{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T20:43:52Z","timestamp":1792874632980,"version":"4.5.0"},"reference-count":56,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T00:00:00Z","timestamp":1680134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>The <jats:italic>Vibrio cholerae,<\/jats:italic> a gram-negative bacterium, is the causative agent of cholera. Quorum sensing is a cell-to-cell communication that leads to gene expression, accumulation of signaling molecules, biofilm formation, and production of virulence factors. The quorum sensing pathway in <jats:italic>V. cholerae<\/jats:italic> is regulated by <jats:italic>lux<\/jats:italic>O, and biofilm formation and other virulence factors are positively controlled by <jats:italic>aph<\/jats:italic>A and negatively by <jats:italic>hap<\/jats:italic>R. Hence, targeting the global regulator <jats:italic>lux<\/jats:italic>O would be a promising approach to modulate the QS to curtail <jats:italic>V. cholerae<\/jats:italic> pathogenesis. The present study investigated the modulating activity of quercetin and naringenin on biofilm formation and quorum-sensing regulated phenotypes in <jats:italic>V. cholerae<\/jats:italic>. Then after we determined the anti-quorum sensing capability of phytomolecules against the model organism <jats:italic>Chromobacterium violaceum<\/jats:italic>. Also, we performed flow cytometry for live\/dead bacteria, MTT assay, CLSM, and growth curve analysis to determine their role as QS modulators rather than anti-bacterial. <jats:italic>V. cholerae<\/jats:italic> strains VC287 and N16961 formed thick biofilm. We observed a two-fold reduction in the expression of biofilm-associated genes comprising <jats:italic>gbpA, vpsA, rbmA<\/jats:italic>, and <jats:italic>mbaA<\/jats:italic> in the presence of phytomolecules indicating that phytomolecules modulate quorum sensing pathway rather than killing the bacteria. These phytomolecules were non-toxic and non-hemolytic and had anti-adhesion and anti-invasion properties. In addition, quercetin and naringenin were found to be highly effective compared to known quorum-sensing inhibitors terrein and furanone C-30. Thus, this study provides evidence that phytomolecules: quercetin and naringenin modulate the quorum-sensing pathway rather than killing the bacteria and can be used as an anti-quorum-sensing molecule for therapy against the pathogen.<\/jats:p>","DOI":"10.3389\/fmicb.2023.1133569","type":"journal-article","created":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T14:12:00Z","timestamp":1680185520000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":32,"title":["Attenuation of quorum sensing system and virulence in Vibrio cholerae by phytomolecules"],"prefix":"10.3389","volume":"14","author":[{"given":"Subhasree","family":"Saha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shifu","family":"Aggarwal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Durg Vijai","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,3,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1007\/s00203-020-02026-3","article-title":"Gene expressing analysis indicates the role of pyrogallol as a novel antibiofilm and antivirulence agent against Acinetobacter baumannii","volume":"203","author":"Abirami","year":"2021","journal-title":"Arch. 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