{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:18:59Z","timestamp":1787026739803,"version":"build-2736575974"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T00:00:00Z","timestamp":1679443200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Glutathione peroxidase is abundant in eukaryotes as an important antioxidant enzyme. However, prokaryotic glutathione peroxidase has not been thoroughly studied.\n                      <jats:italic>Listeria monocytogenes<\/jats:italic>\n                      is a facultative intracellular pathogen that is capable of causing listeriosis in animals as well as humans. Despite the fact that\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      encodes a putative glutathione peroxidase, GSH-Px (encoded by\n                      <jats:italic>lmo0983<\/jats:italic>\n                      )), the functions of the enzyme are still unknown. Here we revealed the unusual roles of\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      GSH-Px in bacterial antioxidants and pathogenicity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      Lm850658 was taken as the parental strain to construct the gsh-px deletion strain and related complement strain. The effect of the gsh-px gene on the resistance of\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      to oxidative stress was determined by measuring the concentrations of glutathione and assaying the stress survival rates under different oxidative conditions. In addition, the pathogenicity of\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      was determined by cellular adhesion and invasion assays and mice virulence tests, and the expression of virulence factors was determined by Western blot.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Deficiency of GSH-Px not only increased glutathione concentrations in L. monocytogenes but also enhanced its resistance to oxidative stress when exposed to copper and iron ions. In addition, the absence of gsh-px significantly improved the adhesion and invasion efficiency of\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      to Caco-2 cells. More importantly,\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      lacking GSH-Px could colonize and proliferate more efficiently in mice livers and spleens, enhancing the pathogenicity of L. monocytogenes by increasing the expression of virulence factors like InlA, InlB, and LLO.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      Taken together, we confirmed that GSH-Px of\n                      <jats:italic>L. monocytogenes<\/jats:italic>\n                      has a counter-intuitive effect on the antioxidant capacity and pathogenicity.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2023.1122623","type":"journal-article","created":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T01:38:40Z","timestamp":1679449120000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Destroying glutathione peroxidase improves the oxidative stress resistance and pathogenicity of Listeria monocytogenes"],"prefix":"10.3389","volume":"14","author":[{"given":"Yu","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiongyan","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuying","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,3,22]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"55","DOI":"10.3109\/10425179509074701","article-title":"Identification of a glutathione peroxidase homolog in Neisseria meningitidis","volume":"6","author":"Aho","year":"1995","journal-title":"DNA Seq."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"e1009819","DOI":"10.1371\/journal.ppat.1009819","article-title":"Detoxification of methylglyoxal by the glyoxalase system is required for glutathione availability and virulence activation in listeria monocytogenes","volume":"17","author":"Anaya-Sanchez","year":"2021","journal-title":"PLoS Pathog."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1016\/j.bbrc.2010.07.002","article-title":"The Escherichia coli btuE gene, encodes a glutathione peroxidase that is induced under oxidative stress conditions","volume":"398","author":"Arenas","year":"2010","journal-title":"Biochem. 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