{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T19:09:33Z","timestamp":1791572973343,"version":"4.3.4"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T00:00:00Z","timestamp":1655856000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Carbapenem-resistant <jats:italic>Klebsiella pneumoniae<\/jats:italic> (CRKP), a pathogen that causes severe nosocomial infections and yields a high mortality rate, poses a serious threat to global public health due to its high antimicrobial resistance. Bacteriophages encode polysaccharide-degrading enzymes referred to as depolymerases that cleave the capsular polysaccharide (CPS), one of the main virulence factors of <jats:italic>K. pneumoniae<\/jats:italic>. In this study, we identified and characterized a new capsule depolymerase K19-Dpo41 from <jats:italic>K. pneumoniae<\/jats:italic> bacteriophage SH-KP156570. Our characterization of K19-Dpo41 demonstrated that this depolymerase showed specific activities against K19-type <jats:italic>K. pneumoniae<\/jats:italic>. K19-Dpo41-mediated treatments promoted the sensitivity of a multidrug-resistant K19-type <jats:italic>K. pneumoniae<\/jats:italic> strain to the bactericidal effect of human serum and significantly increased the survival rate of <jats:italic>Galleria mellonella<\/jats:italic> infected with K19-type <jats:italic>K. pneumoniae<\/jats:italic>. Our results provided strong primary evidence that K19-Dpo41 was not only effective in capsular typing of K19-type <jats:italic>K. pneumoniae<\/jats:italic> but promising in terms of developing new alternative therapeutic strategies against K19-type CRKP infections in the future.<\/jats:p>","DOI":"10.3389\/fmicb.2022.878800","type":"journal-article","created":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T06:51:27Z","timestamp":1655880687000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":23,"title":["Characterization and Functional Studies of a Novel Depolymerase Against K19-Type Klebsiella pneumoniae"],"prefix":"10.3389","volume":"13","author":[{"given":"Yunfen","family":"Hua","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minjie","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruijing","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingchuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheyuan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongrui","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingtian","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,6,22]]},"reference":[{"key":"B1","author":"Ashurst","year":"2021","journal-title":"Klebsiella Pneumonia, StatPearls, StatPearls Publishing. 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