{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T08:30:00Z","timestamp":1783758600210,"version":"3.55.0"},"reference-count":67,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,10,5]],"date-time":"2022-10-05T00:00:00Z","timestamp":1664928000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006506","name":"Brazilian Ministry of Health","doi-asserted-by":"publisher","award":["442653\/2019-0"],"award-info":[{"award-number":["442653\/2019-0"]}],"id":[{"id":"10.13039\/501100006506","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["INV-003970"],"award-info":[{"award-number":["INV-003970"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.499061\/2020-00"],"award-info":[{"award-number":["88887.499061\/2020-00"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Antibiotic treatment has been used to enhance anopheline susceptibility to <jats:italic>Plasmodium<\/jats:italic> infection, because bacterial microbiota play a fundamental role in modulating the vector competence of mosquitoes that transmit <jats:italic>Plasmodium<\/jats:italic> parasites. However, few studies have examined the impact of antibiotic treatments on <jats:italic>Plasmodium vivax<\/jats:italic> sporogonic development in neotropical anopheline mosquitoes. Herein, we assessed the impact of antibiotic treatment on <jats:italic>P. vivax<\/jats:italic> development and survival in <jats:italic>Anopheles darlingi<\/jats:italic>, the main vector of malaria in the Amazon region. Female mosquitoes were treated continuously with antibiotics to impact the gut bacterial load and then tested for prevalence, infection intensity, and survival in comparison with untreated mosquitoes. Antibiotic-fed mosquitoes had not dramatic impact on <jats:italic>P. vivax<\/jats:italic> development previously observed in <jats:italic>P. falciparum<\/jats:italic>. However, antibiotic treatment increases mosquito survival, which is known to increase vectorial capacity. These findings raise questions about the effect of antibiotics on <jats:italic>P. vivax<\/jats:italic> development and survival in <jats:italic>An. darlingi<\/jats:italic>.<\/jats:p>","DOI":"10.3389\/fmicb.2022.971083","type":"journal-article","created":{"date-parts":[[2022,10,6]],"date-time":"2022-10-06T13:39:04Z","timestamp":1665063544000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Assessment of antibiotic treatment on Anopheles darlingi survival and susceptibility to Plasmodium vivax"],"prefix":"10.3389","volume":"13","author":[{"given":"Najara Akira Costa dos","family":"Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Neves","family":"Magi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice Oliveira","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra da Silva","family":"Bastos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soraya dos Santos","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jansen Fernandes","family":"Medeiros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maisa da Silva","family":"Araujo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,10,5]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"e20190159","DOI":"10.1590\/0037-8682-0159-2019","article-title":"Brazil\u2019s first free-mating laboratory colony of Nyssorhynchus darlingi","volume":"52","author":"Araujo","year":"2019","journal-title":"Rev. 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J."},{"key":"ref35","volume-title":"Methods in Malaria Research","author":"Moll","year":"2013"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"612","DOI":"10.4269\/ajtmh.13-0708","article-title":"Infection of laboratory-colonized Anopheles darlingi mosquitoes by Plasmodium vivax","volume":"90","author":"Moreno","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1021\/acsinfecdis.7b00195","article-title":"Continuous supply of Plasmodium vivax sporozoites from colonized Anopheles darlingi in the Peruvian Amazon","volume":"4","author":"Moreno","year":"2018","journal-title":"ACS Infect. 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Parasitol."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"e1004398","DOI":"10.1371\/journal.ppat.1004398","article-title":"Chromobacterium Csp_P reduces malaria and dengue infection in vector mosquitoes and has entomopathogenic and in vitro anti-pathogen activities","volume":"10","author":"Ramirez","year":"2014","journal-title":"PLoS Pathog."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1186\/s12936-015-0718-z","article-title":"Nowhere to hide: interrogating different metabolic parameters of Plasmodium falciparum gametocytes in a transmission blocking drug discovery pipeline towards malaria elimination","volume":"14","author":"Reader","year":"2015","journal-title":"Malar. J."},{"key":"ref42","first-page":"7","article-title":"Epidemiology","volume-title":"Arthropod-Borne Diseases","author":"Reisen","year":"2017"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1111\/j.1462-2920.2010.02395.x","article-title":"The yeast Wickerhamomyces anomalus (Pichia anomala) inhabits the midgut and reproductive system of the Asian malaria vector Anopheles stephensi","volume":"13","author":"Ricci","year":"2011","journal-title":"Environ. Microbiol."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"161","DOI":"10.3390\/tropicalmed5040161","article-title":"Vector-focused approaches to curb malaria transmission in the Brazilian Amazon: an over review of current and future challenges and strategies","volume":"5","author":"Rocha","year":"2020","journal-title":"Trop. Med. Infect. Dis."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"e1006391","DOI":"10.1371\/journal.ppat.1006391","article-title":"Microbiota-induced peritrophic matrix regulates midgut homeostasis and prevents systemic infection of malaria vector mosquitoes","volume":"13","author":"Rodgers","year":"2017","journal-title":"PLoS Pathog."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"e0008072","DOI":"10.1371\/journal.pntd.0008072","article-title":"Human Plasmodium vivax diversity, population structure and evolutionary origin","volume":"14","author":"Rougeron","year":"2020","journal-title":"PLoS Negl. Trop. Dis."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1186\/s12936-022-04204-8","article-title":"Evaluation of sustainable susceptibility to Plasmodium vivax infection among colonized Anopheles darlingi and Anopheles deaneorum","volume":"21","author":"Santos","year":"2022","journal-title":"Malar. J."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/S0176-6724(87)80029-9","article-title":"Concomitant infections of Anopheles stephensi with Plasmodium berghei and Serratia marcescens: additive detrimental effects","volume":"266","author":"Seitz","year":"1987","journal-title":"Zentralb Bakteriol Mikrobiol Hyg A"},{"key":"ref49","doi-asserted-by":"publisher","first-page":"269","DOI":"10.3389\/fmicb.2020.00269","article-title":"Critical steps of Plasmodium falciparum ookinete maturation","volume":"11","author":"Siciliano","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1017\/s0031182000057334","article-title":"The development of Plasmodium ookinetes in vitro: an ultrastructural study including a description of meiotic division","volume":"91","author":"Sinden","year":"1985","journal-title":"Parasitology"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.actatropica.2013.06.008","article-title":"Gut microbes influence fitness and malaria transmission potential of Asian malaria vector Anopheles stephensi","volume":"128","author":"Sharma","year":"2013","journal-title":"Acta Trop."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"609","DOI":"10.3389\/fimmu.2020.00609","article-title":"Altered gut microbiota and immunity defines Plasmodium vivax survival in Anopheles stephensi","volume":"11","author":"Sharma","year":"2020","journal-title":"Front. Immunol."},{"key":"ref53","doi-asserted-by":"publisher","first-page":"e1006899","DOI":"10.1371\/journal.ppat.1006899","article-title":"PGRP-LD mediates A. stephensi vector competency by regulating homeostasis of microbiota-induced peritrophic matrix synthesis","volume":"14","author":"Song","year":"2018","journal-title":"PLoS Pathog."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1098\/rstb.1980.0045","article-title":"Biochemical and genetical approaches to the mechanism of action of penicillin","volume":"289","author":"Spratt","year":"1980","journal-title":"Philos. Trans. R. Soc. Lond."},{"key":"ref55","doi-asserted-by":"publisher","first-page":"703","DOI":"10.4269\/ajtmh.1940.s1-20.703","article-title":"The influence of temperature on Plasmodium vivax","volume":"20","author":"Stratman-Thomas","year":"1940","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1590\/S0036-46652000000200005","article-title":"Malaria vectors in the Brazilian Amazon: Anopheles of the subgenus Nyssorhynchus","volume":"42","author":"Tadei","year":"2000","journal-title":"Rev. Inst. Med. Trop. S. Paulo."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1093\/jmedent\/37.2.246","article-title":"Bactericidal effects of sugar-fed antibiotics on resident midgut bacteria of newly emerged anopheline mosquitoes (Diptera: Culicidae)","volume":"37","author":"Tour\u00e9","year":"2000","journal-title":"J. Med. 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