{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:18:28Z","timestamp":1787026708581,"version":"build-2736575974"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T00:00:00Z","timestamp":1647993600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from\n                    <jats:italic>Flammulina velutipes<\/jats:italic>\n                    (FVPs). The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology. Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content. The anti-inflammatory ability of the FVPs is reflected in a decrease in pro-inflammatory cytokines (including IL-6, IL-1\u03b2, and TNF-\u03b1). 16S rRNA sequencing shows that the FVPs change the composition of the gut microbiota. A subsequent metabolomics analysis of the gut bacteria (UHPLC\u2013MS\/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect. This study provides a potential way to modulate gut microbiota and manage liver diseases using natural products.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2022.847653","type":"journal-article","created":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T19:08:12Z","timestamp":1648494492000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Flammulinavelutipes Polysaccharides Modulate Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice"],"prefix":"10.3389","volume":"13","author":[{"given":"Yingyin","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihong","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,23]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0009-8981(01)00393-X","article-title":"Methods for monitoring oxidative stress, lipid peroxidation and oxidation resistance of lipoproteins","volume":"306","author":"Abuja","year":"2001","journal-title":"Clin. 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Polym."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.foodchem.2017.03.161","article-title":"Comparison of flavour qualities of mushrooms (Flammulina velutipes) packed with different packaging materials","volume":"232","author":"Fang","year":"2017","journal-title":"Food Chem."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.jare.2020.01.001","article-title":"Metabolomics reveals impact of seven functional foods on metabolic pathways in a gut microbiota model","volume":"23","author":"Farag","year":"2020","journal-title":"J. Adv. Res."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"83","DOI":"10.3892\/mmr.2014.2735","article-title":"Octreotide attenuates liver fibrosis by inhibiting hepatic heme oxygenase-1 expression","volume":"11","author":"Guo","year":"2015","journal-title":"Mol. Med. 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Res."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"1099","DOI":"10.1016\/j.celrep.2018.03.109","article-title":"Gut microbiota-derived tryptophan metabolites modulate inflammatory response in hepatocytes and macrophages","volume":"23","author":"Krishnan","year":"2018","journal-title":"Cell Rep."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1186\/s40168-018-0498-0","article-title":"Metabolomic signatures distinguish the impact of formula carbohydrates on disease outcome in a preterm piglet model of NEC","volume":"6","author":"Lee","year":"2018","journal-title":"Microbiome"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.fct.2014.06.011","article-title":"Glutathione S-transferase A1 (GSTA1) release, an early indicator of acute hepatic injury in mice","volume":"71","author":"Liu","year":"2014","journal-title":"Food Chem. 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Acta"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"1457","DOI":"10.3390\/nu10101457","article-title":"Gut microbiota's relationship with liver disease and role in hepatoprotection by dietary natural products and probiotics","volume":"10","author":"Meng","year":"2018","journal-title":"Nutrients"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1016\/S0301-0082(00)00015-0","article-title":"The role of oxidative stress in the toxicity induced by amyloid \u03b2-peptide in Alzheimer's disease","volume":"62","author":"Miranda","year":"2000","journal-title":"Prog. Neurobiol."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1016\/j.carbpol.2007.04.010","article-title":"Purification, characterization and biological activity on hepatocytes of a polysaccharide from Flammulina velutipes mycelium","volume":"70","author":"Pang","year":"2007","journal-title":"Carbohydr. Polym."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.jchromb.2014.01.044","article-title":"Metabonomic study of Wu-tou decoction in adjuvant-induced arthritis rat using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry","author":"Qi","year":"2014","journal-title":"J. Chromatogr. B"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"1046","DOI":"10.1007\/s12026-017-8946-7","article-title":"Von Willebrand factor protects against acute CCl4-induced hepatotoxicity through phospho-p38 MAPK signaling pathway inhibition","volume":"65","author":"Sun","year":"2017","journal-title":"Immunol. Res."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1038\/nature05414","article-title":"An obesity-associated gut microbiome with increased capacity for energy harvest","volume":"444","author":"Turnbaugh","year":"2006","journal-title":"Nature"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1016\/j.biocel.2006.07.001","article-title":"Free radicals and antioxidants in normal physiological functions and human disease","volume":"39","author":"Valko","year":"2007","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"788","DOI":"10.1016\/j.ijbiomac.2018.11.260","article-title":"Characterizations and hepatoprotective effect of polysaccharides from mesona blumes against tetrachloride-induced acute liver injury in mice","volume":"124","author":"Wang","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"536","DOI":"10.1016\/j.fshw.2021.04.016","article-title":"Antioxidative and hepatoprotective activities of a novel polysaccharide (LSAP) from Lepista sordida mycelia","volume":"10","author":"Xu","year":"","journal-title":"Food Sci. Human Wellness"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"648162","DOI":"10.3389\/fimmu.2021.648162","article-title":"Tremella fuciformis polysaccharides inhibited colonic inflammation in dextran sulfate sodium-treated mice via Foxp3+ T cells, gut microbiota, and bacterial metabolites","volume":"12","author":"Xu","year":"","journal-title":"Front. Immunol."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"12250","DOI":"10.3390\/molecules200712250","article-title":"Protective effect of procyanidin B2 against CCl4-induced acute liver injury in mice","volume":"20","author":"Yang","year":"2015","journal-title":"Molecules"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1002\/hep.29327","article-title":"The liver-gut microbiota axis modulates hepatotoxicity of tacrine in the rat","volume":"67","author":"Yip","year":"2018","journal-title":"Hepatology"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1016\/j.ijbiomac.2017.12.149","article-title":"Metabonomic profiling in study hepatoprotective effect of polysaccharides from Flammulina velutipes on carbon tetrachloride-induced acute liver injury rats using GC-MS","volume":"110","author":"Zhang","year":"2018","journal-title":"Int. J. Biol. 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