{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T22:54:54Z","timestamp":1782860094745,"version":"3.54.5"},"reference-count":77,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T00:00:00Z","timestamp":1649203200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006013","name":"United Arab Emirates University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006013","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>The study aimed to determine the prevalence of different species of <jats:italic>Vibrio<\/jats:italic> spp. in fish and shellfish sold in subtropical-arid countries (United Arab Emirates). It also examined the antimicrobial resistance of the isolated species and their growth behavior upon <jats:italic>in vitro<\/jats:italic> environmental changes concerning temperature, pH, and salinity. The prevalence of <jats:italic>Vibrio<\/jats:italic> spp. in fish and shellfish samples, was 64.5 and 92%, respectively. However, <jats:italic>Vibrio parahemolyticus<\/jats:italic> were detected in a mere 7.5 and 13.0% of the samples, respectively. On the other hand, <jats:italic>Vibrio mimicus<\/jats:italic> was detected in 1.5 and 8.5% of the samples, respectively. None of the six antibiotics studied except for Sulfamethoxazole-trimethoprim were effective against fish <jats:italic>Vibrio<\/jats:italic> spp. isolates. On a similar note, three antibiotics, namely Penicillin, Daptomycin, and Vancomycin, were ineffective against the shellfish isolates. The growth of the microorganisms did not show any significant trend with changes in pH and salinity. The optimum temperature for <jats:italic>Vibrio<\/jats:italic> spp. growth was observed to be 37\u00b0C.<\/jats:p>","DOI":"10.3389\/fmicb.2022.861547","type":"journal-article","created":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T05:54:23Z","timestamp":1649224463000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Prevalence, Antibiotic-Resistance, and Growth Profile of Vibrio spp. Isolated From Fish and Shellfish in Subtropical-Arid Area"],"prefix":"10.3389","volume":"13","author":[{"given":"Tarfa","family":"Abdalla","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hind","family":"Al-Rumaithi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tareq M.","family":"Osaili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fayeza","family":"Hasan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reyad S.","family":"Obaid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aisha","family":"Abushelaibi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mutamed M.","family":"Ayyash","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,4,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1016\/j.foodcont.2013.03.024","article-title":"Occurrence and molecular identification of Vibrio parahaemolyticus in retail shellfish in Mansoura, Egypt","volume":"33","author":"Abd-Elghany","year":"2013","journal-title":"Food Control"},{"key":"ref2","first-page":"985","article-title":"Incidence of potentially pathogenic Vibrio spp., in fresh seafood from Itu Creek in Uyo, Akwa Ibom state, Nigeria","volume":"15","author":"Adebayo-Tayo","year":"2011","journal-title":"World Appl. Sci. J."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1186\/1471-2334-14-421","article-title":"Water sources as reservoirs of vibrio cholerae O1 and non-O1 strains in Bepanda, Douala (Cameroon): relationship between isolation and physico-chemical factors","volume":"14","author":"Akoachere","year":"2014","journal-title":"BMC Infect. Dis."},{"key":"ref4","first-page":"63","article-title":"Vibrio detection in market seafood samples of Kuwait by biochemical (API 20E) strips and its evaluation against 16s rDNA-based molecular methods","volume":"7","author":"Al-Mouqati","year":"2012","journal-title":"Res. J. Biotechnol."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"1355","DOI":"10.22207\/JPAM.12.3.38","article-title":"Characterization of Vibrio vulnificus isolated from the coastal areas in the Eastern Province of Saudi Arabia","volume":"12","author":"Alsalem","year":"2018","journal-title":"J. Pure Appl. Microbiol."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1186\/s12866-019-1624-2","article-title":"Prevalence, antimicrobial susceptibility and plasmid profiling of Vibrio spp., isolated from cultured groupers in Peninsular Malaysia","volume":"19","author":"Amalina","year":"2019","journal-title":"BMC Microbiol."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"46","DOI":"10.2174\/1874070701812010046","article-title":"Detection and molecular characterization of Vibrio parahaemolyticus in shrimp samples","volume":"12","author":"Asgarpoor","year":"2018","journal-title":"Open Biotechnol. J."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1006\/fmic.2001.0441","article-title":"Determination of several potential virulence factors in vibrio spp., Isolated from Sea Water","volume":"18","author":"Baffone","year":"2001","journal-title":"Food Microbiol."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1007\/s00248-008-9413-8","article-title":"Multi-site analysis reveals widespread antibiotic resistance in the marine pathogen Vibrio vulnificus","volume":"57","author":"Baker-Austin","year":"2009","journal-title":"Microb. Ecol."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41572-018-0005-8","article-title":"Vibrio spp. Infections","volume":"4","author":"Baker-Austin","year":"2018","journal-title":"Nat. Rev. Dis. Prim."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1111\/jfd.13270","article-title":"Impact of a reduced water salinity on the composition of Vibrio spp., in recirculating aquaculture systems for Pacific white shrimp (Litopenaeus vannamei) and its possible risks for shrimp health and food safety","volume":"44","author":"Bauer","year":"2021","journal-title":"J. Fish Dis."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"844","DOI":"10.1128\/am.25.5.844-846.1973","article-title":"Interacting effects of pH, temperature, and salt concentration on growth and survival of Vibrio parahaemolyticus","volume":"25","author":"Beuchat","year":"1973","journal-title":"Appl. Microbiol."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1111\/j.1365-2672.2008.03974.x","article-title":"Comparison of Vibrio spp., populations found in seawater, in exhibition aquaria, in fish intestine and in fish feed","volume":"106","author":"Blanch","year":"2009","journal-title":"J. Appl. Microbiol."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1080\/10408398.2017.1384715","article-title":"Vibrio species involved in seafood-borne outbreaks (Vibrio cholerae, V. parahaemolyticus and V. vulnificus): review of microbiological versus recent molecular detection methods in seafood products","volume":"59","author":"Bonnin-Jusserand","year":"2019","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1111\/j.1745-4565.2003.tb00361.x","article-title":"Novel nonthermal methods to reduce Vibrio vulnificus in raw oysters","volume":"23","author":"Borazjani","year":"2003","journal-title":"J. Food Saf."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"992","DOI":"10.1016\/j.micinf.2011.06.013","article-title":"Vibrio parahaemolyticus cell biology and pathogenicity determinants","volume":"13","author":"Broberg","year":"2011","journal-title":"Microbes Infect."},{"key":"ref17","year":"2018"},{"key":"ref18","year":""},{"key":"ref19","year":""},{"key":"ref20","year":""},{"key":"ref21","year":""},{"key":"ref22","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1111\/j.1348-0421.2004.tb03513.x","article-title":"Emergence and serovar transition of Vibrio parahaemolyticus pandemic strains isolated during a diarrhea outbreak in Vietnam between 1997 and 1999","volume":"48","author":"Chowdhury","year":"2004","journal-title":"Microbiol. Immunol."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"648","DOI":"10.1016\/j.foodcont.2005.04.014","article-title":"Occurrence of Vibrio spp., and Aeromonas spp., in shellfish harvested off Dardanelles cost of Turkey","volume":"17","author":"Colakoglu","year":"2006","journal-title":"Food Control"},{"key":"ref24","year":"2021"},{"key":"ref25","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/S0721-9571(81)80019-1","article-title":"Growth of vibrio alginolyticus: interacting effects on pH, temperature, salt concentration, and incubation time","volume":"2","author":"Farid","year":"1981","journal-title":"Zentralblatt f\u00fcr Bakteriologie, Mikrobiologie und Hygiene"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1111\/j.1574-6968.2004.tb09528.x","article-title":"Significance of Na+ in the fish pathogen, Vibrio anguillarum, under energy depleted condition","volume":"234","author":"Fujiwara-Nagata","year":"2004","journal-title":"FEMS Microbiol. Lett."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1111\/j.1574-6941.2012.01427.x","article-title":"Virulence genes and pathogenicity islands in environmental Vibrio strains nonpathogenic to humans","volume":"82","author":"Gennari","year":"2012","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/j.ijfoodmicro.2004.12.011","article-title":"The occurrence of Vibrio species in tropical shrimp culture environments; implications for food safety","volume":"102","author":"Gopal","year":"2005","journal-title":"Int. J. Food Microbiol."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"21134","DOI":"10.1073\/pnas.1013825107","article-title":"Comparative genomics of clinical and environmental Vibrio mimicus","volume":"107","author":"Hasan","year":"2010","journal-title":"Proc. Natl. Acad. Sci."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"108876","DOI":"10.1016\/j.foodcont.2022.108876","article-title":"Preservative effect of slightly acid electrolysed water ice generated by the developed sanitising unit on shrimp (Penaeus vannamei)","volume":"136","author":"He","year":"2022","journal-title":"Food Control"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1002\/1615-9861(200205)2:5<571::AID-PROT571>3.0.CO;2-G","article-title":"Effect of mild acid pH on the functioning of bacterial membranes in vibrio cholerae","volume":"2","author":"Hommais","year":"2002","journal-title":"Proteomics"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"1582","DOI":"10.1080\/21505594.2020.1845039","article-title":"Pathogenicity and virulence regulation of Vibrio cholerae at the interface of host-gut microbiome interactions","volume":"11","author":"Hsiao","year":"2020","journal-title":"Virulence"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"234","DOI":"10.21608\/bvmj.2018.29434","article-title":"Effect of marination on Vibrio parahaemolyticus in tilapia fillets","volume":"34","author":"Ibrahim","year":"2018","journal-title":"Benha Vet. Med. J."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1089\/mdr.2015.0169","article-title":"Detection and antimicrobial resistance of Vibrio isolates in aquaculture environments: implications for public health","volume":"22","author":"Igbinosa","year":"2016","journal-title":"Microb. Drug Resist."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1089\/fpd.2011.1018","article-title":"Isolation, molecular characterization, and antibiotic susceptibility of Vibrio parahaemolyticus in Korean seafood","volume":"9","author":"Jun","year":"2012","journal-title":"Foodborne Pathog. Dis."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1016\/j.bioelechem.2008.04.012","article-title":"The temperature effect during pulse application on cell membrane fluidity and permeabilization","volume":"74","author":"Kandu\u0161er","year":"2008","journal-title":"Bioelectrochemistry"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/j.marpolbul.2017.02.070","article-title":"Characterization of Vibrio parahaemolyticus isolated from oysters in Korea: resistance to various antibiotics and prevalence of virulence genes","volume":"118","author":"Kang","year":"2017","journal-title":"Mar. Pollut. Bull."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"147","DOI":"10.3389\/fmars.2017.00147","article-title":"Correlation of total bacterial and vibrio spp., populations between fish and water in the aquaculture system","volume":"4","author":"Kim","year":"2017","journal-title":"Front. Mar. Sci."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"4662","DOI":"10.3390\/ijerph9124662","article-title":"Effect of temperature on growth of Vibrio paraphemolyticus and Vibrio vulnificus in flounder, salmon sashimi and oyster meat","volume":"9","author":"Kim","year":"2012","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"1173","DOI":"10.1128\/JCM.37.4.1173-1177.1999","article-title":"Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene","volume":"37","author":"Kim","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1099\/jmm.0.023051-0","article-title":"Antibiotic resistance mechanisms of Vibrio cholerae","volume":"60","author":"Kitaoka","year":"2011","journal-title":"J. Med. Microbiol."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"232","DOI":"10.3389\/fpubh.2015.00232","article-title":"Occurrence and diversity of clinically important Vibrio species in the aquatic environment of Georgia","volume":"3","author":"Kokashvili","year":"2015","journal-title":"Front. Public Health"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1186\/1471-2334-6-130","article-title":"How long do nosocomial pathogens persist on inanimate surfaces? A systematic review","volume":"6","author":"Kramer","year":"2006","journal-title":"BMC Infect. Dis."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"68","DOI":"10.3390\/antibiotics8020068","article-title":"Multiple antibiotic resistance (MAR), plasmid profiles, and DNA polymorphisms among Vibrio vulnificus isolates","volume":"8","author":"Kurdi Al-Dulaimi","year":"2019","journal-title":"Antibiotics"},{"key":"ref45","doi-asserted-by":"publisher","first-page":"33","DOI":"10.3389\/fmicb.2015.00033","article-title":"Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from retail shrimps in Malaysia","volume":"6","author":"Letchumanan","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"1029","DOI":"10.4315\/0362-028X.JFP-14-437","article-title":"Prevalence and antimicrobial resistance of Vibrio parahaemolyticus isolated from raw shellfish in Poland","volume":"78","author":"Lopatek","year":"2015","journal-title":"J. Food Prot."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"736","DOI":"10.3389\/fmicb.2015.00736","article-title":"Prevalences of pathogenic and non-pathogenic Vibrio parahaemolyticus in mollusks from the Spanish Mediterranean coast","volume":"6","author":"Lopez-Joven","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.fm.2017.10.016","article-title":"Temperature affects the growth and survival of tdh positive Vibrio parahaemolyticus in tissues of postharvest Manila clam (Ruditapes philippinarum)","volume":"75","author":"Lopez-Joven","year":"2018","journal-title":"Food Microbiol."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"107697","DOI":"10.1016\/j.foodcont.2020.107697","article-title":"Changes of metabolite profiles of fish models inoculated with Shewanella baltica during spoilage","volume":"123","author":"Lou","year":"2021","journal-title":"Food Control"},{"key":"ref50","doi-asserted-by":"publisher","first-page":"e0169521","DOI":"10.1371\/journal.pone.0169521","article-title":"Impact of salt and nutrient content on biofilm formation by Vibrio fischeri","volume":"12","author":"Marsden","year":"2017","journal-title":"PLoS One"},{"key":"ref51","first-page":"51","article-title":"The multiple identities of Vibrio parahaemolyticus","volume":"1","author":"McCarter","year":"1999","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/S0168-1605(97)00100-1","article-title":"Development and evaluation of a predictive model for the effect of temperature and water activity on the growth rate of Vibrio parahaemolyticus","volume":"38","author":"Miles","year":"1997","journal-title":"Int. J. Food Microbiol."},{"key":"ref53","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s13213-015-1102-5","article-title":"Major foodborne pathogens in fish and fish products: a review","volume":"66","author":"Novoslavskij","year":"2016","journal-title":"Ann. Microbiol."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1186\/s40064-016-2115-7","article-title":"Incidence and prevalence of Vibrio parahaemolyticus in seafood: a systematic review and meta-analysis","volume":"5","author":"Odeyemi","year":"2016","journal-title":"Springerplus"},{"key":"ref55","first-page":"944","article-title":"Occurrence of Vibrio spp., Aeromonas hydrophila, Escherichia coli and Campylobacter spp., in crayfish (Astacus leptodactylus) from Iran","volume":"13","author":"Raissy","year":"2014","journal-title":"Iran. J. Fish. Sci."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"e4035","DOI":"10.3791\/4035","article-title":"A semi-quantitative approach to assess biofilm formation using wrinkled colony development","volume":"64","author":"Ray","year":"2012","journal-title":"J. Vis. Exp."},{"key":"ref57","first-page":"77","article-title":"Environmental impacts of shellfish aquaculture: filter feeding to control eutrophication","volume-title":"Marine Aquaculture and the Environment: A Meeting for Stakeholders in the Northeast","author":"Rice","year":"2001"},{"key":"ref58","year":"2020"},{"key":"ref59","doi-asserted-by":"publisher","first-page":"2292","DOI":"10.3389\/fimmu.2019.02292","article-title":"A comparison of the innate and adaptive immune systems in cartilaginous fish, ray-finned fish, and lobe-finned fish","volume":"10","author":"Smith","year":"2019","journal-title":"Front. Immunol."},{"key":"ref60","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1016\/j.foodcont.2016.08.041","article-title":"Effect of temperature on pathogenic and non-pathogenic Vibrio parahaemolyticus biofilm formation","volume":"73","author":"Song","year":"2017","journal-title":"Food Control"},{"key":"ref61","doi-asserted-by":"publisher","first-page":"89","DOI":"10.20431\/2349-0365.0406003","article-title":"Prevalence of toxic genes of Vibrio parahaemolyticus in shrimps (Penaeus monodon) and culture environment","volume":"16","author":"Sujeewa","year":"2009","journal-title":"Int. Food Res. J."},{"key":"ref62","first-page":"105","article-title":"Salt tolerance of bacteria isolated from tropical marine fish and prawn","volume":"20","author":"Surendran","year":"1983","journal-title":"Fish. Technol."},{"key":"ref63","doi-asserted-by":"publisher","first-page":"1087","DOI":"10.3389\/fmicb.2017.01087","article-title":"Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from short mackerels (Rastrelliger brachysoma) in Malaysia","volume":"8","author":"Tan","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref64","year":"2019"},{"key":"ref65","year":"2020"},{"key":"ref66","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2018\/2910481","article-title":"Temperature effect study on growth and survival of pathogenic Vibrio parahaemolyticus in Jinjiang oyster (Crassostrea rivularis) with rapid count method","volume":"2018","author":"Wang","year":"2018","journal-title":"J. Food Qual."},{"key":"ref67","doi-asserted-by":"publisher","first-page":"4720","DOI":"10.1128\/AEM.00474-10","article-title":"Modulation of responses of Vibrio parahaemolyticus O3: K6 to pH and temperature stresses by growth at different salt concentrations","volume":"76","author":"Whitaker","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref68","year":"2021"},{"key":"ref69","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/femsec\/fix071","article-title":"Different abundance and correlational patterns exist between total and presumed pathogenic Vibrio vulnificus and V. parahaemolyticus in shellfish and waters along the North Carolina coast","volume":"93","author":"Williams","year":"2017","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref70","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1016\/j.micpath.2016.07.014","article-title":"Isolation and identification of Vibrio cholerae and Vibrio parahaemolyticus from prawn (Penaeus monodon) seafood: preservation strategies","volume":"99","author":"Yaashikaa","year":"2016","journal-title":"Microb. Pathog."},{"key":"ref71","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/j.ijfoodmicro.2008.04.007","article-title":"Isolation and molecular characterization of Vibrio parahaemolyticus from fresh, low-temperature preserved, dried, and salted seafood products in two coastal areas of eastern China","volume":"125","author":"Yang","year":"2008","journal-title":"Int. J. Food Microbiol."},{"key":"ref72","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.ijfoodmicro.2009.11.006","article-title":"The low-salt stimulon in Vibrio parahaemolyticus","volume":"137","author":"Yang","year":"2010","journal-title":"Int. J. Food Microbiol."},{"key":"ref73","doi-asserted-by":"publisher","first-page":"829","DOI":"10.1007\/s10068-017-0105-3","article-title":"Effects of varying concentrations of sodium chloride and acidic conditions on the behavior of Vibrio parahaemolyticus and Vibrio vulnificus cold-starved in artificial sea water microcosms","volume":"26","author":"Yoon","year":"2017","journal-title":"Food Sci. Biotechnol."},{"key":"ref74","doi-asserted-by":"publisher","first-page":"2375","DOI":"10.21608\/avmj.2018.168732","article-title":"Molecular characterization of Vibrio parahaemolyticus isolated from shellfish and their harvesting water from Suez Canal area, Egypt","volume":"25","author":"Youssef","year":"2018","journal-title":"Int. Food Res. J."},{"key":"ref75","doi-asserted-by":"publisher","first-page":"380","DOI":"10.4315\/0362-028X-73.2.380","article-title":"Prevalence of Listeria, Aeromonas, and Vibrio species in fish used for human consumption in Turkey","volume":"73","author":"Y\u00fccel","year":"2010","journal-title":"J. Food Prot."},{"key":"ref76","doi-asserted-by":"publisher","first-page":"129581","DOI":"10.1016\/j.foodchem.2021.129581","article-title":"Effect of vacuum impregnated fish gelatin and grape seed extract on moisture state, microbiota composition, and quality of chilled seabass fillets","volume":"354","author":"Zhao","year":"2021","journal-title":"Food Chem."},{"key":"ref77","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1046\/j.1365-2761.2002.00383.x","article-title":"Ultrasonic immunization of sea bream, Pagrus major (Temminck & Schlegel), with a mixed vaccine against Vibrio alginolyticus and V. anguillarum","volume":"25","author":"Zhou","year":"2002","journal-title":"J. Fish Dis."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.861547\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T05:54:31Z","timestamp":1649224471000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.861547\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,6]]},"references-count":77,"alternative-id":["10.3389\/fmicb.2022.861547"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2022.861547","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,6]]},"article-number":"861547"}}