{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T06:35:58Z","timestamp":1776839758243,"version":"3.51.2"},"reference-count":126,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T00:00:00Z","timestamp":1660694400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000774","name":"Defense Threat Reduction Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000774","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p><jats:italic>Burkholderia pseudomallei<\/jats:italic>and the closely related species,<jats:italic>Burkholderia mallei<\/jats:italic>, produce similar multifaceted diseases which range from rapidly fatal to protracted and chronic, and are a major cause of mortality in endemic regions. Besides causing natural infections, both microbes are Tier 1 potential biothreat agents. Antibiotic treatment is prolonged with variable results, hence effective vaccines are urgently needed. The purpose of our studies was to compare candidate vaccines that target both melioidosis and glanders to identify the most efficacious one(s) and define residual requirements for their transition to the non-human primate aerosol model. Studies were conducted in the C57BL\/6 mouse model to evaluate the humoral and cell-mediated immune response and protective efficacy of three<jats:italic>Burkholderia<\/jats:italic>vaccine candidates against lethal aerosol challenges with<jats:italic>B. pseudomallei<\/jats:italic>K96243,<jats:italic>B. pseudomallei<\/jats:italic>MSHR5855, and<jats:italic>B. mallei<\/jats:italic>FMH. The recombinant vaccines generated significant immune responses to the vaccine antigens, and the live attenuated vaccine generated a greater immune response to OPS and the whole bacterial cells. Regardless of the candidate vaccine evaluated, the protection of mice was associated with a dampened cytokine response within the lungs after exposure to aerosolized bacteria. Despite being delivered by two different platforms and generating distinct immune responses, two experimental vaccines, a capsule conjugate + Hcp1 subunit vaccine and the live<jats:italic>B. pseudomallei<\/jats:italic>668 \u0394<jats:italic>ilvI<\/jats:italic>strain, provided significant protection and were down-selected for further investigation and advanced development.<\/jats:p>","DOI":"10.3389\/fmicb.2022.965518","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T08:39:16Z","timestamp":1660725556000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["Evaluation of two different vaccine platforms for immunization against melioidosis and glanders"],"prefix":"10.3389","volume":"13","author":[{"given":"Sergei S.","family":"Biryukov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher K.","family":"Cote","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher P.","family":"Klimko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer L.","family":"Dankmeyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathaniel O.","family":"Rill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer L.","family":"Shoe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa","family":"Hunter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zain","family":"Shamsuddin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"Velez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zander M.","family":"Hedrick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raysa","family":"Rosario-Acevedo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuli","family":"Talyansky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lindsey K.","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caitlyn E.","family":"Orne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David P.","family":"Fetterer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary N.","family":"Burtnick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul J.","family":"Brett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan L.","family":"Welkos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"DeShazer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,8,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1160","DOI":"10.3389\/fimmu.2020.01160","article-title":"IL-22 Plays a critical role in maintaining epithelial integrity during pulmonary infection","volume":"11","author":"Alcorn","year":"2020","journal-title":"Front. Immunol"},{"key":"B2","doi-asserted-by":"publisher","first-page":"196","DOI":"10.3390\/vaccines7040196","article-title":"Deletion of two genes in Burkholderia pseudomallei MSHR668 that target essential amino acids protect acutely infected BALB\/c mice and promote long term survival","volume":"7","author":"Amemiya","year":"2019","journal-title":"Vaccines"},{"key":"B3","doi-asserted-by":"publisher","first-page":"5290","DOI":"10.1128\/IAI.70.9.5290-5294.2002","article-title":"A mutant of Burkholderia pseudomallei, auxotrophic in the branched chain amino acid biosynthetic pathway, is attenuated and protective in a murine model of melioidosis","volume":"70","author":"Atkins","year":"2002","journal-title":"Infect. Immun"},{"key":"B4","doi-asserted-by":"publisher","first-page":"e35386","DOI":"10.1371\/journal.pone.0035386","article-title":"Polysaccharide specific monoclonal antibodies provide passive protection against intranasal challenge with Burkholderia pseudomallei","volume":"7","author":"AuCoin","year":"2012","journal-title":"PLoS ONE"},{"key":"B5","doi-asserted-by":"publisher","first-page":"49","DOI":"10.3390\/vaccines5040049","article-title":"A Burkholderia pseudomallei outer membrane vesicle vaccine provides cross protection against inhalational glanders in mice and non-human primates","volume":"5","author":"Baker","year":"2017","journal-title":"Vaccines"},{"key":"B6","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1038\/s41541-021-00281-z","article-title":"Burkholderia pseudomallei OMVs derived from infection mimicking conditions elicit similar protection to a live-attenuated vaccine","volume":"6","author":"Baker","year":"2021","journal-title":"NPJ Vaccines"},{"key":"B7","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.cyto.2015.05.008","article-title":"The differential expression of IL-4 and IL-13 and its impact on type-2 immunity","volume":"75","author":"Bao","year":"2015","journal-title":"Cytokine"},{"key":"B8","doi-asserted-by":"publisher","first-page":"e0172627","DOI":"10.1371\/journal.pone.0172627","article-title":"Characterization of pathogenesis of and immune response to Burkholderia pseudomallei K96243 using both inhalational and intraperitoneal infection models in BALB\/c and C57BL\/6 mice","volume":"12","author":"Bearss","year":"2017","journal-title":"PLoS ONE"},{"key":"B9","doi-asserted-by":"publisher","first-page":"2042","DOI":"10.1002\/eji.201445303","article-title":"IL-3 synergises with basophil-derived IL-4 and IL-13 to promote the alternative activation of human monocytes","volume":"45","author":"Borriello","year":"2015","journal-title":"Eur. J. Immunol"},{"key":"B10","doi-asserted-by":"publisher","first-page":"21","DOI":"10.3389\/fcimb.2016.00021","article-title":"Phenotypic characterization of a novel virulence-factor deletion strain of Burkholderia mallei that provides partial protection against inhalational glanders in mice","volume":"6","author":"Bozue","year":"2016","journal-title":"Front. Cell Infect. Microbiol"},{"key":"B11","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1002\/embr.201337936","article-title":"Type VI secretion and bacteriophage tail tubes share a common assembly pathway","volume":"15","author":"Brunet","year":"2014","journal-title":"EMBO Rep"},{"key":"B12","doi-asserted-by":"publisher","first-page":"1512","DOI":"10.1128\/IAI.01218-10","article-title":"The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei","volume":"79","author":"Burtnick","year":"2011","journal-title":"Infect. Immun"},{"key":"B13","doi-asserted-by":"publisher","first-page":"108","DOI":"10.3389\/fcimb.2012.00108","article-title":"Development of capsular polysaccharide-based glycoconjugates for immunization against melioidosis and glanders","volume":"2","author":"Burtnick","year":"","journal-title":"Front. Cell Infect. Microbiol"},{"key":"B14","doi-asserted-by":"publisher","first-page":"148","DOI":"10.3389\/fcimb.2012.00148","article-title":"Development of novel O-polysaccharide based glycoconjugates for immunization against glanders","volume":"2","author":"Burtnick","year":"","journal-title":"Front. Cell Infect. Microbiol"},{"key":"B15","doi-asserted-by":"publisher","first-page":"e00724","DOI":"10.1128\/IAI.00724-17","article-title":"Development of subunit vaccines that provide high-level protection and sterilizing immunity against acute inhalational melioidosis","volume":"86","author":"Burtnick","year":"2018","journal-title":"Infect. Immun"},{"key":"B16","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1016\/j.etp.2010.11.011","article-title":"Protective effect of Ulinastatin against murine models of sepsis: inhibition of TNF-alpha and IL-6 and augmentation of IL-10 and IL-13","volume":"64","author":"Cao","year":"2012","journal-title":"Exp. Toxicol. Pathol"},{"key":"B17","doi-asserted-by":"publisher","first-page":"2616","DOI":"10.1016\/j.vaccine.2016.03.088","article-title":"Identification of an OmpW homologue in Burkholderia pseudomallei, a protective vaccine antigen against melioidosis","volume":"34","author":"Casey","year":"2016","journal-title":"Vaccine"},{"key":"B18","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1177\/039463201002300304","article-title":"IL-31 a Th2 cytokine involved in immunity and inflammation","volume":"23","author":"Castellani","year":"2010","journal-title":"Int. J. Immunopathol. Pharmacol"},{"key":"B19","doi-asserted-by":"publisher","first-page":"e1002452","DOI":"10.1371\/journal.ppat.1002452","article-title":"Inflammasome-dependent pyroptosis and IL-18 protect against Burkholderia pseudomallei lung infection while IL-1\u03b2 is deleterious","volume":"7","author":"Ceballos-Olvera","year":"2011","journal-title":"PLoS Pathog"},{"key":"B20","doi-asserted-by":"publisher","first-page":"463","DOI":"10.3201\/eid2702.200213","article-title":"Role of Burkholderia pseudomallei-specific IgG2 in adults with acute melioidosis, Thailand","volume":"27","author":"Chaichana","year":"2021","journal-title":"Emerg. Infect. Dis"},{"key":"B21","doi-asserted-by":"publisher","first-page":"E1","DOI":"10.2807\/esm.10.03.00528-en","article-title":"Bioterrorism, glanders and melioidosis","volume":"10","author":"Cheng","year":"2005","journal-title":"Euro. Surveill."},{"key":"B22","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1099\/0022-1317-50-1-55","article-title":"Distribution of immunoglobulin classes and IgG subclasses against a culture filtrate antigen of Burkholderia pseudomallei in melioidosis patients","volume":"50","author":"Chenthamarakshan","year":"2001","journal-title":"J. Med. Microbiol"},{"key":"B23","doi-asserted-by":"publisher","first-page":"85","DOI":"10.3389\/fcimb.2012.00085","article-title":"Immunogenic recombinant Burkholderia pseudomallei MprA serine protease elicits protective immunity in mice","volume":"2","author":"Chin","year":"2012","journal-title":"Front. Cell Infect. Microbiol"},{"key":"B24","doi-asserted-by":"publisher","first-page":"16","DOI":"10.3390\/vaccines9020161","article-title":"Protection elicited by attenuated live yersinia pestis vaccine strains against lethal infection with virulent Y. pestis","volume":"9","author":"Cote","year":"2021","journal-title":"Vaccines"},{"key":"B25","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1055\/s-0034-1398389","article-title":"Melioidosis: evolving concepts in epidemiology, pathogenesis, and treatment","volume":"36","author":"Currie","year":"2015","journal-title":"Semin. Respir. Crit. Care Med"},{"key":"B26","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1097\/00000658-199204000-00009","article-title":"Cytokine serum level during severe sepsis in human IL-6 as a marker of severity","volume":"215","author":"Damas","year":"1992","journal-title":"Ann. Surg"},{"key":"B27","doi-asserted-by":"publisher","first-page":"13","DOI":"10.3390\/tropicalmed3010013","article-title":"Global burden and challenges of melioidosis","volume":"3","author":"Dance","year":"2018","journal-title":"Trop. Med. Infect. Dis"},{"key":"B28","doi-asserted-by":"publisher","first-page":"2095","DOI":"10.1182\/blood.V87.6.2095.bloodjournal8762095","article-title":"Biologic basis for interleukin-1 in disease","volume":"87","author":"Dinarello","year":"1996","journal-title":"Blood"},{"key":"B29","first-page":"483","article-title":"Interleukin-18, a proinflammatory cytokine","volume":"11","author":"Dinarello","year":"2000","journal-title":"Eur. Cytokine Netw"},{"key":"B30","doi-asserted-by":"publisher","first-page":"12143","DOI":"10.1038\/s41598-017-12331-5","article-title":"Infection with Burkholderia pseudomallei- immune correlates of survival in acute melioidosis","volume":"7","author":"Dunachie","year":"2017","journal-title":"Sci. Rep"},{"key":"B31","doi-asserted-by":"publisher","first-page":"636","DOI":"10.1093\/infdis\/jir301","article-title":"Combining vaccination and postexposure CpG therapy provides optimal protection against lethal sepsis in a biodefense model of human melioidosis","volume":"204","author":"Easton","year":"2011","journal-title":"J. Infect. Dis"},{"key":"B32","doi-asserted-by":"publisher","first-page":"13499","DOI":"10.1073\/pnas.0812080106","article-title":"A Burkholderia pseudomallei protein microarray reveals serodiagnostic and cross-reactive antigens","volume":"106","author":"Felgner","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"B33","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1093\/intimm\/9.4.467","article-title":"Effects of IL-13 on murine listeriosis","volume":"9","author":"Flesch","year":"1997","journal-title":"Int. Immunol"},{"key":"B34","doi-asserted-by":"publisher","first-page":"2867","DOI":"10.1016\/S0264-410X(00)00076-1","article-title":"A genetically-defined riboflavin auxotroph of Actinobacillus pleuropneumoniae as a live attenuated vaccine","volume":"18","author":"Fuller","year":"2000","journal-title":"Vaccine"},{"key":"B35","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1146\/annurev.micro.112408.134030","article-title":"Molecular insights into Burkholderia pseudomallei and Burkholderia mallei pathogenesis","volume":"64","author":"Galyov","year":"2010","journal-title":"Annu. Rev. Microbiol"},{"key":"B36","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1016\/j.nano.2014.08.005","article-title":"A gold nanoparticle-linked glycoconjugate vaccine against Burkholderia mallei","volume":"11","author":"Gregory","year":"2015","journal-title":"Nanomedicine"},{"key":"B37","doi-asserted-by":"publisher","first-page":"10566","DOI":"10.1038\/s41598-017-11127-x","article-title":"Immunization with alkyl hydroperoxide reductase subunit C reduces Fusobacterium nucleatum load in the intestinal tract","volume":"7","author":"Guo","year":"2017","journal-title":"Sci. Rep"},{"key":"B38","doi-asserted-by":"publisher","first-page":"4173","DOI":"10.1128\/IAI.00404-07","article-title":"Identification of a LolC homologue in Burkholderia pseudomallei, a novel protective antigen for melioidosis","volume":"75","author":"Harland","year":"2007","journal-title":"Infect. Immun"},{"key":"B39","doi-asserted-by":"publisher","first-page":"2345","DOI":"10.1128\/IAI.00328-16","article-title":"Burkholderia mallei CLH001 attenuated vaccine strain is immunogenic and protects against acute respiratory glanders","volume":"84","author":"Hatcher","year":"2016","journal-title":"Infect. Immun"},{"key":"B40","doi-asserted-by":"publisher","first-page":"e36507","DOI":"10.1371\/journal.pone.0036507","article-title":"Evolution of Burkholderia pseudomallei in recurrent melioidosis","volume":"7","author":"Hayden","year":"2012","journal-title":"PLoS ONE"},{"key":"B41","doi-asserted-by":"publisher","first-page":"5945","DOI":"10.1128\/IAI.73.9.5945-5951.2005","article-title":"Humoral and cell-mediated adaptive immune responses are required for protection against Burkholderia pseudomallei challenge and bacterial clearance postinfection","volume":"73","author":"Healey","year":"2005","journal-title":"Infect. Immun"},{"key":"B42","doi-asserted-by":"publisher","first-page":"5304","DOI":"10.1016\/j.vaccine.2011.05.009","article-title":"Mucosal immunization with liposome-nucleic acid adjuvants generates effective humoral and cellular immunity","volume":"29","author":"Henderson","year":"2011","journal-title":"Vaccine"},{"key":"B43","doi-asserted-by":"publisher","first-page":"e2727","DOI":"10.1371\/journal.pntd.0002727","article-title":"Development of a prototype lateral flow immunoassay (LFI) for the rapid diagnosis of melioidosis","volume":"8","author":"Houghton","year":"2014","journal-title":"PLoS Negl. Trop. Dis"},{"key":"B44","doi-asserted-by":"publisher","first-page":"1376","DOI":"10.4049\/jimmunol.2000454","article-title":"B Lymphocyte-derived CCL7 augments neutrophil and monocyte recruitment, exacerbating acute kidney injury","volume":"205","author":"Inaba","year":"2020","journal-title":"J. Immunol"},{"key":"B45","doi-asserted-by":"crossref","first-page":"2446","DOI":"10.4049\/jimmunol.148.8.2446","article-title":"MHC interaction and T cell recognition of carbohydrates and glycopeptides","volume":"148","author":"Ishioka","year":"1992","journal-title":"J. Immunol"},{"key":"B46","doi-asserted-by":"publisher","first-page":"e0004152","DOI":"10.1371\/journal.pntd.0004152","article-title":"T-Cell responses are associated with survival in acute melioidosis patients","volume":"9","author":"Jenjaroen","year":"2015","journal-title":"PLoS Negl. Trop. Dis"},{"key":"B47","doi-asserted-by":"publisher","first-page":"5189","DOI":"10.1073\/pnas.0706296105","article-title":"Antigen kinetics determines immune reactivity","volume":"105","author":"Johansen","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"B48","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1007\/s40475-017-0121-7","article-title":"Vaccines for the prevention of melioidosis and glanders","volume":"4","author":"Johnson","year":"2017","journal-title":"Curr. Trop. Med. Rep"},{"key":"B49","doi-asserted-by":"publisher","first-page":"783.e1","DOI":"10.1016\/j.cmi.2019.10.032","article-title":"Longitudinal profiling of plasma cytokines in melioidosis and their association with mortality: a prospective cohort study","volume":"26","author":"Kaewarpai","year":"2020","journal-title":"Clin. Microbiol. Infect."},{"key":"B50","doi-asserted-by":"publisher","first-page":"11","DOI":"10.3389\/fimmu.2013.00011","article-title":"Lipopolysaccharide induces IFN-\u03b3 production in human NK cells","volume":"4","author":"Kanevskiy","year":"2013","journal-title":"Front. Immunol"},{"key":"B51","doi-asserted-by":"publisher","first-page":"5657","DOI":"10.3390\/ijms20225657","article-title":"Defect of interferon \u03b3 leads to impaired wound healing through prolonged neutrophilic inflammatory response and enhanced MMP-2 activation","volume":"20","author":"Kanno","year":"2019","journal-title":"Int. J. Mol. Sci"},{"key":"B52","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1086\/341222","article-title":"Demonstration of a cell-mediated immune response in melioidosis","volume":"18","author":"Ketheesan","year":"2002","journal-title":"J. Infect. Dis"},{"key":"B53","doi-asserted-by":"publisher","first-page":"e00570","DOI":"10.1128\/mSphere.00570-18","article-title":"Burkholderia pseudomallei \u0394tonB \u0394hcp1 live attenuated vaccine strain elicits full protective immunity against aerosolized melioidosis infection","volume":"4","author":"Khakhum","year":"2019","journal-title":"mSphere"},{"key":"B54","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1038\/s41541-021-00333-4","article-title":"Antigen-specific antibody and polyfunctional T cells generated by respiratory immunization with protective Burkholderia \u0394tonB \u0394hcp1 live attenuated vaccines","volume":"6","author":"Khakhum","year":"2021","journal-title":"NPJ Vaccines"},{"key":"B55","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1080\/14760584.2020.1791089","article-title":"Combating the great mimicker: latest progress in the development of Burkholderia pseudomallei vaccines","volume":"19","author":"Khakhum","year":"2020","journal-title":"Exp. Rev. Vaccines"},{"key":"B56","doi-asserted-by":"publisher","first-page":"e00121","DOI":"10.1128\/mSphere.00121-17","article-title":"Gene expression profile of human cytokines in response to Burkholderia pseudomallei infection","volume":"2","author":"Krishnananthasivam","year":"2017","journal-title":"mSphere"},{"key":"B57","doi-asserted-by":"publisher","first-page":"ftw070","DOI":"10.1093\/femspd\/ftw070","article-title":"The art of persistence-the secrets to Burkholderia chronic infections","volume":"74","author":"Lewis","year":"2016","journal-title":"Pathog Dis."},{"key":"B58","doi-asserted-by":"publisher","first-page":"258","DOI":"10.3389\/fmicb.2014.00258","article-title":"The importance of the viable but non-culturable state in human bacterial pathogens","volume":"5","author":"Li","year":"2014","journal-title":"Front. Microbiol"},{"key":"B59","doi-asserted-by":"publisher","first-page":"8235","DOI":"10.1038\/srep08235","article-title":"Extended loop region of Hcp1 is critical for the assembly and function of type VI secretion system in Burkholderia pseudomallei","volume":"5","author":"Lim","year":"2015","journal-title":"Sci. Rep"},{"key":"B60","doi-asserted-by":"publisher","first-page":"e141480","DOI":"10.3201\/eid2106.141480","article-title":"Consensus on the development of vaccines against naturally acquired melioidosis","volume":"21","author":"Limmathurotsakul","year":"2015","journal-title":"Emerg. Infect. Dis"},{"key":"B61","doi-asserted-by":"publisher","first-page":"15008","DOI":"10.1038\/nmicrobiol.2015.8","article-title":"Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis","volume":"1","author":"Limmathurotsakul","year":"2016","journal-title":"Nat. Microbiol"},{"key":"B62","doi-asserted-by":"publisher","first-page":"2295","DOI":"10.1021\/acschembio.5b00502","article-title":"Burkholderia pseudomallei capsular polysaccharide recognition by a monoclonal antibody reveals key details toward a biodefense vaccine and diagnostics against melioidosis","volume":"10","author":"Marchetti","year":"2015","journal-title":"ACS Chem. Biol"},{"key":"B63","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/S0022-1759(98)00015-5","article-title":"The need for IgG2c specific antiserum when isotyping antibodies from C57BL\/6 and NOD mice","volume":"212","author":"Martin","year":"1998","journal-title":"J. Immunol. Methods"},{"key":"B64","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1165\/rcmb.2013-0142OC","article-title":"Proteinase-activated receptor-1, CCL2, and CCL7 regulate acute neutrophilic lung inflammation","volume":"50","author":"Mercer","year":"2014","journal-title":"Am. J. Respir. Cell Mol. Biol"},{"key":"B65","doi-asserted-by":"publisher","first-page":"958","DOI":"10.1038\/nri2448","article-title":"Exploring the full spectrum of macrophage activation","volume":"8","author":"Mosser","year":"2008","journal-title":"Nat. Rev. Immunol"},{"key":"B66","doi-asserted-by":"publisher","first-page":"1526","DOI":"10.1126\/science.1128393","article-title":"A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus","volume":"312","author":"Mougous","year":"2006","journal-title":"Science"},{"key":"B67","doi-asserted-by":"publisher","first-page":"723","DOI":"10.1038\/nri3073","article-title":"Protective and pathogenic functions of macrophage subsets","volume":"11","author":"Murray","year":"2011","journal-title":"Nat. Rev. Immunol"},{"key":"B68","doi-asserted-by":"publisher","first-page":"2251","DOI":"10.1084\/jem.20110919","article-title":"Regulation of neutrophils by interferon-\u03b3 limits lung inflammation during tuberculosis infection","volume":"208","author":"Nandi","year":"2011","journal-title":"J. Exp. Med"},{"key":"B69","volume-title":"Guide for the Care and Use of Laboratory Animals: Eighth Edition","year":"2011"},{"key":"B70","doi-asserted-by":"publisher","first-page":"1177","DOI":"10.1099\/jmm.0.45766-0","article-title":"Evaluation of lipopolysaccharide and capsular polysaccharide as subunit vaccines against experimental melioidosis","volume":"53","author":"Nelson","year":"2004","journal-title":"J. Med. Microbiol"},{"key":"B71","doi-asserted-by":"publisher","first-page":"8381","DOI":"10.1016\/j.vaccine.2011.08.058","article-title":"A naturally derived outer-membrane vesicle vaccine protects against lethal pulmonary Burkholderia pseudomallei infection","volume":"29","author":"Nieves","year":"2011","journal-title":"Vaccine"},{"key":"B72","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1128\/CVI.00119-14","article-title":"A Burkholderia pseudomallei outer membrane vesicle vaccine provides protection against lethal sepsis","volume":"21","author":"Nieves","year":"2014","journal-title":"Clin. Vaccine Immunol"},{"key":"B73","doi-asserted-by":"publisher","first-page":"4010","DOI":"10.1128\/IAI.05044-11","article-title":"The Burkholderia pseudomallei \u0394asd mutant exhibits attenuated intracellular infectivity and imparts protection against acute inhalation melioidosis in mice","volume":"79","author":"Norris","year":"2011","journal-title":"Infect. Immun"},{"key":"B74","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1128\/iai.56.2.419-423.1988","article-title":"Characterization of aromatic- and purine-dependent Salmonella typhimurium: attention, persistence, and ability to induce protective immunity in BALB\/c mice","volume":"56","author":"O'Callaghan","year":"1988","journal-title":"Infect. Immun"},{"key":"B75","doi-asserted-by":"publisher","first-page":"3876","DOI":"10.1016\/j.vaccine.2012.04.002","article-title":"Alkyl hydroperoxide reductase: a candidate Helicobacter pylori vaccine","volume":"30","author":"O'Riordan","year":"2012","journal-title":"Vaccine"},{"key":"B76","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1007\/s12199-015-0493-z","article-title":"Comparison between whole-body inhalation and nose-only inhalation on the deposition and health effects of nanoparticles","volume":"21","author":"Oyabu","year":"2016","journal-title":"Environ. Health Prev. Med"},{"key":"B77","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1016\/j.diagmicrobio.2006.04.018","article-title":"Polysaccharide microarray technology for the detection of Burkholderia pseudomallei and Burkholderia mallei antibodies","volume":"56","author":"Parthasarathy","year":"2006","journal-title":"Diagn. Microbiol. Infect. Dis"},{"key":"B78","doi-asserted-by":"publisher","first-page":"e1488","DOI":"10.1371\/journal.pntd.0001488","article-title":"Melioidosis vaccines: a systematic review and appraisal of the potential to exploit biodefense vaccines for public health purposes","volume":"6","author":"Peacock","year":"2012","journal-title":"PLoS Negl. Trop. Dis"},{"key":"B79","doi-asserted-by":"publisher","first-page":"878","DOI":"10.1128\/IAI.01529-06","article-title":"Vaccination with live Yersinia pestis primes CD4 and CD8 T cells that synergistically protect against lethal pulmonary Y. pestis infection","volume":"75","author":"Philipovskiy","year":"2007","journal-title":"Infect. Immun"},{"key":"B80","doi-asserted-by":"publisher","first-page":"e00346","DOI":"10.1128\/JCM.00346-18","article-title":"A rapid immunochromatography test based on Hcp1 is a potential point-of-care test for serological diagnosis of melioidosis","volume":"56","author":"Phokrai","year":"2018","journal-title":"J. Clin. Microbiol"},{"key":"B81","doi-asserted-by":"publisher","first-page":"1286","DOI":"10.1016\/j.ajpath.2012.12.007","article-title":"IL-22 is essential for lung epithelial repair following influenza infection","volume":"182","author":"Pociask","year":"2013","journal-title":"Am. J. Pathol"},{"key":"B82","doi-asserted-by":"publisher","first-page":"5983","DOI":"10.1016\/j.vaccine.2014.08.035","article-title":"Comparison of the protective effects of killed Burkholderia pseudomallei and CpG oligodeoxynucleotide against live challenge","volume":"32","author":"Puangpetch","year":"2014","journal-title":"Vaccine"},{"key":"B83","doi-asserted-by":"publisher","first-page":"15508","DOI":"10.1073\/pnas.0706532104","article-title":"Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin","volume":"104","author":"Pukatzki","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"B84","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1016\/j.trstmh.2011.06.004","article-title":"Survival of Burkholderia pseudomallei in distilled water for 16 years","volume":"105","author":"Pumpuang","year":"2011","journal-title":"Trans. R. Soc. Trop. Med. Hyg"},{"key":"B85","doi-asserted-by":"publisher","first-page":"e0005499","DOI":"10.1371\/journal.pntd.0005499","article-title":"Comparison of O-polysaccharide and hemolysin co-regulated protein as target antigens for serodiagnosis of melioidosis","volume":"11","author":"Pumpuang","year":"2017","journal-title":"PLoS Negl. Trop. Dis"},{"key":"B86","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1016\/j.cytogfr.2014.07.010","article-title":"IL-4 and IL-13 receptors: Roles in immunity and powerful vaccine adjuvants","volume":"25","author":"Ranasinghe","year":"2014","journal-title":"Cytokine Growth Fact. Rev"},{"key":"B87","doi-asserted-by":"publisher","first-page":"4814","DOI":"10.4049\/jimmunol.1402862","article-title":"T cell immunity to the alkyl hydroperoxide reductase of Burkholderia pseudomallei: a correlate of disease outcome in acute melioidosis","volume":"194","author":"Reynolds","year":"2015","journal-title":"J. Immunol"},{"key":"B88","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/B978-0-12-811924-2.00019-5","article-title":"Current and new approaches for mucosal vaccine delivery","author":"Rhee","year":"2020","journal-title":"Mucosal. Vaccines"},{"key":"B89","doi-asserted-by":"publisher","first-page":"3593","DOI":"10.1128\/IAI.67.7.3593-3600.1999","article-title":"Obligatory role of \u03b3 interferon for host survival in a murine model of infection with Burkholderia pseudomallei","volume":"67","author":"Santanirand","year":"1999","journal-title":"Infect. Immun"},{"key":"B90","doi-asserted-by":"publisher","first-page":"4447","DOI":"10.1016\/j.vaccine.2009.05.040","article-title":"Protective efficacy of heat-inactivated B. thailandensis, B. mallei or B. pseudomallei against experimental melioidosis and glanders","volume":"27","author":"Sarkar-Tyson","year":"2009","journal-title":"Vaccine"},{"key":"B91","doi-asserted-by":"publisher","first-page":"33123","DOI":"10.1038\/srep33123","article-title":"Chemokine CXCL1 mediated neutrophil recruitment: role of glycosaminoglycan interactions","volume":"6","author":"Sawant","year":"2016","journal-title":"Sci. Rep"},{"key":"B92","doi-asserted-by":"publisher","first-page":"e0022222","DOI":"10.1128\/iai.00222-22","article-title":"Development of melioidosis subunit vaccines using and enzymatically inactive Burkholderia pseudomallei AhpC","volume":"11","author":"Schmidt","year":"2022","journal-title":"Infect. Immunity"},{"key":"B93","doi-asserted-by":"publisher","first-page":"3206","DOI":"10.1128\/IAI.01847-14","article-title":"Burkholderia pseudomallei capsular polysaccharide conjugates provide protection against acute melioidosis","volume":"82","author":"Scott","year":"","journal-title":"Infect. Immun"},{"key":"B94","doi-asserted-by":"publisher","first-page":"1435","DOI":"10.1021\/acs.bioconjchem.5b00525","article-title":"Protection against experimental melioidosis with a synthetic manno-heptopyranose hexasaccharide glycoconjugate","volume":"27","author":"Scott","year":"2016","journal-title":"Bioconjug. Chem"},{"key":"B95","doi-asserted-by":"publisher","first-page":"392170","DOI":"10.1155\/2014\/392170","article-title":"Protection against experimental melioidosis following immunisation with a lipopolysaccharide-protein conjugate","volume":"2014","author":"Scott","year":"","journal-title":"J. Immunol. Res"},{"key":"B96","doi-asserted-by":"publisher","first-page":"698303","DOI":"10.3389\/fimmu.2021.698303","article-title":"Melioidosis patient survival correlates with strong IFN-\u03b3 secreting T cell responses against Hcp1 and TssM","volume":"12","author":"Sengyee","year":"2021","journal-title":"Front. Immunol"},{"key":"B97","doi-asserted-by":"publisher","first-page":"10","DOI":"10.3389\/fcimb.2013.00010","article-title":"Development of Burkholderia mallei and pseudomallei vaccines","volume":"3","author":"Silva","year":"2013","journal-title":"Front. Cell Infect. Microbiol"},{"key":"B98","doi-asserted-by":"publisher","first-page":"4626","DOI":"10.1128\/IAI.00915-13","article-title":"Correlates of immune protection following cutaneous immunization with an attenuated Burkholderia pseudomallei vaccine","volume":"81","author":"Silva","year":"2013","journal-title":"Infect. Immun"},{"key":"B99","doi-asserted-by":"publisher","first-page":"3542","DOI":"10.1016\/j.vaccine.2015.12.077","article-title":"Differential regulation of polysaccharide-specific antibody responses to isolated polysaccharides, conjugate vaccines, and intact gram-positive versus gram-negative extracellular bacteria","volume":"34","author":"Snapper","year":"2016","journal-title":"Vaccine"},{"key":"B100","doi-asserted-by":"publisher","first-page":"968","DOI":"10.1186\/s12879-019-4618-7","article-title":"Diagnostic and prognostic value of interleukin-6, pentraxin 3, and procalcitonin levels among sepsis and septic shock patients: a prospective controlled study according to the Sepsis-3 definitions","volume":"19","author":"Song","year":"2019","journal-title":"BMC Infect. Dis"},{"key":"B101","doi-asserted-by":"publisher","first-page":"270","DOI":"10.2119\/molmed.2011.00201","article-title":"Natural killer (NK) cells in antibacterial innate immunity: angels or devils?","volume":"18","author":"Souza-Fonseca-Guimaraes","year":"2012","journal-title":"Mol. Med"},{"key":"B102","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1086\/317537","article-title":"Type 1\/Type 2 immunity in infectious diseases","volume":"32","author":"Spellberg","year":"2001","journal-title":"Clin. Infect. Dis"},{"key":"B103","first-page":"123","article-title":"Construction and characterization of an unmarked aroC deletion mutant of Burkholderia pseudomallei strain A2","volume":"40","author":"Srilunchang","year":"2009","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"B104","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1056\/NEJM200107263450404","article-title":"Glanders in a military research microbiologist","volume":"34","author":"Srinivasan","year":"2001","journal-title":"N. Engl. J. Med"},{"key":"B105","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1080\/15298668891379503","article-title":"Nose-only versus whole-body aerosol exposure for induction of upper respiratory infections of laboratory mice","volume":"49","author":"Stephenson","year":"1988","journal-title":"Am. Ind. Hyg. Assoc. J"},{"key":"B106","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1046\/j.1365-2958.2002.03190.x","article-title":"An Inv\/Mxi-Spa-like type III protein secretion system in Burkholderia pseudomallei modulates intracellular behaviour of the pathogen","volume":"46","author":"Stevens","year":"2002","journal-title":"Mol. Microbiol"},{"key":"B107","doi-asserted-by":"publisher","first-page":"e0122721","DOI":"10.1128\/mBio.01227-21","article-title":"Multicomponent gold-linked glycoconjugate vaccine elicits antigen-specific humoral and mixed TH1-TH17 immunity, correlated with increased protection against Burkholderia pseudomallei","volume":"12","author":"Tapia","year":"2021","journal-title":"mBio"},{"key":"B108","doi-asserted-by":"publisher","first-page":"153","DOI":"10.3390\/vetsci7040153","article-title":"Humoral immune responses to Burkholderia pseudomallei antigens in captive and wild macaques in the western part of Java, Indonesia","volume":"7","author":"Testamenti","year":"2020","journal-title":"Vet. Sci."},{"key":"B109","doi-asserted-by":"publisher","first-page":"e407","DOI":"10.1371\/journal.pntd.0000407","article-title":"Phenotypic and functional characterization of human memory T cell responses to Burkholderia pseudomallei","volume":"3","author":"Tippayawat","year":"2009","journal-title":"PLoS Negl. Trop. Dis"},{"key":"B110","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1128\/MMBR.05015-11","article-title":"Into the eye of the cytokine storm","volume":"76","author":"Tisoncik","year":"2012","journal-title":"Microbiol. Mol. Biol. Rev"},{"key":"B111","doi-asserted-by":"publisher","first-page":"5981","DOI":"10.1016\/j.vaccine.2017.03.022","article-title":"Burkholderia pseudomallei and Burkholderia mallei vaccines: are we close to clinical trials?","volume":"35","author":"Titball","year":"2017","journal-title":"Vaccine"},{"key":"B112","doi-asserted-by":"publisher","first-page":"767359","DOI":"10.3389\/fimmu.2021.767359","article-title":"BpOmpW antigen stimulates the necessary protective T-cell responses against melioidosis","volume":"12","author":"Tomas-Cortazar","year":"2021","journal-title":"Front. Immunol"},{"key":"B113","doi-asserted-by":"publisher","first-page":"e0009125","DOI":"10.1371\/journal.pntd.0009125","article-title":"Comparative virulence of three different strains of Burkholderia pseudomallei in an aerosol non-human primate model","volume":"15","author":"Trevino","year":"2021","journal-title":"PLoS Negl. Trop. Dis"},{"key":"B114","doi-asserted-by":"publisher","first-page":"e0208277","DOI":"10.1371\/journal.pone.0208277","article-title":"Disease progression in mice exposed to low-doses of aerosolized clinical isolates of Burkholderia pseudomallei","volume":"13","author":"Trevino","year":"2018","journal-title":"PLoS ONE"},{"key":"B115","doi-asserted-by":"publisher","first-page":"ftw111","DOI":"10.1093\/femspd\/ftw111","article-title":"Cytokines IL-17 and IL-22 in the host response to infection","volume":"74","author":"Valeri","year":"2016","journal-title":"Pathog Dis"},{"key":"B116","doi-asserted-by":"publisher","first-page":"120","DOI":"10.3389\/fcimb.2012.00120","article-title":"An objective approach for Burkholderia pseudomallei strain selection as challenge material for medical countermeasures efficacy testing","volume":"2","author":"Van Zandt","year":"2012","journal-title":"Front. Cell Infect. Microbiol"},{"key":"B117","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1128\/CDLI.9.3.530-543.2002","article-title":"Endotoxin-induced \u03b3 interferon production: contributing cell types and key regulatory factors","volume":"9","author":"Varma","year":"2002","journal-title":"Clin. Diagn. Lab Immunol"},{"key":"B118","doi-asserted-by":"publisher","first-page":"2671","DOI":"10.3390\/cells9122671","article-title":"Current advances in Burkholderia vaccines development","volume":"9","author":"Wang","year":"2020","journal-title":"Cells"},{"key":"B119","doi-asserted-by":"publisher","first-page":"5252","DOI":"10.1128\/IAI.00824-09","article-title":"Role for the Burkholderia pseudomallei capsular polysaccharide encoded by the wcb operon in acute disseminated melioidosis","volume":"77","author":"Warawa","year":"2009","journal-title":"Infect. Immun"},{"key":"B120","doi-asserted-by":"publisher","first-page":"e0124667","DOI":"10.1371\/journal.pone.0124667","article-title":"Characterization of Burkholderia pseudomallei strains using a murine intraperitoneal infection model and in vitro macrophage assays","volume":"10","author":"Welkos","year":"2015","journal-title":"PLoS ONE"},{"key":"B121","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1186\/1471-2172-9-55","article-title":"Host immunity in the protective response to vaccination with heat-killed Burkholderia mallei","volume":"9","author":"Whitlock","year":"2008","journal-title":"BMC Immunol"},{"key":"B122","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.1056\/NEJMra1204699","article-title":"Melioidosis","volume":"367","author":"Wiersinga","year":"2012","journal-title":"N. Engl. J. Med"},{"key":"B123","doi-asserted-by":"publisher","first-page":"4548","DOI":"10.1016\/j.vaccine.2013.07.062","article-title":"IL-4 and IL-13 mediated down-regulation of CD8 expression levels can dampen anti-viral CD8(+) T cell avidity following HIV-1 recombinant pox viral vaccination","volume":"31","author":"Wijesundara","year":"2013","journal-title":"Vaccine"},{"key":"B124","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1093\/cid\/ciaa126","article-title":"A 2-biomarker model augments clinical prediction of mortality in melioidosis","volume":"72","author":"Wright","year":"2021","journal-title":"Clin. Infect. Dis"},{"key":"B125","doi-asserted-by":"publisher","first-page":"205","DOI":"10.3109\/08958379009145255","article-title":"Comparative evaluation of nose-only versus whole-body inhalation exposures for rats\u2014aerosol characteristics and lung deposition, inhalation Toxicology","author":"Yeh","year":"1990"},{"key":"B126","doi-asserted-by":"publisher","first-page":"20161120","DOI":"10.1101\/082057","article-title":"Immunological patterns from four melioidosis cases: constant and variable protein antigens","volume":"2016","author":"Yi","year":"2016","journal-title":"bioRxiv"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.965518\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T01:35:04Z","timestamp":1676424904000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.965518\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,17]]},"references-count":126,"alternative-id":["10.3389\/fmicb.2022.965518"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2022.965518","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,17]]},"article-number":"965518"}}