{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:18:40Z","timestamp":1787026720667,"version":"build-2736575974"},"reference-count":65,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T00:00:00Z","timestamp":1681344000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Pathogenic strains of\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      have been clearly identified as the causative agents of extraintestinal and diarrheal infections; however, the etiopathogenic role of\n                      <jats:italic>E. coli<\/jats:italic>\n                      in other conditions, including colorectal cancer, remains unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study aimed to characterize mucosal\n                      <jats:italic>E. coli<\/jats:italic>\n                      isolates (\n                      <jats:italic>n<\/jats:italic>\n                      = 246) from 61 neoplasia patients and 20 healthy controls for the presence of 35 genetic determinants encoding known virulence factors.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Virulence determinants encoding invasin (\n                      <jats:italic>ibeA<\/jats:italic>\n                      ), siderophore receptor (\n                      <jats:italic>iroN<\/jats:italic>\n                      ), S-fimbriae (\n                      <jats:italic>sfa<\/jats:italic>\n                      ), and genotoxin (\n                      <jats:italic>usp<\/jats:italic>\n                      ) were more prevalent among\n                      <jats:italic>E. coli<\/jats:italic>\n                      isolated from patients with neoplasia compared to the control group (\n                      <jats:italic>p<\/jats:italic>\n                      &amp;lt; 0.05). In addition, the prevalence of these virulence determinants was increased in more advanced neoplasia stages (\n                      <jats:italic>p<\/jats:italic>\n                      <jats:sub>adj<\/jats:sub>\n                      &amp;lt; 0.0125). Compared to patients with advanced colorectal adenoma and carcinoma, the\n                      <jats:italic>ibeA<\/jats:italic>\n                      gene was rarely found in the control group and among patients with non-advanced adenoma (\n                      <jats:italic>p<\/jats:italic>\n                      &amp;lt; 0.05), indicating its potential as the advanced-neoplasia biomarker. Patients with neoplasia frequently had\n                      <jats:italic>E. coli<\/jats:italic>\n                      strains with at least one of the abovementioned virulence factors, whereby specific combinations of these virulence factors were found.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      These findings suggest that\n                      <jats:italic>E. coli<\/jats:italic>\n                      strains isolated from patients with colorectal neoplasia possess several virulence factors, which could contribute to the development of neoplastic processes in the large intestine.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2023.1141619","type":"journal-article","created":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T00:39:56Z","timestamp":1681346396000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Escherichia coli from biopsies differ in virulence genes between patients with colorectal neoplasia and healthy controls"],"prefix":"10.3389","volume":"14","author":[{"given":"Juraj","family":"Bos\u00e1k","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darina","family":"Kohoutov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mat\u011bj","family":"Hrala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jitka","family":"K\u0159enov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Mor\u00e1vkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanislav","family":"Rejchrt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Bure\u0161","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"\u0160majs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,4,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1126\/science.1224820","article-title":"Intestinal inflammation targets cancer-inducing activity of the microbiota.","volume":"338","author":"Arthur","year":"2012","journal-title":"Science"},{"key":"B2","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1016\/j.micpath.2009.09.003","article-title":"Microcins and urovirulence in Escherichia coli.","volume":"47","author":"Azpiroz","year":"2009","journal-title":"Microb. 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