{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T19:18:11Z","timestamp":1789067891974,"version":"build-2803163510"},"reference-count":62,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T00:00:00Z","timestamp":1648425600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["VOLCANO 669371"],"award-info":[{"award-number":["VOLCANO 669371"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["Eco_MoM 339880"],"award-info":[{"award-number":["Eco_MoM 339880"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Methanotrophs aerobically oxidize methane to carbon dioxide to make a living and are known to degrade various other short chain carbon compounds as well. Volatile organic sulfur compounds such as methanethiol (CH<jats:sub>3<\/jats:sub>SH) are important intermediates in the sulfur cycle. Although volatile organic sulfur compounds co-occur with methane in various environments, little is known about how these compounds affect methanotrophy. The enzyme methanethiol oxidase catalyzing the oxidation of methanethiol has been known for decades, but only recently the <jats:italic>mtoX<\/jats:italic> gene encoding this enzyme was identified in a methylotrophic bacterium. The presence of a homologous gene in verrucomicrobial methanotrophs prompted us to examine how methanotrophs cope with methanethiol. Here, we show that the verrucomicrobial methanotroph <jats:italic>Methylacidiphilum fumariolicum<\/jats:italic> SolV consumes methanethiol and produces H<jats:sub>2<\/jats:sub>S, which is concurrently oxidized. Consumption of methanethiol is required since methanethiol inhibits methane oxidation. Cells incubated with \u223c15 \u03bcM methanethiol from the start clearly showed inhibition of growth. After depletion of methanethiol, growth resumed within 1 day. Genes encoding a putative methanethiol oxidase were found in a variety of methanotrophs. Therefore, we hypothesize that methanethiol degradation is a widespread detoxification mechanism in methanotrophs in a range of environments.<\/jats:p>","DOI":"10.3389\/fmicb.2022.857442","type":"journal-article","created":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T07:47:47Z","timestamp":1648626467000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":26,"title":["Methanethiol Consumption and Hydrogen Sulfide Production by the Thermoacidophilic Methanotroph Methylacidiphilum fumariolicum SolV"],"prefix":"10.3389","volume":"13","author":[{"given":"Rob A.","family":"Schmitz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sepehr S.","family":"Mohammadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo","family":"van Erven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Berben","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike S. M.","family":"Jetten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arjan","family":"Pol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huub J. M.","family":"Op den Camp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1038\/s41587-019-0036-z","article-title":"SignalP 5.0 improves signal peptide predictions using deep neural networks.","volume":"37","author":"Almagro Armenteros","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"3636","DOI":"10.1038\/s41396-021-01037-2","article-title":"Verrucomicrobial methanotrophs grow on diverse C3 compounds and use a homolog of particulate methane monooxygenase to oxidize acetone.","volume":"15","author":"Awala","year":"2021","journal-title":"ISME J."},{"key":"B3","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/0166-445X(92)90015-F","article-title":"Sulfide as an environmental factor and toxicant: tolerance and adaptations in aquatic organisms.","volume":"24","author":"Bagarinao","year":"1992","journal-title":"Aquat. Toxicol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1007\/s00792-007-0108-8","article-title":"Production of methanethiol and volatile sulfur compounds by the archaeon \u201cFerroplasma acidarmanus\u201d.","volume":"11","author":"Baumler","year":"2007","journal-title":"Extremophiles"},{"key":"B5","doi-asserted-by":"publisher","first-page":"314","DOI":"10.1177\/0734242X0101900","article-title":"Inhibition of methane oxidation by volatile sulfur compounds (CH3SH and CS2) in landfill cover soils.","volume":"19","author":"B\u00f6rjesson","year":"2001","journal-title":"Waste Manage. Res."},{"key":"B6","doi-asserted-by":"publisher","first-page":"1325","DOI":"10.1111\/gcb.12131","article-title":"Methane emissions from wetlands: biogeochemical, microbial, and modelling perspectives from local to global scales.","volume":"19","author":"Bridgham","year":"2013","journal-title":"Glob. Chang. Biol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1007\/978-3-319-98836-8_6","article-title":"Methanogens in the digestive tract of termites","author":"Brune","year":"2010","journal-title":"(Endo)symbiotic Methanogenic Archaea"},{"key":"B8","doi-asserted-by":"publisher","first-page":"2379","DOI":"10.1038\/ismej.2017.105","article-title":"Methanethiol-dependent dimethylsulfide production in soil environments.","volume":"11","author":"Carri\u00f3n","year":"2017","journal-title":"ISME J."},{"key":"B9","doi-asserted-by":"publisher","first-page":"2603","DOI":"10.1111\/j.1462-2920.2011.02464.x","article-title":"Modularity of methylotrophy, revisited.","volume":"13","author":"Chistoserdova","year":"2011","journal-title":"Environ. Microbiol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1016\/j.tim.2018.01.011","article-title":"Current trends in methylotrophy.","volume":"26","author":"Chistoserdova","year":"2018","journal-title":"Trends Microbiol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1002\/2017RG000559","article-title":"Methane feedbacks to the global climate system in a warmer world.","volume":"56","author":"Dean","year":"2018","journal-title":"Rev. Geophys."},{"key":"B12","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1038\/nature06411","article-title":"Methane oxidation by an extremely acidophilic bacterium of the phylum Verrucomicrobia.","volume":"450","author":"Dunfield","year":"2007","journal-title":"Nature"},{"key":"B13","doi-asserted-by":"publisher","first-page":"1129","DOI":"10.3389\/fmicb.2019.01129","article-title":"Global biogeographic distribution patterns of thermoacidophilic Verrucomicrobia methanotrophs suggest allopatric evolution.","volume":"10","author":"Erikstad","year":"2019","journal-title":"Front. Microbiol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"1430","DOI":"10.1016\/j.atmosenv.2008.03.014","article-title":"Natural emissions of methane from geological seepage in Europe.","volume":"43","author":"Etiope","year":"2009","journal-title":"Atmos. Environ."},{"key":"B15","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1038\/nature08883","article-title":"Nitrite-driven anaerobic methane oxidation by oxygenic bacteria.","volume":"464","author":"Ettwig","year":"2010","journal-title":"Nature"},{"key":"B16","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1038\/ismej.2017.148","article-title":"Bacterial SBP56 identified as a Cu-dependent methanethiol oxidase widely distributed in the biosphere.","volume":"12","author":"Eyice","year":"2018","journal-title":"ISME J."},{"key":"B17","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1007\/BF00249099","article-title":"Fermentation of methanethiol and dimethylsulfide by a newly isolated methanogenic bacterium.","volume":"157","author":"Finster","year":"1992","journal-title":"Arch. Microbiol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1099\/00221287-138-1-217","article-title":"Purification and properties of methyl mercaptan oxidase from Thiobacillus thioparus TK-m.","volume":"138","author":"Gould","year":"1992","journal-title":"J. Gen. Microbiol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1128\/mr.60.2.439-471.1996","article-title":"Methanotrophic bacteria.","volume":"60","author":"Hanson","year":"1996","journal-title":"Microbiol. Rev."},{"key":"B20","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1038\/nature12375","article-title":"Anaerobic oxidation of methane coupled to nitrate reduction in a novel archaeal lineage.","volume":"500","author":"Haroon","year":"2013","journal-title":"Nature"},{"key":"B21","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1007\/s10584-013-0986-","article-title":"Tracing anthropogenic carbon dioxide and methane emissions to fossil fuel and cement producers, 1854\u20132014.","volume":"122","author":"Heede","year":"2014","journal-title":"Clim. Change"},{"key":"B22","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1007\/BF01809852","article-title":"Organic sulfur compounds resulting from the interaction of iron sulfide, hydrogen sulfide and carbon dioxide in an anaerobic aqueous environment.","volume":"26","author":"Heinen","year":"1995","journal-title":"Orig. Life Evol. Biosph."},{"key":"B23","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1073\/pnas.0704162105","article-title":"Methane oxidation at 55\u00b0C and pH 2 by a thermoacidophilic bacterium belonging to the Verrucomicrobia phylum.","volume":"105","author":"Islam","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B24","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/0304-4203(96)00006-0","article-title":"Production of methanethiol from dimethylsulfoniopropionate in marine surface waters.","volume":"54","author":"Kiene","year":"1996","journal-title":"Mar. Chem."},{"key":"B25","doi-asserted-by":"publisher","first-page":"1103","DOI":"10.1016\/j.atmosenv.2004.09.083","article-title":"Characterization of malodorous sulfur compounds in landfill gas.","volume":"39","author":"Kim","year":"2005","journal-title":"Atmos. Environ."},{"key":"B26","doi-asserted-by":"publisher","first-page":"123002","DOI":"10.1016\/j.jhazmat.2020.123002","article-title":"Effect of methanethiol on process performance, selectivity and diversity of sulfur-oxidizing bacteria in a dual bioreactor gas biodesulfurization system.","volume":"398","author":"Kiragosyan","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"B27","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1146\/annurev.micro.61.080706.093130","article-title":"Anaerobic oxidation of methane: progress with an unknown process.","volume":"63","author":"Knittel","year":"2009","journal-title":"Annu. Rev. Microbiol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"949","DOI":"10.1002\/cbic.202000661","article-title":"Hydrogen sulfide oxidation by sulfide quinone oxidoreductase.","volume":"22","author":"Landry","year":"2021","journal-title":"ChemBioChem"},{"key":"B29","doi-asserted-by":"publisher","first-page":"670","DOI":"10.1016\/j.jbiosc.2015.04.006","article-title":"Inhibitory effects of sulfur compounds on methane oxidation by a methane-oxidizing consortium.","volume":"120","author":"Lee","year":"2015","journal-title":"J. Biosci. Bioeng."},{"key":"B30","doi-asserted-by":"publisher","first-page":"753","DOI":"10.4014\/jmb.1102.01055","article-title":"Characterization of methane oxidation by a methanotroph isolated from a landfill cover soil, South Korea.","volume":"21","author":"Lee","year":"2011","journal-title":"J. Microbiol. Biotechnol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1016\/j.jhazmat.2012.07.047","article-title":"Isolation and characterization of a facultative methanotroph degrading malodor-causing volatile sulfur compounds.","author":"Lee","year":"2012","journal-title":"J. Hazard. Mater."},{"key":"B32","doi-asserted-by":"publisher","first-page":"4017","DOI":"10.1128\/AEM.67.9.4017-4023.2001","article-title":"Obligate sulfide-dependent degradation of methoxylated aromatic compounds and formation of methanethiol and dimethyl sulfide by a freshwater sediment isolate, Parasporobacterium paucivorans gen. nov., sp. nov.","volume":"67","author":"Lomans","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"3641","DOI":"10.1128\/AEM.65.8.3641-3650.1999","article-title":"Isolation and characterization of Methanomethylovorans hollandica gen. nov., sp. nov., isolated from freshwater sediment, a methylotrophic methanogen able to grow on dimethyl sulfide and methanethiol.","volume":"65","author":"Lomans","year":"1999","journal-title":"Appl. Environ. Microbiol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"4741","DOI":"10.1128\/aem.63.12.4741-4747.1997","article-title":"Formation of dimethyl sulfide and methanethiol in anoxic freshwater sediments.","volume":"63","author":"Lomans","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1007\/s00018-002-84","article-title":"Microbial cycling of volatile organic sulfur compounds.","volume":"59","author":"Lomans","year":"2002","journal-title":"Cell. Mol. Life Sci."},{"key":"B36","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1111\/j.1574-6941.2009.00765.x","article-title":"Anaerobic oxidation of dimethylsulfide and methanethiol in mangrove sediments is dominated by sulfate-reducing bacteria.","volume":"70","author":"Lyimo","year":"2009","journal-title":"FEMS Microbiol. Ecol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1099\/00207713-50-1-171","article-title":"Methanosarcina semesiae sp. nov., a dimethylsulfide-utilizing methanogen from mangrove sediment.","volume":"50","author":"Lyimo","year":"2000","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1007\/s13181-020-00767-7","article-title":"Efficacy of intravenous hydroxocobalamin for treatment of sodium methanethiolate exposure in a swine model (Sus scrofa) of severe methanethiol toxicity.","volume":"16","author":"Maddry","year":"2020","journal-title":"J. Med. Toxicol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"W636","DOI":"10.1093\/nar\/gkz268","article-title":"The EMBL-EBI search and sequence analysis tools APIs in 2019.","volume":"47","author":"Madeira","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"B40","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1038\/s42003-020-0838-z","article-title":"Growth and rapid succession of methanotrophs effectively limit methane release during lake overturn.","volume":"3","author":"Mayr","year":"2020","journal-title":"Commun. Biol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"945","DOI":"10.1038\/ismej.2016.171","article-title":"Methylacidiphilum fumariolicum SolV, a thermoacidophilic \u2018Knallgas\u2019 methanotroph with both an oxygen-sensitive and -insensitive hydrogenase.","volume":"11","author":"Mohammadi","year":"2017","journal-title":"ISME J."},{"key":"B42","doi-asserted-by":"publisher","first-page":"2352","DOI":"10.3389\/fmicb.2019.02352","article-title":"The acidophilic methanotroph Methylacidimicrobium tartarophylax 4AC grows as autotroph on H2 under microoxic conditions.","volume":"10","author":"Mohammadi","year":"2019","journal-title":"Front. Microbiol."},{"key":"B43","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1111\/j.1758-2229.2009.00089.x","article-title":"The microbial methane cycle.","volume":"1","author":"Murrell","year":"2009","journal-title":"Environ. Microbiol. Rep."},{"key":"B44","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1111\/j.1758-2229.2009.00022.x","article-title":"Environmental, genomic and taxonomic perspectives on methanotrophic Verrucomicrobia.","volume":"1","author":"Op den Camp","year":"2009","journal-title":"Environ. Microbiol. Rep."},{"key":"B45","doi-asserted-by":"publisher","first-page":"604485","DOI":"10.3389\/fmicb.2020.604485","article-title":"More than a methanotroph: a broader substrate spectrum for Methylacidiphilum fumariolicum SolV.","volume":"11","author":"Picone","year":"2020","journal-title":"Front. Microbiol."},{"key":"B46","doi-asserted-by":"publisher","first-page":"874","DOI":"10.1038\/nature06222","article-title":"Methanotrophy below pH 1 by a new Verrucomicrobia species.","volume":"450","author":"Pol","year":"","journal-title":"Nature"},{"key":"B47","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1007\/s00253-006-0663-4","article-title":"Isolation of a carbon disulfide utilizing Thiomonas sp. and its application in a biotrickling filter.","volume":"74","author":"Pol","year":"","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"B48","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1007\/BF00700635","article-title":"Isolation of a dimethyl sulfide utilizing Hyphomicrobium species and its application in biofiltration of polluted air.","volume":"5","author":"Pol","year":"1994","journal-title":"Biodegradation"},{"key":"B49","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1038\/s41588-017-0006-7","article-title":"Mutations in SELENBP1, encoding a novel human methanethiol oxidase, cause extraoral halitosis.","volume":"50","author":"Pol","year":"2018","journal-title":"Nat. Genet."},{"key":"B50","doi-asserted-by":"publisher","first-page":"5474","DOI":"10.1073\/pnas.1400643111","article-title":"The origin of methanethiol in midocean ridge hydrothermal fluids.","volume":"111","author":"Reeves","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B51","doi-asserted-by":"publisher","first-page":"1693","DOI":"10.1080\/09593330.2015.1128001","article-title":"Influence of methanethiol on biological sulphide oxidation in gas treatment system.","volume":"37","author":"Roman","year":"2016","journal-title":"Environ. Technol."},{"key":"B52","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1007\/s00775-016-1419-y","article-title":"A tale of two methane monooxygenases.","volume":"22","author":"Ross","year":"2017","journal-title":"J. Biol. Inorg. Chem."},{"key":"B53","doi-asserted-by":"publisher","first-page":"173","DOI":"10.21775\/cimb.033.173","article-title":"Microbial cycling of methanethiol.","volume":"33","author":"Sch\u00e4fer","year":"2019","journal-title":"Curr. Issues Mol. Biol."},{"key":"B54","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1093\/jxb\/erp355","article-title":"Microbial degradation of dimethylsulfide and related C1-sulphur compounds: organisms and pathways controlling fluxes of sulphur in the biosphere.","volume":"61","author":"Sch\u00e4fer","year":"2010","journal-title":"J. Exp. Bot."},{"key":"B55","doi-asserted-by":"publisher","first-page":"fuab007","DOI":"10.1093\/femsre\/fuab007","article-title":"Verrucomicrobial methanotrophs: ecophysiology of metabolically versatile acidophiles.","volume":"45","author":"Schmitz","year":"2021","journal-title":"FEMS Microbiol. Rev."},{"key":"B56","doi-asserted-by":"publisher","first-page":"1223","DOI":"10.1038\/s41396-020-0609-3","article-title":"The thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV oxidizes subatmospheric H2 with a high-affinity, membrane-associated [NiFe] hydrogenase.","volume":"14","author":"Schmitz","year":"2020","journal-title":"ISME J."},{"key":"B57","doi-asserted-by":"publisher","first-page":"6023","DOI":"10.1029\/91JD03139","article-title":"Global inventory of sulfur emissions with 1\u00b0x1\u00b0 resolution.","volume":"97","author":"Spiro","year":"1992","journal-title":"J. Geophys. Res. Atmos."},{"key":"B58","doi-asserted-by":"publisher","first-page":"1312","DOI":"10.1093\/bioinformatics\/btu033","article-title":"RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies.","volume":"30","author":"Stamatakis","year":"2014","journal-title":"Bioinformatics"},{"key":"B59","doi-asserted-by":"publisher","first-page":"2989","DOI":"10.1099\/00221287-133-11-2989","article-title":"Methyl mercaptan oxidase, a key enzyme in the metabolism of methylated sulfur-compounds by Hyphomicrobium EG.","volume":"133","author":"Suylen","year":"1987","journal-title":"J. Gen. Microbiol."},{"key":"B60","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1007\/s00253-009-1951-6","article-title":"Inhibition of microbiological sulfide oxidation by methanethiol and dimethyl polysulfides at natronalkaline conditions.","volume":"83","author":"van den Bosch","year":"2009","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"B61","doi-asserted-by":"publisher","first-page":"6782","DOI":"10.1128\/AEM.01838-14","article-title":"Expanding the verrucomicrobial methanotrophic world: description of three novel species of Methylacidimicrobium gen. nov.","volume":"80","author":"van Teeseling","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"key":"B62","doi-asserted-by":"publisher","first-page":"1672","DOI":"10.3389\/fmicb.2018.01672","article-title":"Comparative genomics of Candidatus Methylomirabilis species and description of Ca. Methylomirabilis lanthanidiphila.","volume":"9","author":"Versantvoort","year":"2018","journal-title":"Front. Microbiol."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.857442\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T07:47:50Z","timestamp":1648626470000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.857442\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,28]]},"references-count":62,"alternative-id":["10.3389\/fmicb.2022.857442"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2022.857442","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,28]]},"article-number":"857442"}}