{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T22:34:38Z","timestamp":1776119678575,"version":"3.50.1"},"reference-count":70,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T00:00:00Z","timestamp":1657497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004530","name":"Universiti Putra Malaysia","doi-asserted-by":"publisher","award":["FRGS\/1\/2019\/STG03\/UPM\/01\/2"],"award-info":[{"award-number":["FRGS\/1\/2019\/STG03\/UPM\/01\/2"]}],"id":[{"id":"10.13039\/501100004530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Contagious ecthyma commonly known as Orf is a globally important, highly contagious zoonotic, transboundary disease that affects domestic and wild ruminants. The disease is of great economic significance causing an immense impact on animal health, welfare, productivity, and trade. Detailed analysis of the viral genome is crucial to further elucidate the molecular mechanism of Orf virus (ORFV) pathogenesis. In the present study, a confluent monolayer of lamb testicle cells was infected with the processed scab sample obtained from an infected goat. The presence of the virus was confirmed using polymerase chain reaction and electron microscopy, while its genome was sequenced using next-generation sequencing technology. The genome sequence of Malaysian ORFV strain UPM\/HSN-20 was found to contain 132,124\u2009bp with a G\u2009+\u2009C content of 63.7%. The homology analysis indicates that UPM\/HSN-20 has a high level of identity 97.3\u201399.0% with the other reference ORFV strain. Phylogenetic analysis revealed that ORFV strain UPM\/HSN-20 is genetically more closely related to ORFV strain XY and NP from China. The availability of the genome-wide analysis of ORFV UPM\/HSN-20 strain from Malaysia will serve as a good platform for further understanding of genetic diversity, ORFV infection, and strategic development for control measures.<\/jats:p>","DOI":"10.3389\/fmicb.2022.877149","type":"journal-article","created":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T06:00:52Z","timestamp":1657519252000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Genome-Wide Analysis and Molecular Characterization of Orf Virus Strain UPM\/HSN-20 Isolated From Goat in Malaysia"],"prefix":"10.3389","volume":"13","author":[{"given":"Hassana Kyari","family":"Mangga","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jamilu Abubakar","family":"Bala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krishnan Nair","family":"Balakrishnan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alhaji Modu","family":"Bukar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zaharaddeen","family":"Lawan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Auwal","family":"Gambo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faez Firdaus Abdullah","family":"Jesse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustapha M.","family":"Noordin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohd-Lila","family":"Mohd-Azmi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,7,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1007\/s13337-015-0278-4","article-title":"Isolation and phylogenetic analysis of caprine Orf virus in Malaysia","volume":"26","author":"Abdullah","year":"2015","journal-title":"VirusDisease"},{"key":"ref2","author":"Al-Ajeeli","year":"1995"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"2197","DOI":"10.3201\/eid2512.181513","article-title":"Human infection with Orf virus and description of its whole genome, France, 2017","volume":"25","author":"Andreani","year":"2019","journal-title":"Emerg. 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