{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T11:19:56Z","timestamp":1788520796045,"version":"build-2803163510"},"reference-count":68,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T00:00:00Z","timestamp":1664323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Antibiotic resistance genes (ARGs) in the environment pose a threat to human and animal health. Dairy cows are important livestock in China; however, a comprehensive understanding of antibiotic resistance in their production environment has not been well clarified. In this study, we used metagenomic methods to analyze the resistomes, microbiomes, and potential ARG bacterial hosts in typical dairy farm environments (including feces, wastewater, and soil). The ARGs resistant to tetracyclines, MLS, \u03b2-lactams, aminoglycoside, and multidrug was dominant in the dairy farm ecosystem. The abundance and diversity of total ARGs in dairy feces and wastewater were significantly higher than in soil (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&amp;lt;\u20090.05). The same environmental samples from different dairy have similar resistomes and microbiomes. A high detection rate of\n                    <jats:italic>tet<\/jats:italic>\n                    (X) in wastewater and feces (100% and 71.4%, respectively), high abundance (range from 5.74 to 68.99\u2009copies\/Gb), and the finding of\n                    <jats:italic>tet<\/jats:italic>\n                    (X5) challenged the clinical application of the last antibiotics resort of tigecycline. Network analysis identified\n                    <jats:italic>Bacteroides<\/jats:italic>\n                    as the dominant genus in feces and wastewater, which harbored the greatest abundance of their respective total ARG coverage and shared ARGs. These results improved our understanding of ARG profiles and their bacterial hosts in dairy farm environments and provided a basis for further surveillance.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2022.990272","type":"journal-article","created":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T10:12:02Z","timestamp":1664359922000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":29,"title":["Metagenomic insights into the antibiotic resistomes of typical Chinese dairy farm environments"],"prefix":"10.3389","volume":"13","author":[{"given":"Jijun","family":"Kang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojie","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenguang","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiubo","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,28]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"590","DOI":"10.1089\/mdr.2017.0147","article-title":"Vancomycin-resistant enterococci: a review of antimicrobial resistance mechanisms and perspectives of human and animal health","volume":"24","author":"Ahmed","year":"2018","journal-title":"Microb. Drug Resist."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.ijid.2016.11.415","article-title":"How should we respond to the emergence of plasmid-mediated colistin resistance in humans and animals?","volume":"54","author":"Al-Tawfiq","year":"2017","journal-title":"Int. J. Infect. Dis."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1186\/s40168-018-0401-z","article-title":"DeepARG: a deep learning approach for predicting antibiotic resistance genes from metagenomic data","volume":"6","author":"Arango-Argoty","year":"2018","journal-title":"Microbiome"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"e0133764","DOI":"10.1371\/journal.pone.0133764","article-title":"Metagenomic analysis of antibiotic resistance genes in dairy cow feces following therapeutic administration of third generation cephalosporin","volume":"10","author":"Chambers","year":"2015","journal-title":"PLoS One"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1089\/fpd.2018.2560","article-title":"In vitro antimicrobial resistance of causative agents to clinical mastitis in Danish dairy cows","volume":"16","author":"Chehabi","year":"2019","journal-title":"Foodborne Pathog. 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Pollut."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.jhazmat.2015.10.037","article-title":"Behavior of antibiotics and antibiotic resistance genes in eco-agricultural system: a case study","volume":"304","author":"Cheng","year":"2016","journal-title":"J. Hazard. Mater."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1007\/s00248-016-0866-x","article-title":"Functional Metagenomics as a tool for identification of new antibiotic resistance genes from natural environments","volume":"73","author":"Dos Santos","year":"2017","journal-title":"Microb. Ecol."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/j.ecoenv.2019.05.005","article-title":"Factors that affect the occurrence and distribution of antibiotic resistance genes in soils from livestock and poultry farms","volume":"180","author":"Duan","year":"2019","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"1477","DOI":"10.1016\/j.scitotenv.2018.04.020","article-title":"Tracking antibiotic resistance genes in soil irrigated with dairy wastewater","volume":"635","author":"Dungan","year":"2018","journal-title":"Sci. Total Environ."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"2893","DOI":"10.1093\/jac\/dky240","article-title":"Antibiotic use in people and pigs: a one health survey of rural residents' knowledge, attitudes and practices in Shandong province China","volume":"73","author":"Dyar","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1111\/1462-2920.14009","article-title":"Antibiotic resistome in a large-scale healthy human gut microbiota deciphered by metagenomic and network analyses","volume":"20","author":"Feng","year":"2018","journal-title":"Environ. Microbiol."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"273","DOI":"10.3390\/antibiotics9050273","article-title":"Antibiotic consumption on dairy and beef cattle farms of Central Italy based on paper registers","volume":"9","author":"Ferroni","year":"2020","journal-title":"Antibiotics"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"1163","DOI":"10.1101\/gr.155465.113","article-title":"Country-specific antibiotic use practices impact the human gut resistome","volume":"23","author":"Forslund","year":"2013","journal-title":"Genome Res."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"124921","DOI":"10.1016\/j.jhazmat.2020.124921","article-title":"Abundance of tigecycline resistance genes and association with antibiotic residues in Chinese livestock farms","volume":"409","author":"Fu","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"5010","DOI":"10.1111\/1462-2920.13949","article-title":"Metagenomic assessment of the interplay between the environment and the genetic diversification of Acinetobacter","volume":"19","author":"Garcia-Garcera","year":"2017","journal-title":"Environ. Microbiol."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"146","DOI":"10.3389\/fmicb.2019.00146","article-title":"Comparative Metagenomics and network analyses provide novel insights into the scope and distribution of beta-lactamase homologs in the environment","volume":"10","author":"Gatica","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"e2019GH000213","DOI":"10.1029\/2019GH000213","article-title":"A review of potential public health impacts associated with the global dairy sector","volume":"4","author":"Grout","year":"2020","journal-title":"Geohealth"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"1450","DOI":"10.1038\/s41564-019-0445-2","article-title":"Emergence of plasmid-mediated high-level tigecycline resistance genes in animals and humans","volume":"4","author":"He","year":"2019","journal-title":"Nat. Microbiol."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/j.envint.2017.07.003","article-title":"Environmental and human health challenges of industrial livestock and poultry farming in China and their mitigation","volume":"107","author":"Hu","year":"2017","journal-title":"Environ. 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Technol."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1038\/nprot.2015.046","article-title":"Optimized DNA extraction and metagenomic sequencing of airborne microbial communities","volume":"10","author":"Jiang","year":"2015","journal-title":"Nat. Protoc."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1016\/j.anaerobe.2017.06.004","article-title":"Erm gene distribution among Norwegian Bacteroides isolates and evaluation of phenotypic tests to detect inducible clindamycin resistance in Bacteroides species","volume":"47","author":"Johnsen","year":"2017","journal-title":"Anaerobe"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"2360","DOI":"10.1016\/j.csbj.2022.05.010","article-title":"Towards discovery of inhibitors of the undecaprenyl-pyrophosphate phosphatase BacA by virtual high-throughput screening","volume":"20","author":"Jukic","year":"2022","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.anaerobe.2017.12.011","article-title":"An in vitro study to assess the impact of tetracycline on the human intestinal microbiome","volume":"49","author":"Jung","year":"2017","journal-title":"Anaerobe"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"1652","DOI":"10.1093\/nar\/gkp1142","article-title":"Insights into the structure, function and evolution of the radical-SAM 23S rRNA methyltransferase Cfr that confers antibiotic resistance in bacteria","volume":"38","author":"Kaminska","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1016\/j.jhin.2015.11.010","article-title":"Tigecycline as last resort in severe refractory Clostridium difficile infection: a case report","volume":"92","author":"Knafl","year":"2016","journal-title":"J. Hosp. Infect."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/j.tvjl.2015.08.013","article-title":"Recycling manure as cow bedding: potential benefits and risks for UK dairy farms","volume":"206","author":"Leach","year":"2015","journal-title":"Vet. J."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/1040841X.2020.1715339","article-title":"Prevalence of extended-spectrum beta-lactamases in the local farm environment and livestock: challenges to mitigate antimicrobial resistance","volume":"46","author":"Lee","year":"2020","journal-title":"Crit. Rev. Microbiol."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/gigascience\/giaa043","article-title":"Antibiotic resistomes discovered in the gut microbiomes of Korean swine and cattle","volume":"9","author":"Lim","year":"2020","journal-title":"Gigascience"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"113058","DOI":"10.1016\/j.envpol.2019.113058","article-title":"Dairy farm soil presents distinct microbiota and varied prevalence of antibiotic resistance across housing areas","volume":"254","author":"Liu","year":"2019","journal-title":"Environ. Pollut."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"2500","DOI":"10.1128\/AAC.00131-06","article-title":"The Cfr rRNA methyltransferase confers resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin a antibiotics","volume":"50","author":"Long","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"33","DOI":"10.3390\/pathogens10010033","article-title":"Rapid increase in the IS26-mediated cfr gene in E. coli isolates with IncP and IncX4 plasmids and co-existing cfr and mcr-1 genes in a swine farm","volume":"10","author":"Ma","year":"2021","journal-title":"Pathogens"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1021\/acs.est.5b03522","article-title":"Metagenomic assembly reveals hosts of antibiotic resistance genes and the shared Resistome in pig, chicken, and human feces","volume":"50","author":"Ma","year":"2016","journal-title":"Environ. Sci. Technol."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/j.ijantimicag.2009.02.008","article-title":"Study on the dissemination of the bcrABDR cluster in enterococcus spp. reveals that the BcrAB transporter is sufficient to confer high-level bacitracin resistance","volume":"34","author":"Matos","year":"2009","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"24645","DOI":"10.1038\/srep24645","article-title":"Characterization of the resistome in manure, soil and wastewater from dairy and beef production systems","volume":"6","author":"Noyes","year":"2016","journal-title":"Sci. Rep."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"2797","DOI":"10.1093\/jac\/dkaa284","article-title":"Preliminary view of the global distribution and spread of the tet(X) family of tigecycline resistance genes","volume":"75","author":"Pan","year":"2020","journal-title":"J. Antimicrob. Chemother."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1089\/fpd.2015.2092","article-title":"Metagenomic evidence of the prevalence and distribution patterns of antimicrobial resistance genes in dairy agroecosystems","volume":"13","author":"Pitta","year":"2016","journal-title":"Foodborne Pathog. Dis."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1186\/s40793-020-00368-5","article-title":"The distribution of microbiomes and resistomes across farm environments in conventional and organic dairy herds in Pennsylvania","volume":"15","author":"Pitta","year":"2020","journal-title":"Environ. Microbiome"},{"key":"ref44","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1016\/j.ijantimicag.2010.10.031","article-title":"Activity of tigecycline alone and in combination with colistin and meropenem against Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae strains by time-kill assay","volume":"37","author":"Pournaras","year":"2011","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref45","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.jhazmat.2016.05.002","article-title":"Variable effects of oxytetracycline on antibiotic resistance gene abundance and the bacterial community during aerobic composting of cow manure","volume":"315","author":"Qian","year":"2016","journal-title":"J. Hazard. Mater."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1016\/j.envint.2017.10.016","article-title":"Review of antibiotic resistance in China and its environment","volume":"110","author":"Qiao","year":"2018","journal-title":"Environ. Int."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"1980","DOI":"10.3389\/fmicb.2019.01980","article-title":"Characterization of the microbial resistome in conventional and \"raised without antibiotics\" beef and dairy production systems","volume":"10","author":"Rovira","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1007\/s11270-016-2979-8","article-title":"Antibiotic resistance in airborne bacteria near conventional and organic beef cattle farms in California, USA","volume":"227","author":"Sancheza","year":"2016","journal-title":"Water Air Soil Pollut."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"634","DOI":"10.1111\/jam.14994","article-title":"Characterization and virulence factors distribution of Bla(CTX-M) and mcr-1carrying Escherichia coli isolates from bovine mastitis","volume":"131","author":"Shafiq","year":"2021","journal-title":"J. Appl. Microbiol."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"1234","DOI":"10.13345\/j.cjb.180177","article-title":"Antibiotic resistance in environment of animal farms","volume":"34","author":"Shi","year":"2018","journal-title":"Sheng Wu Gong Cheng Xue Bao"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1016\/j.envpol.2016.03.038","article-title":"Distribution of antibiotic resistance genes (ARGs) in anaerobic digestion and land application of swine wastewater","volume":"213","author":"Sui","year":"2016","journal-title":"Environ. Pollut."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"9048","DOI":"10.1007\/s11356-015-5891-7","article-title":"Seasonal variation and removal efficiency of antibiotic resistance genes during wastewater treatment of swine farms","volume":"24","author":"Sui","year":"2017","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref53","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.scitotenv.2016.12.138","article-title":"Impact of dairy manure pre-application treatment on manure composition, soil dynamics of antibiotic resistance genes, and abundance of antibiotic-resistance genes on vegetables at harvest","author":"Tien","year":"2017","journal-title":"Sci. Total Environ."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"918","DOI":"10.3390\/antibiotics9120918","article-title":"Global trends in antimicrobial use in food animals from 2017 to 2030","volume":"9","author":"Tiseo","year":"2020","journal-title":"Antibiotics"},{"key":"ref55","doi-asserted-by":"publisher","first-page":"7465","DOI":"10.1111\/1462-2920.15632","article-title":"The poultry pathogen Riemerella anatipestifer appears as a reservoir for Tet(X) tigecycline resistance","volume":"23","author":"Umar","year":"2021","journal-title":"Environ. Microbiol."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1089\/fpd.2014.1891","article-title":"Distribution of the multidrug resistance gene cfr in staphylococcus isolates from pigs, workers, and the environment of a hog market and a slaughterhouse in Guangzhou, China","volume":"12","author":"Wang","year":"2015","journal-title":"Foodborne Pathog. Dis."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"133781","DOI":"10.1016\/j.scitotenv.2019.133781","article-title":"Distribution characteristics of antibiotic resistant bacteria and genes in fresh and composted manures of livestock farms","volume":"695","author":"Wang","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"2474","DOI":"10.3389\/fmicb.2018.02474","article-title":"Metagenomic insights into the contribution of phages to antibiotic resistance in water samples related to swine feedlot wastewater treatment","volume":"9","author":"Wang","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"e0232937","DOI":"10.1371\/journal.pone.0232937","article-title":"New York state dairy farmers' perceptions of antibiotic use and resistance: a qualitative interview study","volume":"15","author":"Wemette","year":"2020","journal-title":"PLoS One"},{"key":"ref60","doi-asserted-by":"publisher","first-page":"144321","DOI":"10.1016\/j.scitotenv.2020.144321","article-title":"Cross-comparison of methods for quantifying antibiotic resistance in agricultural soils amended with dairy manure and compost","volume":"766","author":"Wind","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref61","doi-asserted-by":"publisher","first-page":"7445","DOI":"10.1111\/1462-2920.15425","article-title":"Co-production of Tet(X) and MCR-1, two resistance enzymes by a single plasmid","volume":"23","author":"Xu","year":"2021","journal-title":"Environ. Microbiol."},{"key":"ref62","doi-asserted-by":"publisher","first-page":"52346","DOI":"10.1074\/jbc.M409573200","article-title":"TetX is a flavin-dependent monooxygenase conferring resistance to tetracycline antibiotics","volume":"279","author":"Yang","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref63","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1186\/s12866-019-1548-x","article-title":"Comparative diversity of microbiomes and Resistomes in beef feedlots, downstream environments and urban sewage influent","volume":"19","author":"Zaheer","year":"2019","journal-title":"BMC Microbiol."},{"key":"ref64","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1016\/j.envint.2019.02.052","article-title":"Metagenomic insights into the distribution of antibiotic resistome between the gut-associated environments and the pristine environments","volume":"126","author":"Zeng","year":"2019","journal-title":"Environ. Int."},{"key":"ref65","doi-asserted-by":"publisher","first-page":"104912","DOI":"10.1016\/j.envint.2019.104912","article-title":"Transfer of antibiotic resistance from manure-amended soils to vegetable microbiomes","volume":"130","author":"Zhang","year":"2019","journal-title":"Environ. 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