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Microbiol."],"abstract":"<jats:p>Members of the genus <jats:italic>Pseudomonas<\/jats:italic> are known to be widespread in hydrocarbon contaminated environments because of their remarkable ability to degrade a variety of petroleum hydrocarbons, including BTEX (benzene, toluene, ethylbenzene and xylene) compounds. During an enrichment investigation which aimed to study microaerobic xylene degradation in a legacy petroleum hydrocarbon-contaminated groundwater, a novel Gram-stain-negative, aerobic, motile and rod-shaped bacterial strain, designated as MAP12<jats:sup>T<\/jats:sup> was isolated. It was capable of degrading benzene, toluene, <jats:italic>meta<\/jats:italic>- and <jats:italic>para<\/jats:italic>- xylene effectively under both aerobic and microaerobic conditions. The 16S rRNA gene sequence analysis revealed that strain MAP12<jats:sup>T<\/jats:sup> belongs to the genus <jats:italic>Pseudomonas<\/jats:italic>, with the highest 16S rRNA gene similarity to <jats:italic>Pseudomonas linyingensis<\/jats:italic> LYBRD3-7<jats:italic><jats:sup>T<\/jats:sup><\/jats:italic> (98.42%), followed by <jats:italic>Pseudomonas sagittaria<\/jats:italic> JCM 18195<jats:italic><jats:sup>T<\/jats:sup><\/jats:italic> (98.29%) and <jats:italic>Pseudomonas alcaliphila<\/jats:italic> JCM 10630<jats:italic><jats:sup>T<\/jats:sup><\/jats:italic> (98.08%). Phylogenomic tree constructed using a concatenated alignment of 92 core genes indicated that strain MAP12<jats:sup>T<\/jats:sup> is distinct from any known <jats:italic>Pseudomonas<\/jats:italic> species. The draft genome sequence of strain MAP12<jats:sup>T<\/jats:sup> is 4.36 Mb long, and the G+C content of MAP12<jats:sup>T<\/jats:sup> genome is 65.8%. Orthologous average nucleotide identity (OrthoANI) and digital DNA\u2013DNA hybridization (dDDH) analyses confirmed that strain MAP12<jats:sup>T<\/jats:sup> is distinctly separated from its closest neighbors (OrthoANI &amp;lt; 89 %; dDDH &amp;lt; 36%). Though several members of the genus <jats:italic>Pseudomonas<\/jats:italic> are well known for their aerobic BTEX degradation capability, this is the first report of a novel <jats:italic>Pseudomonas<\/jats:italic> species capable of degrading xylene under microaerobic conditions. By applying genome-resolved metagenomics, we were able to partially reconstruct the genome of strain MAP12<jats:italic><jats:sup>T<\/jats:sup><\/jats:italic> from metagenomics sequence data and showed that strain MAP12<jats:italic><jats:sup>T<\/jats:sup><\/jats:italic> was an abundant member of the xylene-degrading bacterial community under microaerobic conditions. Strain MAP12<jats:sup>T<\/jats:sup> contains ubiquinone 9 (Q9) as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine as major polar lipids. The major cellular fatty acids of strain MAP12<jats:sup>T<\/jats:sup> are summed feature 3 (C<jats:sub>16:1<\/jats:sub>\u03c96c and\/or C<jats:sub>16:1<\/jats:sub>\u03c97c), C<jats:sub>16:0<\/jats:sub> and summed feature 8 (C<jats:sub>18:1<\/jats:sub>\u03c96c and\/or C<jats:sub>18:1<\/jats:sub>\u03c97c). The results of this polyphasic study support that strain MAP12<jats:sup>T<\/jats:sup> represents a novel species of the genus <jats:italic>Pseudomonas<\/jats:italic>, hence the name of <jats:italic>Pseudomonas aromaticivorans<\/jats:italic> sp. nov. is proposed for this strain considering its aromatic hydrocarbon degradation capability. The type strain is MAP12<jats:sup>T<\/jats:sup> (=LMG 32466, =NCAIM B.02668).<\/jats:p>","DOI":"10.3389\/fmicb.2022.929128","type":"journal-article","created":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T04:25:06Z","timestamp":1663647906000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":36,"title":["Isolation of Pseudomonas aromaticivorans sp. nov from a hydrocarbon-contaminated groundwater capable of degrading benzene-, toluene-, m- and p-xylene under microaerobic conditions"],"prefix":"10.3389","volume":"13","author":[{"given":"Sinchan","family":"Banerjee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Bedics","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"T\u00f3th","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bal\u00e1zs","family":"Kriszt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 R.","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K\u00e1roly","family":"B\u00f3ka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e1s","family":"T\u00e1ncsics","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"28431","DOI":"10.1007\/s11356-021-18300-w","article-title":"Evaluating the aerobic xylene-degrading potential of the intrinsic microbial community of a legacy BTEX-contaminated aquifer by enrichment culturing coupled with multi-omics analysis: uncovering the role of Hydrogenophaga strains in xylene degradation.","volume":"29","author":"Banerjee","year":"2022","journal-title":"Environ. 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