{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:22:25Z","timestamp":1792020145426,"version":"4.4.0"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,6,13]],"date-time":"2022-06-13T00:00:00Z","timestamp":1655078400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32000139"],"award-info":[{"award-number":["32000139"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31970178"],"award-info":[{"award-number":["31970178"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006190","name":"Research and Development","doi-asserted-by":"publisher","award":["2019YFC1200500"],"award-info":[{"award-number":["2019YFC1200500"]}],"id":[{"id":"10.13039\/100006190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p><jats:italic>Chlamydia psittaci<\/jats:italic> is the causative agent of psittacosis, a worldwide zoonotic disease. A rapid, specific, and sensitive diagnostic assay would be benefit for <jats:italic>C. psittaci<\/jats:italic> infection control. In this study, an assay combining recombinase-aided amplification and a lateral flow strip (RAA-LF) for the detection of active <jats:italic>C. psittaci<\/jats:italic> infection was developed. The RAA-LF assay targeted the <jats:italic>CPSIT_RS02830<\/jats:italic> gene of <jats:italic>C. psittaci<\/jats:italic> and could be accomplished in 15\u2009min at a single temperature (39\u00b0C). The analytical sensitivity of the assay was as low as 1\u2009\u00d7\u200910<jats:sup>0<\/jats:sup> copies\/\u03bcl and no cross-reaction with some other intracellular pathogens was observed. Moreover, all feces samples from mice infected with <jats:italic>C. psittaci<\/jats:italic> at day-1 post-infection were positive in the RAA-LF assay. In conclusion, the RAA-LF assay provides a convenient, rapid, specific and sensitive method for detection of active <jats:italic>C. psittaci<\/jats:italic> infection and it is also suitable for <jats:italic>C. psittaci<\/jats:italic> detection in field.<\/jats:p>","DOI":"10.3389\/fmicb.2022.928025","type":"journal-article","created":{"date-parts":[[2022,6,13]],"date-time":"2022-06-13T05:58:28Z","timestamp":1655099908000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active Chlamydia psittaci Infection"],"prefix":"10.3389","volume":"13","author":[{"given":"Jun","family":"Jiao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peisheng","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiqiang","family":"Wan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"OuYang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghui","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bohai","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolu","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,6,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"115","DOI":"10.3389\/fmicb.2017.00115","article-title":"PCR inhibition of a quantitative PCR for detection of Mycobacterium avium subspecies Paratuberculosis DNA in feces: diagnostic implications and potential solutions","volume":"8","author":"Acharya","year":"2017","journal-title":"Front. 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