{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:04:28Z","timestamp":1792022668665,"version":"4.4.0"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T00:00:00Z","timestamp":1672272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Tongue diagnosis is a unique aspect of traditional Chinese medicine for diagnosing diseases before determining proper means of treatment, but it also has the disadvantage of relying on the subjective experience of medical practitioners and lack objective basis. The purpose of this article is to elucidate tongue-coating microbiota and metabolic differences in primary liver cancer (PLC) patients with thick or greasy tongue coatings. Tongue-coating samples were analyzed in 60 PLC patients (30 PLC with thick or greasy tongue-coating patients and 30 PLC with tongue-coating neither thick nor greasy) and 25 healthy controls (HC) using 16S rRNA gene sequencing technology. As compared to healthy individuals, tongue coatings of patients with PLC had elevated levels of <jats:italic>Firmicutes<\/jats:italic> and <jats:italic>Actinobacteria<\/jats:italic>. The abundance of <jats:italic>Fusobacteria, SR1_Absconditabacteria_<\/jats:italic>, and <jats:italic>Spirochaete<\/jats:italic> were higher in tongue coatings of healthy controls compared to samples in patients with PLC. In addition to site-specific differences, higher abundances of <jats:italic>Fusobacteria<\/jats:italic> and <jats:italic>Actinobacteria<\/jats:italic> were observed in thick or greasy tongue-coating patients as compared to non-thick and greasy tongue-coating patients. The inferred metagenomic pathways enriched in the PLC tongue-coating patients were mainly those involved in replication, recombination, and repair of protein. We also identify a tongue-coating microbiome signature to discriminate HC and PLC, including 15 variables on genus level. The prediction performance of the signature showed well in the training and validation cohorts. This research illustrates specific clinical features and bacterial structures in PLC patients with different tongue coatings, which facilitates understanding of the traditional tongue diagnosis.<\/jats:p>","DOI":"10.3389\/fmicb.2022.903616","type":"journal-article","created":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T11:38:18Z","timestamp":1672313898000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Relationship between thick or greasy tongue-coating microbiota and tongue diagnosis in patients with primary liver cancer"],"prefix":"10.3389","volume":"13","author":[{"given":"Yuren","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hetong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyu","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongbin","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shasha","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Zhai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,12,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"105271","DOI":"10.1016\/j.archoralbio.2021.105271","article-title":"Tongue-coating microbiome as a cancer predictor: a scoping review","volume":"132","author":"Ali Mohammed","year":"2021","journal-title":"Archives Oral Biol."},{"key":"B2","first-page":"18","article-title":"Understanding diagnostic reasoning in TCM practice: tongue diagnosis","volume":"15","author":"Anastasi","year":"2009","journal-title":"Alternat. 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