{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:46:31Z","timestamp":1790948791506,"version":"4.1.0"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T00:00:00Z","timestamp":1675036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82002207"],"award-info":[{"award-number":["82002207"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Carbapenem-resistant <jats:italic>Klebsiella pneumoniae<\/jats:italic> is one of the primary bacterial pathogens that pose a significant threat to global public health because of the lack of available therapeutic options. Phage therapy shows promise as a potential alternative to current antimicrobial chemotherapies. In this study, we isolated a new <jats:italic>Siphoviridae<\/jats:italic> phage vB_KpnS_SXFY507 against KPC-producing <jats:italic>K. pneumoniae<\/jats:italic> from hospital sewage. It had a short latent period of 20\u2009min and a large burst size of 246 phages\/cell. The host range of phage vB_KpnS_SXFY507 was relatively broad. It has a wide range of pH tolerance and high thermal stability. The genome of phage vB_KpnS_SXFY507 was 53,122\u2009bp in length with a G\u2009+\u2009C content of 49.1%. A total of 81 open-reading frames (ORFs) and no virulence or antibiotic resistance related genes were involved in the phage vB_KpnS_SXFY507 genome. Phage vB_KpnS_SXFY507 showed significant antibacterial activity <jats:italic>in vitro<\/jats:italic>. The survival rate of <jats:italic>Galleria mellonella<\/jats:italic> larvae inoculated with <jats:italic>K. pneumoniae<\/jats:italic> SXFY507 was 20%. The survival rate of <jats:italic>K. pneumonia<\/jats:italic>-infected <jats:italic>G. mellonella<\/jats:italic> larvae was increased from 20 to 60% within 72\u2009h upon treatment with phage vB_KpnS_SXFY507. In conclusion, these findings indicate that phage vB_KpnS_SXFY507 has the potential to be used as an antimicrobial agent for the control of <jats:italic>K. pneumoniae<\/jats:italic>.<\/jats:p>","DOI":"10.3389\/fmicb.2023.1081715","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T04:51:20Z","timestamp":1675054280000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":19,"title":["Characterization and genome analysis of phage vB_KpnS_SXFY507 against Klebsiella pneumoniae and efficacy assessment in Galleria mellonella larvae"],"prefix":"10.3389","volume":"14","author":[{"given":"Jiao","family":"Feng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liting","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyong","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changxin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,1,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1272","DOI":"10.1016\/j.jmb.2007.12.041","article-title":"The ATPase domain of the large terminase protein, gp17, from bacteriophage T4 binds DNA: implications to the DNA packaging mechanism","volume":"376","author":"Alam","year":"2008","journal-title":"J. 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