{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:34:30Z","timestamp":1785760470967,"version":"3.56.0"},"reference-count":57,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T00:00:00Z","timestamp":1676851200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Cyanogenic glycosides (CNglcs) are bioactive plant products involving in plant defense against herbivores by virtue of their abilities to release toxic hydrogen cyanide (HCN). <jats:italic>Aspergillus niger<\/jats:italic> has been shown to be effective in producing <jats:italic>\u03b2<\/jats:italic>-glucosidase, which could degrade CNglcs. However, whether <jats:italic>A. niger<\/jats:italic> could remove CNglcs under ensiling conditions is still unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, we first investigated the HCN contents in ratooning sorghums for two years, then the sorghums were ensiled with or without the addition of <jats:italic>A. niger<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two years\u2019 investigation indicated that the contents of HCN in fresh ratooning sorghum were larger than 801\u2009mg\/kg FW (fresh weight), which could not be reduced by silage fermentation under safety threshold (200\u2009mg\/kg FW). <jats:italic>A. niger<\/jats:italic> could produce <jats:italic>\u03b2<\/jats:italic>-glucosidase over a range of pH and temperature, which degraded the CNglcs and removed the hydrogen cyanide (HCN) at early days of ratooning sorghum fermentation. The addition of <jats:italic>A. niger<\/jats:italic> (2.56\u2009\u00d7\u200910<jats:sup>7<\/jats:sup> CFU\/ml) altered the microbial community, increased bacterial diversity, improved the nutritive qualities, and reduced the HCN contents in ensiled ratooning sorghum lower than 100\u2009mg\/kg FW after 60\u2009days of fermentation. Overall, the addition of 150\u2009ml <jats:italic>A. niger<\/jats:italic> +\u200950\u2009ml sterile water per 3\u2009kg silage could efficiently remove CNglcs from ratooning sorghum silage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In conclusion, <jats:italic>A. niger<\/jats:italic> could produce <jats:italic>\u03b2<\/jats:italic>-glucosidase which degraded the CNglcs during the early days of fermentation, benefiting the ensiling process and improving the utilization of ratooning sorghum.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2023.1128057","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T06:29:51Z","timestamp":1676874591000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Effects of Aspergillus niger on cyanogenic glycosides removal and fermentation qualities of ratooning sorghum"],"prefix":"10.3389","volume":"14","author":[{"given":"Jianrong","family":"Zhai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingpeng","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfeng","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junfeng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenlan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cai","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjun","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,2,20]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1590\/1806-9061-2020-1258","article-title":"Effects of bile acids and lipase supplementation in low-energy diets on growth performance, fat digestibility and meat quality in broiler chickens","volume":"22","author":"Arshad","year":"2020","journal-title":"Braz. 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