{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T12:03:39Z","timestamp":1781093019706,"version":"3.54.1"},"reference-count":35,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,4,28]],"date-time":"2022-04-28T00:00:00Z","timestamp":1651104000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973382"],"award-info":[{"award-number":["81973382"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960381"],"award-info":[{"award-number":["81960381"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700011"],"award-info":[{"award-number":["81700011"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p><jats:italic>Pseudomonas aeruginosa<\/jats:italic> can cause infections in the blood, lungs (pneumonia), or other parts of the body after surgery. To investigate the molecular characteristics of \u03b2-lactam antibiotic resistance of <jats:italic>P. aeruginosa<\/jats:italic> isolated from a hospital population between 2015 and 2017, in this study, the antimicrobial susceptibility and the resistance gene profile of the bacteria were determined. The Pulsed-field gel electrophoresis (PFGE) was used to characterize the clonal relatedness and sequencing and comparative genomic analysis were performed to analyze the structure of the resistance gene-related sequences. As a result, of the 260 <jats:italic>P<\/jats:italic>. <jats:italic>aeruginosa<\/jats:italic> strains analyzed, the resistance rates for 6 \u03b2-lactam antibiotics ranged from 4.6 to 9.6%. A total of 7 genotypes of 44 \u03b2-lactamase genes were identified in 23 isolates (8.9%, 23\/260). Four transconjugants from different donors carrying <jats:italic>bla<\/jats:italic><jats:sub>CARB-3<\/jats:sub> exhibited a phenotype of reduced susceptibility to piperacillin\u2013tazobactam, ceftazidime, and cefepime, and 2 transconjugants harboring <jats:italic>bla<\/jats:italic><jats:sub>IMP-45<\/jats:sub> exhibited a phenotype of reduced susceptibility to carbapenems. <jats:italic>bla<\/jats:italic><jats:sub>CARB<\/jats:sub> positive isolates (<jats:italic>n<\/jats:italic>\u2009=\u200912) presented six PFGE patterns, designated groups A to F. Two <jats:italic>bla<\/jats:italic> genes (<jats:italic>bla<\/jats:italic><jats:sub>IMP-45<\/jats:sub> and <jats:italic>bla<\/jats:italic><jats:sub>OXA-1<\/jats:sub>) in PA1609 related to a class 1 integron (<jats:italic>intI1<\/jats:italic>-<jats:italic>bla<\/jats:italic><jats:sub>IMP-45-<\/jats:sub><jats:italic>bla<\/jats:italic><jats:sub>OXA-1<\/jats:sub>-<jats:italic>aac(6\u2032)-Ib7<\/jats:italic>-<jats:italic>catB3<\/jats:italic>-<jats:italic>qacE\u22061<\/jats:italic>-<jats:italic>sul1<\/jats:italic>) were encoded on a plasmid (pPA1609-475), while the <jats:italic>bla<\/jats:italic><jats:sub>CARB-3<\/jats:sub> gene of PA1616 also related to a class 1 integron was located on the chromosome. The results suggest that \u03b2-lactam antibiotic resistance and clonal dissemination exist in this hospital population. It indicates the necessity for molecular surveillance in tracking \u03b2-lactamase-producing strains and emphasizes the need for epidemiological monitoring.<\/jats:p>","DOI":"10.3389\/fmicb.2022.855961","type":"journal-article","created":{"date-parts":[[2022,4,28]],"date-time":"2022-04-28T09:20:06Z","timestamp":1651137606000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular Mechanism of the \u03b2-Lactamase Mediated \u03b2-Lactam Antibiotic Resistance of Pseudomonas aeruginosa Isolated From a Chinese Teaching Hospital"],"prefix":"10.3389","volume":"13","author":[{"given":"Hailong","family":"Lin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlin","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anqi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoling","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueya","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junwan","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kewei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teng","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changchong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyu","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,4,28]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"31","DOI":"10.18683\/germs.2018.1130","article-title":"Antimicrobial resistance and putative virulence genes of Pseudomonas aeruginosa isolates from patients with respiratory tract infection","volume":"8","author":"Al Dawodeyah","year":"2018","journal-title":"GERMS"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1128\/JCM.33.3.551-555.1995","article-title":"Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus","volume":"33","author":"Bannerman","year":"1995","journal-title":"J. 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