpps were shown to significantly decrease total- and trabecular BMD. Liquid chromatography\u2013mass spectrometry analyses revealed that the Lpps expressed by\n                    <jats:italic>S. aureus<\/jats:italic>\n                    SA113 strain contain both diacyl and triacyl lipid moieties. Interestingly, synthetic diacylated lipopeptide, Pam\n                    <jats:sub>2<\/jats:sub>\n                    CSK\n                    <jats:sub>4<\/jats:sub>\n                    , was more potent in inducing bone resorption than synthetic triacylated lipopeptide, Pam\n                    <jats:sub>3<\/jats:sub>\n                    CSK\n                    <jats:sub>4<\/jats:sub>\n                    . Modified lipoproteins lacking the lipid moiety were deprived of their bone resorptive abilities. Monocyte depletion by clodronate liposomes fully abrogated the bone resorptive capacity of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    lipoproteins. Our data suggest that\n                    <jats:italic>S. aureus<\/jats:italic>\n                    Lpps induce bone resorption in locally-induced murine arthritis, an effect mediated by their lipid-moiety through monocytes\/macrophages.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2022.843799","type":"journal-article","created":{"date-parts":[[2022,3,9]],"date-time":"2022-03-09T04:04:15Z","timestamp":1646798655000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Lipoproteins Cause Bone Resorption in a Mouse Model of Staphylococcus aureus Septic Arthritis"],"prefix":"10.3389","volume":"13","author":[{"given":"Michelle","family":"Schultz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majd","family":"Mohammad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minh-Thu","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhicheng","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Jarneborn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carina M.","family":"Wienken","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matti","family":"Froning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rille","family":"Pullerits","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abukar","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heiko","family":"Hayen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friedrich","family":"G\u00f6tz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1161","DOI":"10.1002\/eji.1830240523","article-title":"Clonal expansion of T lymphocytes causes arthritis and mortality in mice infected with toxic shock syndrome toxin-1-producing staphylococci","volume":"24","author":"Abdelnour","year":"1994","journal-title":"Eur. 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