{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:30:45Z","timestamp":1792182645092,"version":"4.4.0"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T00:00:00Z","timestamp":1652054400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Surfactin lipopeptide is an eco-friendly microbially synthesized bioproduct that holds considerable potential in therapeutics (antibiofilm) as well as in agriculture (antifungal). In the present study, production of surfactin by a marine strain\n                    <jats:italic>Bacillus velezensis<\/jats:italic>\n                    MS20 was carried out, followed by physico-chemical characterization, anti-biofilm activity, plant growth promotion, and quantitative Reverse Transcriptase\u2014Polymerase Chain Reaction (q RT-PCR) studies. From the results, it was inferred that MS20 was found to produce biosurfactant (3,300 mg L\n                    <jats:sup>\u20131<\/jats:sup>\n                    ) under optimized conditions. From the physicochemical characterization [Thin layer chromatography (TLC), Fourier Transform Infrared (FTIR) Spectroscopy, Liquid Chromatography\/Mass Spectroscopy (LC\/MS), and Polymerase Chain Reaction (PCR) amplification] it was revealed to be surfactin. From bio-assay and scanning electron microscope (SEM) images, it was observed that surfactin (MIC 50 \u03bcg Ml\n                    <jats:sup>\u20131<\/jats:sup>\n                    ) has appreciable bacterial aggregation against clinical pathogens\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    MTCC424,\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    MTCC43,\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    MTCC9751, and Methicillin resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MRSA) and mycelial condensation property against a fungal phytopathogen\n                    <jats:italic>Rhizoctonia solani<\/jats:italic>\n                    . In addition, the q-RTPCR studies revealed 8-fold upregulation (9.34 \u00b1 0.11-fold) of\n                    <jats:italic>srf<\/jats:italic>\n                    A-A gene compared to controls. Further, treatment of maize crop (infected with\n                    <jats:italic>R. solani<\/jats:italic>\n                    ) with surfactin and MS20 led to the production of defense enzymes. In conclusion, concentration and synergy of a carbon source with inorganic\/mineral salts can ameliorate surfactin yield and, application wise, it has antibiofilm and antifungal activities. In addition, it induced systemic resistance in maize crop, which makes it a good candidate to be employed in sustainable agricultural practices.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2022.879739","type":"journal-article","created":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T12:18:55Z","timestamp":1652098735000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":78,"title":["Induction of Systemic Resistance in Maize and Antibiofilm Activity of Surfactin From Bacillus velezensis MS20"],"prefix":"10.3389","volume":"13","author":[{"given":"Shireen Adeeb Mujtaba","family":"Ali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Z.","family":"Sayyed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad I.","family":"Mir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Y.","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bee","family":"Hameeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustfa F.","family":"Alkhanani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shafiul","family":"Haque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdel Rahman","family":"Mohammad Al Tawaha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e9ter","family":"Poczai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,5,9]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1007\/s10532-018-09865-4","article-title":"Antibacterial, anti-adherent and cytotoxic activities of surfactin(s) from a lipolytic strain Bacillus safensis F4.","volume":"30","author":"Abdelli","year":"2019","journal-title":"Biodegradation"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23051049","article-title":"Culture Medium Development for Microbial-Derived Surfactants Production\u2014An Overview.","volume":"23","author":"Abdul","year":"2018","journal-title":"Molecules"},{"key":"B3","doi-asserted-by":"publisher","first-page":"1880","DOI":"10.1080\/10916460802686640","article-title":"Studies on the production of biosurfactant for the microbial enhanced oil recovery by using bacteria isolated from oil contaminated wet soil.","volume":"27","author":"Agarwal","year":"2009","journal-title":"Pet. Sci. Technol."},{"key":"B4","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/s10658-018-01639-1","article-title":"Efficiency of bacterial biosurfactant for biocontrol of Rhizoctonia solani (AG-4) causing root rot in faba bean (Vicia faba) plants.","volume":"153","author":"Akladious","year":"2019","journal-title":"Eur. J. Plant Pathol."},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0212447","article-title":"Design, antimicrobial activity and mechanism of action of Arg-rich ultra-short cationic lipopeptides.","volume":"14","author":"Armas","year":"2019","journal-title":"PLoS One"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10020209","article-title":"Antifungal Activity of Cyclic Tetrapeptide from Bacillus velezensis CE 100 against Plant Pathogen Colletotrichum gloeosporioides.","volume":"10","author":"Choub","year":"2021","journal-title":"Pathogens"},{"key":"B7","doi-asserted-by":"publisher","first-page":"1675","DOI":"10.1111\/j.1365-2672.2007.03701.x","article-title":"Antimicrobial potential of a lipopeptide biosurfactant derived from a marine Bacillus circulans.","volume":"104","author":"Das","year":"2008","journal-title":"J. Appl. Microbiol."},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbt.2015.02.009","article-title":"Sophorolipid biosurfactants: possible uses as antibacterial and antibiofilm agent.","volume":"32","author":"D\u00edaz De Rienzo","year":"2015","journal-title":"New Biotechnol"},{"key":"B9","doi-asserted-by":"publisher","first-page":"784","DOI":"10.4014\/jmb.1712.12014","article-title":"Exogenous Indole Regulates Lipopeptide Biosynthesis in Antarctic Bacillus amyloliquefaciens Pc3.","volume":"28","author":"Ding","year":"2018","journal-title":"J. Microbiol. Biotechnol"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0166079","article-title":"Bacillus methylotrophicus strain NKG-1, isolated from changbai mountain, china, has potential applications as a biofertilizer or biocontrol agent.","volume":"11","author":"Ge","year":"2016","journal-title":"PLoS One"},{"key":"B11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2017\/5893123","article-title":"Enhancement of Surfactin and Fengycin Production by Bacillus mojavensis A21: application for Diesel Biodegradation.","volume":"2017","author":"Hmidet","year":"2017","journal-title":"BioMed. Res. Internat"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1007\/s40011-015-0688-5","article-title":"Banded Leaf and Sheath Blight of Maize: historical Perspectives, Current Status and Future Directions.","volume":"87","author":"Hooda","year":"2017","journal-title":"Proc. Natl. Acad. Sci."},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26123488","article-title":"Sustainable Surfactin Production by Bacillus subtilis Using Crude Glycerol from Different Wastes.","volume":"26","author":"Janek","year":"2021","journal-title":"Molecules"},{"key":"B14","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1094\/MPMI-22-4-0456","article-title":"Insights into the Defense-Related Events Occurring in Plant Cells Following Perception of Surfactin-Type Lipopeptide from Bacillus subtilis.","volume":"22","author":"Jourdan","year":"2009","journal-title":"Am. Phytopathol. Soc."},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1080\/09603123.2019.1664729","article-title":"Surfactants of microbial origin as antibiofilm agents.","volume":"2019","author":"Katarzyna","year":"2019","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"B16","doi-asserted-by":"publisher","first-page":"091","DOI":"10.7324\/JAPS.2018.8313","article-title":"Lipopeptide antibiotic production by Bacillus velezensis KLP2016.","volume":"8","author":"Khem","year":"2018","journal-title":"J. Appl. Pharm. Sci."},{"key":"B17","doi-asserted-by":"crossref","first-page":"1254","DOI":"10.1520\/JFS13711J","article-title":"extraction strategies for amplified fragment length polymorphism analysis.","volume":"39","author":"Koons","year":"1994","journal-title":"J. Forensic."},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.561060","article-title":"Antimicrobial Activity of Lipopeptide Biosurfactants Against Foodborne Pathogen and Food Spoilage Microorganisms and Their Cytotoxicity.","volume":"11","author":"Kourmentza","year":"2021","journal-title":"Front. Microbiol."},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10091472","article-title":"Microbial Biosurfactant: a New Frontier for Sustainable Agriculture and Pharmaceutical Industries.","volume":"10","author":"Kumar","year":"2021","journal-title":"Antioxidants"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1186\/s12896-021-00690-x","article-title":"Evaluating the biocontrol potential of Canadian strain Bacillus velezensis 1B-23 via its surfactin production at various pHs and temperatures.","volume":"21","author":"Li","year":"2021","journal-title":"BMC Biotechnol"},{"key":"B21","doi-asserted-by":"publisher","first-page":"1540","DOI":"10.1038\/s41588-019-0503-y","article-title":"Natural variation in ZmFBL41 confers banded leaf and sheath blight resistance in maize.","volume":"51","author":"Li","year":"2019","journal-title":"Nat. Genet"},{"key":"B22","doi-asserted-by":"publisher","first-page":"488","DOI":"10.5423\/PPJ.OA.04.2017.0073","article-title":"Diffusible and volatile antifungal compounds produced by an antagonistic Bacillus velezensis G341 against various phytopathogenic fungi.","volume":"33","author":"Lim","year":"2017","journal-title":"Plant Pathol. J"},{"key":"B23","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1111\/jam.14459","article-title":"Antimicrobial Bacillus velezensis HC6: production of three kinds of lipopeptides and biocontrol potential in maize.","volume":"128","author":"Liu","year":"2020","journal-title":"J. Appl. Microbiol"},{"key":"B24","doi-asserted-by":"publisher","first-page":"5083","DOI":"10.1007\/s00253-014-6317-z","article-title":"Surfactin from Bacillus subtilis displays an unexpected anti-Legionella activity.","volume":"99","author":"Loiseau","year":"2015","journal-title":"Appl. Microbiol. Biotechnol"},{"key":"B25","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/j.biortech.2017.05.120","article-title":"Biosurfactant surfactin with pH-regulated emulsification activity for efficient oil separation when used as emulsifier.","volume":"241","author":"Long","year":"2017","journal-title":"Bioresour. Technol."},{"key":"B26","doi-asserted-by":"crossref","first-page":"2846","DOI":"10.3390\/md11082846","article-title":"Diversity of Secondary Metabolites from Marine Bacillus Species: chemistry and Biological Activity.","volume":"11","author":"Mondol","year":"2013","journal-title":"Mar Drugs"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2019.109372","article-title":"Enhancement of atrazine biodegradation by marine isolate Bacillus velezensis MHNK1 in presence of surfactin lipopeptide.","volume":"182","author":"Parameshwar","year":"2019","journal-title":"Ecotoxicol. Env. Safety"},{"key":"B28","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1128\/IAI.00877-12","article-title":"Tannic acid inhibits Staphylococcus aureus surface colonization in an IsaA-dependent manner.","volume":"81","author":"Payne","year":"2013","journal-title":"Infect Immun."},{"key":"B29","doi-asserted-by":"publisher","first-page":"69","DOI":"10.12691\/wjar-7-2-5","article-title":"Enhancement of Plant Growth in Tomato by Inoculation with Plant Growth Promoting Bacillus spp.","volume":"7","author":"Ramavath","year":"2019","journal-title":"World J. Agricult. Res."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.colsurfb.2010.10.045","article-title":"Antimicrobial and anti-adhesive potential of a biosurfactant Rufisan produced by Candida lipolytica UCP 0988.","volume":"84","author":"Rodrigues","year":"2011","journal-title":"Colloids Surf. B. Biointerf"},{"key":"B31","first-page":"256","author":"Sadasivam","year":"1996","journal-title":"Biochemical Methods."},{"key":"B32","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0198107","article-title":"Qualitative analysis of biosurfactants from Bacillus species exhibiting antifungal activity.","volume":"13","author":"Sarwar","year":"2018","journal-title":"PLoS One"},{"key":"B33","first-page":"119","article-title":"Surfactin \u2013 A Review.","volume":"48","author":"Shaligram","year":"2010","journal-title":"Food Technol. Biotechnol."},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph17041396","article-title":"Seed Biopriming with Microbial Inoculant Triggers Local and Systemic Defense Responses against Rhizoctonia solani Causing Banded Leaf and Sheath Blight in Maize (Zea mays L.).","volume":"17","author":"Singh","year":"2020","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"B35","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1002\/bit.20241","article-title":"Volumetric measurements of bacterial cells and extracellular polymeric substance glycoconjugates in biofilms.","volume":"88","author":"Staudt","year":"2004","journal-title":"Biotechnol. Bioeng"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9112251","article-title":"The Role of Surfactin Production by Bacillus velezensis on Colonization, Biofilm Formation on Tomato Root and Leaf Surfaces and Subsequent Protection (ISR) against Botrytis cinerea.","volume":"9","author":"Stoll","year":"2021","journal-title":"Microorganisms"},{"key":"B37","first-page":"432","article-title":"Bioreduction of Cr (VI) by biosurfactant producing marine bacterium Bacillus subtilis SHB 13.","volume":"75","author":"Swapna","year":"2016","journal-title":"J. Sci. Ind. Res."},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.6184","article-title":"Genomic Insights Into the Antifungal Activity and Plant Growth-Promoting Ability in Bacillus velezensis CMRP 4490.","volume":"11","author":"Teixeira","year":"2021","journal-title":"Front. Microbiol"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.623701","article-title":"The Surfactin-Like Lipopeptides From Bacillus spp.: Natural Biodiversity and Synthetic Biology for a Broader Application Range.","volume":"9","author":"Th\u00e9atre","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"key":"B40","first-page":"421","author":"Thimmaiah","year":"2012","journal-title":"Standard Methods of Biochemical Analysis."},{"key":"B41","doi-asserted-by":"publisher","first-page":"5095","DOI":"10.3390\/ijms11125095","article-title":"Rhamnolipid biosurfactants as new players in animal and plant defense against microbes.","volume":"11","author":"Vatsa","year":"2010","journal-title":"Int. J. Mol. Sci."},{"key":"B42","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.procbio.2006.07.025","article-title":"Using Taguchi experimental design methods to optimize trace element composition for enhanced surfactin production by Bacillus subtilis ATCC 21332.","volume":"42","author":"Wei","year":"2007","journal-title":"Proc. Biochem."},{"key":"B43","doi-asserted-by":"publisher","first-page":"120","DOI":"10.5454\/mi.9.3.4","article-title":"Optimization of Surfactin Production by Bacillus amyloliquefaciens MD4-12 Using Response Surface Methodology.","volume":"9","author":"Wibisana","year":"2015","journal-title":"Microbiol. Indon."},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.2686","article-title":"Bacterial Aggregation Assay in the Presence of Cyclic Lipopeptides.","volume":"8","author":"Xiu","year":"2018","journal-title":"Bio-protocol"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-015-1985-0","article-title":"\u03b2-1,3-1,4-glucanase gene from Bacillus velezensis ZJ20 exerts antifungal effect on plant pathogenic fungi.","volume":"32","author":"Xu","year":"2016","journal-title":"World J. Microbiol. Biotechnol"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-020-02034-9","article-title":"Rhamnolipids and surfactin inhibit the growth or formation of oral bacterial biofilm.","volume":"20","author":"Yamasaki","year":"2020","journal-title":"BMC Microbiol."},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0191991","article-title":"Cellulose-dependent expression and antibacterial characteristics of surfactin from Bacillus subtilis HH2 isolated from the giant panda.","volume":"13","author":"Zhou","year":"2018","journal-title":"PLoS One"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116935","article-title":"Transcriptional regulation and adaptation to a high-fiber environment in Bacillus subtilis HH2 isolated from feces of the Giant Panda.","volume":"10","author":"Zhou","year":"2015","journal-title":"PLoS One"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.879739\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:57:38Z","timestamp":1727139458000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.879739\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,9]]},"references-count":48,"alternative-id":["10.3389\/fmicb.2022.879739"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2022.879739","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,9]]},"article-number":"879739"}}