{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T18:52:40Z","timestamp":1776538360071,"version":"3.51.2"},"reference-count":68,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,29]],"date-time":"2022-09-29T00:00:00Z","timestamp":1664409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Gausemycin A is the first member of the novel lipoglycopeptides family produced by <jats:italic>Streptomyces roseoflavus<\/jats:italic> INA-Ac-5812. Gausemycin A has a pronounced bactericidal activity against methicillin-resistant <jats:italic>Staphylococcus aureus<\/jats:italic>. However, the ability of <jats:italic>S. aureus<\/jats:italic> to be resistant to gausemycin A has not been investigated yet. Using serial passaging, we have obtained the resistant variant <jats:italic>S. aureus<\/jats:italic> 5812R, which is 80 times more resistant compared to the parent strain. Susceptibility testing of <jats:italic>S. aureus<\/jats:italic> 5812R revealed the acquisition of cross-resistance to daptomycin, cefazolin, tetracycline, and gentamicin, while the resistance to vancomycin, nisin, and ramoplanin was absent. Whole genome sequencing revealed single nucleotide polymorphism (SNP) and deletions in <jats:italic>S. aureus<\/jats:italic> 5812R, among which are genes encoding efflux pump (<jats:italic>sepA<\/jats:italic>), the two-component Kdp system (<jats:italic>kdpE<\/jats:italic>), and the component of isoprenoid biosynthesis pathway (<jats:italic>hepT)<\/jats:italic>. Phenotypically, <jats:italic>S. aureus<\/jats:italic> 5812R resembles a small-colony variant, as it is slow-growing, forms small colonies, and is deficient in pigments. Profiling of fatty acids (FA) composition constituting the cytoplasmic membrane of <jats:italic>S. aureus<\/jats:italic> 5812R revealed the prevalence of <jats:italic>anteiso<\/jats:italic>-branched FA, while straight FA was slightly less present. The evidence also showed that the gausemycin A-resistant strain has increased expression of the <jats:italic>cls2<\/jats:italic> gene of the cardiolipin synthase. The performed checkerboard assay pointed out that the combination of gausemycin A and ciprofloxacin showed a synergistic effect against <jats:italic>S. aureus<\/jats:italic> 5812R.<\/jats:p>","DOI":"10.3389\/fmicb.2022.963979","type":"journal-article","created":{"date-parts":[[2022,9,29]],"date-time":"2022-09-29T13:50:26Z","timestamp":1664459426000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Staphylococcus aureus is able to generate resistance to novel lipoglycopeptide antibiotic gausemycin A"],"prefix":"10.3389","volume":"13","author":[{"given":"Darya V.","family":"Poshvina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana S.","family":"Dilbaryan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey P.","family":"Kasyanov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera S.","family":"Sadykova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olda A.","family":"Lapchinskaya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugene A.","family":"Rogozhin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexey S.","family":"Vasilchenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1618","DOI":"10.1111\/j.1462-5822.2011.01645.x","article-title":"The two-component sensor kinase KdpD is required for salmonella typhimurium colonization of caenorhabditis elegans and survival in macrophages","volume":"13","author":"Alegado","year":"2011","journal-title":"Cell. 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