{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:30:58Z","timestamp":1787499058457,"version":"build-2736575974"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:00:00Z","timestamp":1661904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    <jats:italic>Sophora alopecuroides<\/jats:italic>\n                    L. is a traditional Chinese medicine used for the treatment of several different disease states including bacillary dysentery and enteritis. But importantly, it also plays a role as an anti-tumor agent. That said, little is known about the role endophytes play regarding the clinically bioactive metabolites in\n                    <jats:italic>S. alopecuroides<\/jats:italic>\n                    . In order to explore the effects of endophytic fungi on the accumulation, quality, and correlation in the content of the medicinal compounds, the structural diversity of endophytic fungi in\n                    <jats:italic>S. alopecuroides<\/jats:italic>\n                    was analyzed. The relationship between endophytes and quinolizidine alkaloids (QAs), housed within the seeds of\n                    <jats:italic>S. alopecuroides<\/jats:italic>\n                    , which were interpreted based on established methods of high-throughput sequencing and high-performance liquid chromatography. A total of 1,034,418 effective sequence reads and 257 operational taxonomic units (OTUs) were obtained from 33 samples which were sourced from 11 different sampling sites and further classified into 9 phyla, 20 classes, 45 orders, 85 families, and 118 genera. Ascomycota was found to be the dominant phylum of endophytic fungi in\n                    <jats:italic>S. alopecuroides<\/jats:italic>\n                    , with a relative abundance ranging from 60.85 to 98.30%.\n                    <jats:italic>Alternaria<\/jats:italic>\n                    ,\n                    <jats:italic>Cladosporium<\/jats:italic>\n                    ,\n                    <jats:italic>Filobasidium<\/jats:italic>\n                    , and an unidentified Ascomycota were the core-shared endophytes, accounting for 49.96, 27.12, 14.83, and 7.88%, respectively. Correlation analysis showed that the Simpson\u2019s diversity index of endophytic fungal community in\n                    <jats:italic>S. alopecuroides<\/jats:italic>\n                    was significantly positively correlated with the Oxymatrine (OMA) content in different areas, while the Chao and Shannoneven indexes were significantly negatively correlated with OMA. The endophytic fungi of\n                    <jats:italic>Alternaria<\/jats:italic>\n                    were positively correlated with the content of OMA, Oxysophocarpine (OSC), and total QAs. This study has mastered the endophytic fungi resources of\n                    <jats:italic>S. alopecuroides<\/jats:italic>\n                    , explored potential functional endophytic fungi, and provided a scientific basis for using biological fertilization strategies to improve the quality of\n                    <jats:italic>S. alopecuroides<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2022.955647","type":"journal-article","created":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T05:15:48Z","timestamp":1662009348000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":23,"title":["Correlation in endophytic fungi community diversity and bioactive compounds of Sophora alopecuroides"],"prefix":"10.3389","volume":"13","author":[{"given":"Mingxiu","family":"Ju","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingchen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruotong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengnan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiwen","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,8,31]]},"reference":[{"key":"B1","article-title":"Towards a unified paradigm for verification and validation of systems engineering design models","author":"Alawneh","year":"2006","journal-title":"Proceedings of the IASTED International Conference on Software Engineering"},{"key":"B2","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1038\/nmeth.2276","article-title":"Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing.","volume":"10","author":"Bokulich","year":"2013","journal-title":"Nat. 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