{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:42:10Z","timestamp":1790707330800,"version":"4.1.0"},"reference-count":81,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T00:00:00Z","timestamp":1664150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Polychlorinated biphenyls (PCBs) are persistent organic pollutants widely distributed in the environment and possess deleterious health effects. The main objective of the study was to obtain bacterial isolates from PCB-contaminated soil for enhanced biodegradation of PCB-77. Selective enrichment resulted in the isolation of 33 strains of PCB-contaminated soil nearby Bhilai steel plant, Chhattisgarh, India. Based on the prominent growth using biphenyl as the sole carbon source and the confirmation of its degradation by GC-MS\/MS analysis, four isolates were selected for further study. The isolates identified by 16S rRNA gene sequencing were <jats:italic>Pseudomonas aeruginosa<\/jats:italic> MAPB-2, <jats:italic>Pseudomonas plecoglossicida<\/jats:italic> MAPB-6, <jats:italic>Brucella anthropi<\/jats:italic> MAPB-9, and <jats:italic>Priestia megaterium<\/jats:italic> MAPB-27. The isolate MAPB-9 showed a degradation of 66.15% biphenyl, while MAPB-2, MAPB-6, and MAPB-27 showed a degradation of 62.06, 57.02, and 56.55%, respectively in 48 h. Additionally, the degradation ability of these strains was enhanced with addition of co-metabolite glucose (0.2%) in the culture medium. Addition of glucose showed 100% degradation of biphenyl by MAPB-9, in 48 h, while MAPB-6, MAPB-2, and MAPB-27 showed 97.1, 67.5, and 53.3% degradation, respectively as analyzed by GC-MS\/MS. Furthermore, in the presence of inducer, PCB-77 was found to be 59.89, 30.49, 27.19, and 4.43% degraded by MAPB-6, MAPB-9, MAPB-2, and MAPB-27, respectively in 7 d. The production of biosurfactants that aid in biodegradation process were observed in all the isolates. This was confirmed by ATR-FTIR analysis that showed the presence of major functional groups (CH<jats:sub>2<\/jats:sub>, CH<jats:sub>3<\/jats:sub>, CH, = CH<jats:sub>2<\/jats:sub>, C\u2013O\u2013C, C-O) of the biosurfactant. The biosurfactants were further identified by HPTLC and GC-MS\/MS analysis. Present study is the first to report PCB-77 degradation potential of <jats:italic>Pseudomonas aeruginosa, B. anthropi, Pseudomonas plecoglossicida<\/jats:italic>, and <jats:italic>Priestia megaterium<\/jats:italic>. Similarly, this is the first report on <jats:italic>Pseudomonas plecoglossicida<\/jats:italic> and <jats:italic>Priestia megaterium<\/jats:italic> for PCB biodegradation. Our results suggest that the above isolates can be used for the biodegradation of biphenyl and PCB-77 in PCB-contaminated soil.<\/jats:p>","DOI":"10.3389\/fmicb.2022.952374","type":"journal-article","created":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T09:36:28Z","timestamp":1664184988000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":30,"title":["PCB-77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soil"],"prefix":"10.3389","volume":"13","author":[{"given":"Monika","family":"Sandhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atish T.","family":"Paul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaros\u0142aw","family":"Pro\u0107k\u00f3w","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Manuel P\u00e9rez","family":"de la Lastra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prabhat N.","family":"Jha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,26]]},"reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"241","DOI":"10.3109\/07388559009038210","article-title":"Aerobic and anaerobic biodegradation of PCBs: A review.","volume":"10","author":"Abramowicz","year":"1990","journal-title":"Crit. 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