{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T12:58:02Z","timestamp":1792155482830,"version":"4.4.0"},"reference-count":59,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T00:00:00Z","timestamp":1646265600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    is the most common pathogen causing osteomyelitis (OM). The aim of this study was to explore the clonal complex (CC) distribution and the pattern of virulence determinants of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    isolates from OM in Italy. Whole-genome sequencing was performed on 83\n                    <jats:italic>S. aureus<\/jats:italic>\n                    isolates from OM cases in six hospitals. Antibiotic susceptibility tests showed that 30.1% of the isolates were methicillin-resistant\n                    <jats:italic>S. aureus<\/jats:italic>\n                    (MRSA). The most frequent CCs detected were CC22, CC5, CC8, CC30, and CC15, which represent the most common lineages circulating in Italian hospitals. MRSA were limited in the number of lineages (CC22, CC5, CC8, and CC1). Phylogenetic analysis followed the sequence type-CC groupings and revealed a non-uniform distribution of the isolates from the different hospitals. No significant difference in the mean number of virulence genes carried by MRSA or MSSA isolates was observed. Some virulence genes, namely\n                    <jats:italic>cna<\/jats:italic>\n                    ,\n                    <jats:italic>fib<\/jats:italic>\n                    ,\n                    <jats:italic>fnbA<\/jats:italic>\n                    ,\n                    <jats:italic>coa<\/jats:italic>\n                    ,\n                    <jats:italic>lukD<\/jats:italic>\n                    ,\n                    <jats:italic>lukE<\/jats:italic>\n                    ,\n                    <jats:italic>sak<\/jats:italic>\n                    , and\n                    <jats:italic>tst<\/jats:italic>\n                    , were correlated with the CC. However, different categories of virulence factors, such as adhesins, exoenzymes, and toxins, were frequently detected and unevenly distributed among all lineages. Indeed, each lineage carried a variable combination of virulence genes, likely reflecting functional redundancy, and arguing for the importance of those traits for the pathogenicity in OM. In conclusion, no specific genetic trait in the most frequent lineages could explain their high prevalence among OM isolates. Our findings highlight that CCs detected in OM isolates follow the epidemiology of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    infections in the country. It is conceivable that any of the most common\n                    <jats:italic>S. aureus<\/jats:italic>\n                    CC can cause a variety of infections, including OM.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2022.846167","type":"journal-article","created":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T01:58:54Z","timestamp":1646272734000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":16,"title":["Virulence Determinants in Staphylococcus aureus Clones Causing Osteomyelitis in Italy"],"prefix":"10.3389","volume":"13","author":[{"given":"Fernanda","family":"Pimentel de Araujo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattia","family":"Pirolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Monaco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Del Grosso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Ambretti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donatella","family":"Lombardo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiziana","family":"Cassetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffaele","family":"Gargiulo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Riccobono","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Visca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annalisa","family":"Pantosti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,3]]},"reference":[{"key":"ref2","doi-asserted-by":"publisher","first-page":"2872","DOI":"10.1128\/IAI.69.5.2872-2877.2001","article-title":"Staphylococcus aureus fibronectin binding proteins are essential for internalization by osteoblasts but do not account for differences in intracellular levels of bacteria","volume":"69","author":"Ahmed","year":"2001","journal-title":"Infect. Immun."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"2590","DOI":"10.1128\/AAC.42.10.2590","article-title":"Characterization of mutations in the rpoB gene that confer rifampin resistance in Staphylococcus aureus","volume":"42","author":"Aubry-Damon","year":"1998","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"1753","DOI":"10.1099\/jmm.0.000859","article-title":"Staphylococcus aureus osteoarticular infections in children: an 8-year review of molecular microbiology, antibiotic resistance and clinical characteristics","volume":"67","author":"Bouras","year":"2018","journal-title":"J. Med. Microbiol."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"1264","DOI":"10.1093\/jac\/dkp107","article-title":"One hundred and twelve infected arthroplasties treated with \u2018DAIR\u2019 (debridement, antibiotics and implant retention): antibiotic duration and outcome","volume":"63","author":"Byren","year":"2009","journal-title":"J. Antimicrob. Chemother."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"4108","DOI":"10.1016\/j.biomaterials.2008.07.006","article-title":"Molecular epidemiology of Staphylococcus aureus from implant orthopaedic infections: Ribotypes, agr polymorphism, leukocidal toxins and antibiotic resistance","volume":"29","author":"Campoccia","year":"2008","journal-title":"Biomaterials"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"1927","DOI":"10.1128\/IAI.00046-11","article-title":"Role of the accessory gene regulator agr in community-associated methicillin-resistant Staphylococcus aureus pathogenesis","volume":"79","author":"Cheung","year":"2011","journal-title":"Infect. Immun."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1016\/j.jhin.2017.12.011","article-title":"Virulence patterns of Staphylococcus aureus strains from nasopharyngeal colonization","volume":"100","author":"Deinhardt-Emmer","year":"2018","journal-title":"J. Hosp. Infect."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1016\/S8756-3282(01)00632-9","article-title":"Staphylococcus aureus collagen adhesin contributes to the pathogenesis of osteomyelitis","volume":"30","author":"Elasri","year":"2002","journal-title":"Bone"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1038\/nrmicro3161","article-title":"Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus","volume":"12","author":"Foster","year":"2014","journal-title":"Nat. Rev. Microbiol."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"24","DOI":"10.3109\/00365548.2011.603744","article-title":"Staphylococcus aureus in a northern Italian region: phenotypic and molecular characterization","volume":"44","author":"Gagliotti","year":"2012","journal-title":"Scand. J. Infect. Dis."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"e0130415","DOI":"10.1371\/journal.pone.0130415","article-title":"Genomic heterogeneity of methicillin resistant Staphylococcus aureus associated with variation in severity of illness among children with acute hematogenous osteomyelitis","volume":"10","author":"Gaviria-Agudelo","year":"2015","journal-title":"PLoS One"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1080\/14737167.2019.1567337","article-title":"Current health and economic burden of chronic diabetic osteomyelitis","volume":"19","author":"Geraghty","year":"2019","journal-title":"Expert Rev. Pharmacoecon. Outcomes Res."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"3373","DOI":"10.1128\/iai.63.9.3373-3380.1995","article-title":"Role of the accessory gene regulator (agr) in pathogenesis of staphylococcal osteomyelitis","volume":"63","author":"Gillaspy","year":"1995","journal-title":"Infect. Immun."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"961","DOI":"10.1016\/j.cmi.2017.04.006","article-title":"Colonization by multidrug-resistant organisms in long-term care facilities in Italy: a point-prevalence study","volume":"23","author":"Giufr\u00e8","year":"2017","journal-title":"Clin. Microbiol. Infect."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1017\/S0950268807008850","article-title":"Epidemiology of vertebral osteomyelitis (VO) in France: analysis of hospital-discharge data 2002-2003","volume":"136","author":"Grammatico","year":"2008","journal-title":"Epidemiol. Infect."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"e00859","DOI":"10.1128\/IAI.00859-19","article-title":"Staphylococcus aureus fibronectin binding protein A mediates biofilm development and infection","volume":"88","author":"Gries","year":"2020","journal-title":"Infect. Immun."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"20987","DOI":"10.2807\/1560-7917.ES2014.19.49.20987","article-title":"The dynamic changes of dominant clones of Staphylococcus aureus causing bloodstream infections in the European region: results of a second structured survey","volume":"19","author":"Grundmann","year":"2014","journal-title":"Eur. Secur."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"1083","DOI":"10.1111\/j.1365-2958.1996.tb02548.x","article-title":"Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis","volume":"20","author":"Heilmann","year":"1996","journal-title":"Mol. Microbiol."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"760","DOI":"10.1111\/jpc.12251","article-title":"Systematic review of duration and choice of systemic antibiotic therapy for acute haematogenous bacterial osteomyelitis in children","volume":"49","author":"Howard-Jones","year":"2013","journal-title":"J. Paediatr. Child Health"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/microbiolspec.GPP3-0031-2018","article-title":"Regulation of Staphylococcus aureus virulence","volume":"7","author":"Jenul","year":"2019","journal-title":"Microbiol. Spectr."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"D566","DOI":"10.1093\/nar\/gkw1004","article-title":"CARD 2017: expansion and model-centric curation of the comprehensive antibiotic resistance database","volume":"45","author":"Jia","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"1021","DOI":"10.1097\/00006454-199811000-00012","article-title":"Epidemiology and outcome of osteomyelitis in the era of sequential intravenous-oral therapy","volume":"17","author":"Karwowska","year":"1998","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1111\/j.1574-695X.2011.00821.x","article-title":"Quantitative analysis of biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA) strains from patients with orthopaedic device-related infections","volume":"63","author":"Kawamura","year":"2011","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"1020","DOI":"10.1111\/j.1469-0691.2010.03367.x","article-title":"Molecular analysis of community-acquired methicillin-susceptible and resistant Staphylococcus aureus isolates recovered from bacteraemic and osteomyelitis infections in children from Tunisia","volume":"17","author":"Kechrid","year":"2011","journal-title":"Clin. Microbiol. Infect."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"837","DOI":"10.2106\/JBJS.N.01350","article-title":"Trends in the epidemiology of osteomyelitis: a population-based study, 1969 to 2009","volume":"97","author":"Kremers","year":"2015","journal-title":"J. Bone Joint Surg. Am."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.arth.2012.02.022","article-title":"Economic burden of periprosthetic joint infection in the United States","volume":"27","author":"Kurtz","year":"2012","journal-title":"J. Arthroplast."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"2365","DOI":"10.1128\/JCM.00262-14","article-title":"Bacterial whole-genome sequencing revisited: portable, scalable, and standardized analysis for typing and detection of virulence and antibiotic resistance genes","volume":"52","author":"Leopold","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"669","DOI":"10.1128\/JB.188.2.669-676.2006","article-title":"Microarrays reveal that each of the ten dominant lineages of Staphylococcus aureus has a unique combination of surface-associated and regulatory genes","volume":"188","author":"Lindsay","year":"2006","journal-title":"J. Bacteriol."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1007\/s10096-010-0884-4","article-title":"Molecular fingerprinting of Staphylococcus aureus from bone and joint infections","volume":"29","author":"Luedicke","year":"2010","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1007\/82_2016_497","article-title":"Epidemiology of Staphylococcus aureus nasal carriage patterns in the community","volume":"398","author":"Mehraj","year":"2016","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"1945","DOI":"10.1016\/s0142-9612(99)00099-x","article-title":"Presence and expression of collagen adhesin gene (cna) and slime production in Staphylococcus aureus strains from orthopaedic prosthesis infections","volume":"20","author":"Montanaro","year":"1999","journal-title":"Biomaterials"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"8","DOI":"10.3389\/fcimb.2016.00008","article-title":"Molecular characterization of a prevalent ribocluster of methicillin-sensitive Staphylococcus aureus from orthopedic implant infections. Correspondence with MLST CC30","volume":"6","author":"Montanaro","year":"2016","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1002\/jbm.a.32764","article-title":"Polymorphisms of agr locus correspond to distinct genetic patterns of virulence in Staphylococcus aureus clinical isolates from orthopedic implant infections","volume":"94","author":"Montanaro","year":"2010","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"1443","DOI":"10.1007\/s10096-015-2371-4","article-title":"Impacts of enterotoxin gene cluster-encoded superantigens on local and systemic experimental Staphylococcus aureus infections","volume":"34","author":"Nowrouzian","year":"2015","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1016\/j.ijmm.2013.02.007","article-title":"Community-associated MRSA: what makes them special?","volume":"303","author":"Otto","year":"2013","journal-title":"Int. J. Med. Microbiol."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"791","DOI":"10.1086\/427663","article-title":"Fibronectin-binding proteins and fibrinogen-binding clumping factors play distinct roles in staphylococcal arthritis and systemic inflammation","volume":"191","author":"Palmqvist","year":"2005","journal-title":"J. Infect. Dis."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1128\/iai.62.1.152-161.1994","article-title":"The Staphylococcus aureus collagen adhesin is a virulence determinant in experimental septic arthritis","volume":"62","author":"Patti","year":"1994","journal-title":"Infect. Immun."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"4987","DOI":"10.1128\/IAI.70.9.4987-4996.2002","article-title":"Virulent combinations of adhesin and toxin genes in natural populations of Staphylococcus aureus","volume":"70","author":"Peacock","year":"2002","journal-title":"Infect. Immun."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.jgar.2021.03.030","article-title":"Staphylococcus aureus clones causing osteomyelitis: a literature review (2000\u20132020)","volume":"26","author":"Pimentel de Araujo","year":"2021","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1016\/j.ijmm.2014.03.003","article-title":"Phenotypic and genotypic characterisation of Staphylococcus aureus causing musculoskeletal infections","volume":"304","author":"Post","year":"2014","journal-title":"Int. J. Med. Microbiol."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"e77477","DOI":"10.1371\/journal.pone.0077477","article-title":"Prevalence of clonal complexes and virulence genes among commensal and invasive Staphylococcus aureus isolates in Sweden","volume":"8","author":"Rasmussen","year":"2013","journal-title":"PLoS One"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1007\/s12178-018-9501-4","article-title":"Staphylococcus aureus evasion of host immunity in the setting of prosthetic joint infection: biofilm and beyond","volume":"11","author":"Ricciardi","year":"2018","journal-title":"Curr. Rev. Musculoskelet. Med."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"e71259","DOI":"10.1371\/journal.pone.0071259","article-title":"Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study","volume":"8","author":"Rieg","year":"2013","journal-title":"PLoS One"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"O274","DOI":"10.1111\/1469-0691.12375","article-title":"First report of the predominance of clonal complex 398 Staphylococcus aureus strains in osteomyelitis complicating diabetic foot ulcers: a national French study","volume":"20","author":"Senneville","year":"2014","journal-title":"Clin. Microbiol. Infect."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"102176","DOI":"10.1016\/j.amsu.2021.02.022","article-title":"Retrospective analysis of trends in surgery volumes between 2016 and 2019 and impact of the insurance deductible: cross-sectional study","volume":"63","author":"Shukla","year":"2021","journal-title":"Ann. Med. Surg."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"1008","DOI":"10.1128\/JCM.03022-15","article-title":"Detecting Staphylococcus aureus virulence and resistance genes: a comparison of whole-genome sequencing and DNA microarray technology","volume":"54","author":"Strau\u00df","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"ref51","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1007\/s101560070010","article-title":"Mechanism of quinolone resistance in Staphylococcus aureus","volume":"6","author":"Tanaka","year":"2000","journal-title":"J. Infect. Chemother."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"e0200064","DOI":"10.1371\/journal.pone.0200064","article-title":"Association between biofilm formation phenotype and clonal lineage in Staphylococcus aureus strains from bone and joint infections","volume":"13","author":"Tasse","year":"2018","journal-title":"PLoS One"},{"key":"ref53","doi-asserted-by":"publisher","first-page":"789","DOI":"10.5301\/ijao.5000058","article-title":"Internalization by osteoblasts of two Staphylococcus aureus clinical isolates differing in their adhesin gene pattern","volume":"34","author":"Testoni","year":"2011","journal-title":"Int. J. Artif. Organs"},{"key":"ref54","doi-asserted-by":"publisher","first-page":"394","DOI":"10.1007\/s11908-008-0064-1","article-title":"Diagnosis and treatment of implant-associated septic arthritis and osteomyelitis","volume":"10","author":"Trampuz","year":"2008","journal-title":"Curr. Infect. Dis. Rep."},{"key":"ref55","doi-asserted-by":"publisher","first-page":"135","DOI":"10.3390\/toxins11030135","article-title":"Clinical S. aureus isolates vary in their virulence to promote adaptation to the host","volume":"11","author":"Tuchscherr","year":"2019","journal-title":"Toxins"},{"key":"ref56","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1042\/bj3450611","article-title":"A bone sialoprotein-binding protein from Staphylococcus aureus: a member of the staphylococcal Sdr family","volume":"345","author":"Tung","year":"2000","journal-title":"Biochem. J."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"O772","DOI":"10.1111\/1469-0691.12567","article-title":"Methicillin-susceptible Staphylococcus aureus clonal complex 398: high prevalence and geographical heterogeneity in bone and joint infection and nasal carriage","volume":"20","author":"Valour","year":"2014","journal-title":"Clin. Microbiol. Infect."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.meegid.2008.09.012","article-title":"Co-evolutionary aspects of human colonisation and infection by Staphylococcus aureus","volume":"9","author":"van Belkum","year":"2009","journal-title":"Infect. Genet. Evol."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"1569","DOI":"10.1128\/JCM.40.4.1569-1570.2002","article-title":"Detection of Staphylococcus aureus clinical isolates harboring the Ica gene cluster needed for biofilm establishment","volume":"40","author":"VictoriaMart\u00edn-L\u00f3pez","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref60","doi-asserted-by":"publisher","first-page":"5938","DOI":"10.1038\/s41598-020-62751-z","article-title":"Genomic characterization and outcome of prosthetic joint infections caused by Staphylococcus aureus","volume":"10","author":"Wildeman","year":"2020","journal-title":"Sci. Rep."},{"key":"ref61","doi-asserted-by":"publisher","first-page":"2640","DOI":"10.1093\/jac\/dks261","article-title":"Identification of acquired antimicrobial resistance genes","volume":"67","author":"Zankari","year":"2012","journal-title":"J. Antimicrob. 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