{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:32:17Z","timestamp":1787506337451,"version":"build-2736575974"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The purpose of this study was to elucidate the characteristics of the gut microbiome in patients with Polycystic ovary syndrome (PCOS) and analyze the alterations of fecal fatty acid metabolism, so as to further provide the pathogenesis of PCOS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Fecal samples from the PCOS group (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200931) and healthy control group (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200927) were analyzed by 16S rRNA gene sequencing and untargeted metabolomics. Peripheral venous blood was collected to measure serum inflammation and intestinal permeability. Finally, the correlation analysis of intestinal flora, fecal metabolites, and laboratory indicators was carried out.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Serum D-lactate content in the PCOS group was higher than that in the control group. There was no significant difference in microbial \u03b1 diversity and \u03b2 diversity between PCOS patients and healthy controls. Peptostreptococcaceae and Bacteroidales S24-7 group existed significant differences between PCOS patients and healthy controls. Based on linear discriminant analysis selection, 14 genera including\n                      <jats:italic>Klebsiella<\/jats:italic>\n                      ,\n                      <jats:italic>Enterobacteriaceae<\/jats:italic>\n                      , and\n                      <jats:italic>Gammaproteobacteria<\/jats:italic>\n                      were dominant in patients with PCOS, while 4 genera, including\n                      <jats:italic>rumenococcus<\/jats:italic>\n                      (\n                      <jats:italic>Ruminocaccaceae UCG 013<\/jats:italic>\n                      ),\n                      <jats:italic>prewortella<\/jats:italic>\n                      (\n                      <jats:italic>Prevotellaceae UCG 001<\/jats:italic>\n                      ), and\n                      <jats:italic>erysipelas<\/jats:italic>\n                      (\n                      <jats:italic>Erysipelatoclostridium<\/jats:italic>\n                      ), were dominant in healthy controls. Compared with PCOS with Body mass index (BMI)\u2009&amp;lt;\u200924, patients with BMI\u2009\u2265\u200924 have multiple dominant genera including\n                      <jats:italic>Abiotrophia<\/jats:italic>\n                      and\n                      <jats:italic>Peptostreptococcaceae<\/jats:italic>\n                      . Moreover, serum levels of free testosterone and androstenedione were positively correlated with\n                      <jats:italic>Megamonas<\/jats:italic>\n                      , while total testosterone was negatively correlated with\n                      <jats:italic>Alistipes<\/jats:italic>\n                      . Additionally, fecal contents of acetic acid and propionic acid in patients with PCOS were significantly higher than those in healthy controls.\n                      <jats:italic>Eubacterium_coprostanoligenes_group<\/jats:italic>\n                      and\n                      <jats:italic>Alistipes<\/jats:italic>\n                      were positively correlated with 6 kinds of fatty acids.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Specific intestinal flora fecal fatty acids and serum metabolites may mediate the occurrence and development of PCOS. PCOS patients with different body sizes have specific intestinal flora.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2022.911992","type":"journal-article","created":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T22:59:51Z","timestamp":1656629991000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":36,"title":["Alterations of Gut Microbiome and Fecal Fatty Acids in Patients With Polycystic Ovary Syndrome in Central China"],"prefix":"10.3389","volume":"13","author":[{"given":"Gailing","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenguo","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huirong","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xunjie","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guijun","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,7,1]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"e0226372","DOI":"10.1371\/journal.pone.0226372","article-title":"Analysis of gut microbiota of obese individuals with type 2 diabetes and healthy individuals","volume":"14","author":"Ahmad","year":"2019","journal-title":"PLoS One"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"16057","DOI":"10.1038\/nrdp.2016.57","article-title":"Polycystic ovary syndrome","volume":"2","author":"Azziz","year":"2016","journal-title":"Nat. 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