{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:18:41Z","timestamp":1787026721006,"version":"build-2736575974"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T00:00:00Z","timestamp":1684281600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003981","name":"Agenzia Spaziale Italiana","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003981","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002946","name":"Deutsches Zentrum f\u00fcr Luft- und Raumfahrt","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002946","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Cyanobacteria are gaining considerable interest as a method of supporting the long-term presence of humans on the Moon and settlements on Mars due to their ability to produce oxygen and their potential as bio-factories for space biotechnology\/synthetic biology and other applications. Since many unknowns remain in our knowledge to bridge the gap and move cyanobacterial bioprocesses from Earth to space, we investigated cell division resumption on the rehydration of dried\n                    <jats:italic>Chroococcidiopsis<\/jats:italic>\n                    sp. CCMEE 029 accumulated DNA damage while exposed to space vacuum, Mars-like conditions, and Fe-ion radiation. Upon rehydration, the monitoring of the\n                    <jats:italic>ftsZ<\/jats:italic>\n                    gene showed that cell division was arrested until DNA damage was repaired, which took 48 h under laboratory conditions. During the recovery, a progressive DNA repair lasting 48 h of rehydration was revealed by PCR-stop assay. This was followed by overexpression of the ftsZ gene, ranging from 7.5- to 9-fold compared to the non-hydrated samples. Knowing the time required for DNA repair and cell division resumption is mandatory for deep-space experiments that are designed to unravel the effects of reduced\/microgravity on this process. It is also necessary to meet mission requirements for dried-sample implementation and real-time monitoring upon recovery. Future experiments as part of the lunar exploration mission Artemis and the lunar gateway station will undoubtedly help to move cyanobacterial bioprocesses beyond low Earth orbit. From an astrobiological perspective, these experiments will further our understanding of microbial responses to deep-space conditions.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2023.1150224","type":"journal-article","created":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T01:33:27Z","timestamp":1684287207000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Enabling deep-space experimentations on cyanobacteria by monitoring cell division resumption in dried Chroococcidiopsis sp. 029 with accumulated DNA damage"],"prefix":"10.3389","volume":"14","author":[{"given":"Claudia","family":"Fagliarone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Mosca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgia","family":"Di Stefano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Leuko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Moeller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elke","family":"Rabbow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Rettberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Billi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,5,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3389\/fspas.2021.711550","article-title":"Towards a biomanufactory on Mars","author":"Berliner","year":"2021","journal-title":"Front. Astron. Space Sci"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/BF03178322","article-title":"Loss of topological relationships in a Pleurocapsalean cyanobacterium (Chroococcidiopsis sp.) with partially inactivated","volume":"59","author":"Billi","year":"2009","journal-title":"ftsZ. Ann. Microbiol"},{"key":"B3","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1017\/S1473550420000300","article-title":"Exploiting a perchlorate-tolerant desert cyanobacterium to support bacterial growth for in situ resource utilization on Mars","volume":"20","author":"Billi","year":"2021","journal-title":"Int. J. Astrobiol"},{"key":"B4","doi-asserted-by":"publisher","first-page":"4053","DOI":"10.1128\/AEM.64.10.4053-4056.1998","article-title":"A method for DNA extraction from the desert cyanobacterium Chroococcidiopsis and its application to identification of ftsZ","volume":"64","author":"Billi","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1008","DOI":"10.1089\/ast.2018.1900","article-title":"Dried biofilms of desert strains of Chroococcidiopsis survived prolonged exposure to space and Mars-like conditions in low Earth orbit","volume":"19","author":"Billi","year":"2019","journal-title":"Astrobiology"},{"key":"B6","doi-asserted-by":"publisher","first-page":"1809","DOI":"10.3389\/fmicb.2016.01809","article-title":"Comparative genomics of DNA recombination and repair in cyanobacteria: biotechnological implications","volume":"7","author":"Cassier-Chauvat","year":"2016","journal-title":"Front. Microbiol"},{"key":"B7","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1111\/1751-7915.13927","article-title":"Bridging the gap between microbial limits and extremes in space: Space microbial biotechnology in the next 15years","volume":"15","author":"Cockell","year":"2022","journal-title":"Microb. Biotechnol"},{"key":"B8","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1089\/ast.2019.0625","article-title":"EXPOSE-R2 on the International Space Station (2014-2016): Results from the PSS and BOSS astrobiology experiments","volume":"19","author":"Cottin","year":"2019","journal-title":"Astrobiology"},{"key":"B9","first-page":"1","volume-title":"NASA's Gateway: An Update on Progress and Plans for Extending Human Presence to Cislunar Space","author":"Crusan","year":"2019"},{"key":"B10","doi-asserted-by":"publisher","first-page":"1475","DOI":"10.1002\/2016SW001580","article-title":"Overview of the ISS radiation environment observed during the ESA EXPOSE-R2 mission in 2014\u20132016","volume":"15","author":"Dachev","year":"2017","journal-title":"Space Weather"},{"key":"B11","doi-asserted-by":"publisher","first-page":"31","DOI":"10.3389\/fspas.2020.00031","article-title":"Biomarker preservation and survivability under and Mars-like UV flux of a desert cyanobacterium capable of trehalose and sucrose accumulation","volume":"7","author":"Fagliarone","year":"2020","journal-title":"Front. Astron. Space Sci."},{"key":"B12","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1128\/MMBR.00016-09","article-title":"Space microbiology","volume":"74","author":"Horneck","year":"2010","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a012674","article-title":"DNA damage responses in prokaryotes: Regulating gene expression, modulating growth patterns, and manipulating replication fork","author":"Kreuzer","year":"2013","journal-title":"Cold Spring Harb. Perspect. Biol"},{"key":"B14","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1016\/j.bbrc.2004.04.129","article-title":"Evidences showing ultraviolet-B radiation-induced damage of DNA in cyanobacteria and its detection by PCR assay","volume":"318","author":"Kumar","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"B15","doi-asserted-by":"publisher","first-page":"107946","DOI":"10.1016\/j.biotechadv.2022.107946","article-title":"Cyanobacteria and microalgae in supporting human habitation on Mars","volume":"59","author":"Mapstone","year":"2022","journal-title":"Biotechnol. Adv"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1089\/ast.2019.2068","article-title":"BioSentinel: a biological cubesat for deep space exploration","author":"Massaro Tieze","year":"2020","journal-title":"Astrobiology Apr"},{"key":"B17","doi-asserted-by":"publisher","first-page":"923","DOI":"10.3389\/fmicb.2020.00923","article-title":"Molecular mechanisms of microbial survivability in outer space: a systems biology approach","volume":"11","author":"Milojevic","year":"2020","journal-title":"Front. Microbiol"},{"key":"B18","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1089\/ast.2016.1571","article-title":"STARLIFE\u2014An international campaign to study the role of galactic cosmic radiation in astrobiological model systems","volume":"17","author":"Moeller","year":"2017","journal-title":"Astrobiology"},{"key":"B19","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1089\/ast.2020.2359","article-title":"Revival of anhydrobiotic cyanobacterium biofilms exposed to space vacuum and prolonged dryness: Implications for future missions beyond low Earth orbit","volume":"21","author":"Mosca","year":"2021","journal-title":"Astrobiology"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1017\/S1473550422000131","article-title":"Role of DNA repair pathways in the recovery of a dried, radioresistant cyanobacterium exposed to high-LET radiation: implications for the habitability of Mars","author":"Mosca","year":"2022","journal-title":"Int J Astrobiol"},{"key":"B21","doi-asserted-by":"publisher","first-page":"2312","DOI":"10.3389\/fmicb.2019.02312","article-title":"Over-expression of UV-damage DNA repair genes and ribonucleic acid persistence contribute to the resilience of dried biofilms of the desert cyanobacetrium Chroococcidiopsis exposed to Mars-like UV flux and long-term desiccation","volume":"10","author":"Mosca","year":"2019","journal-title":"Front. Microbiol"},{"key":"B22","doi-asserted-by":"publisher","first-page":"8437","DOI":"10.1038\/s41598-022-12631-5","article-title":"Absence of increased genomic variants in the cyanobacterium Chroococcidiopsis exposed to Mars-like conditions outside the space station","volume":"12","author":"Napoli","year":"2022","journal-title":"Sci. Rep"},{"key":"B23","doi-asserted-by":"publisher","first-page":"1533","DOI":"10.3389\/fmicb.2017.01533","article-title":"EXPOSE-R2: the astrobiological ESA mission on board of the International Space Station","volume":"8","author":"Rabbow","year":"2017","journal-title":"Front. Microbiol."},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-111-1-1","article-title":"Generic assignments, strain histories and properties of pure cultures of cyanobacteria","author":"Rippka","year":"1979","journal-title":"J. Gen. Microbiol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"1158","DOI":"10.1042\/BST20160067","article-title":"Synthetic biology meets bioprinting: enabling technologies for humans on Mars (and Earth)","volume":"44","author":"Rothschild","year":"2016","journal-title":"Biochem. Soc. Trans"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1089\/ast.2019.2073","article-title":"BioSentinel: long-term Saccharomyces cerevisiae preservation for a deep space biosensor mission","author":"Santa Maria","year":"2020","journal-title":"Astrobiology"},{"key":"B27","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/0273-1177(94)90478-2","article-title":"Influence of thiols and oxygen on the survival of gamma-irradiated plasmid DNA and cells","volume":"14","author":"Schulte-Frohlinde","year":"1994","journal-title":"Adv. Space Res"},{"key":"B28","author":"Smith","year":"2020","journal-title":"The Artemis Program: An Overview of NASA's Activities to Return Humans to the Moon"},{"key":"B29","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1089\/ast.2015.1450","article-title":"Evaluation of the resistance of Chroococcidiopsis spp. to sparsely and densely ionizing irradiation","volume":"17","author":"Verseux","year":"2017","journal-title":"Astrobiology"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2023.1150224\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T01:33:38Z","timestamp":1684287218000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2023.1150224\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,17]]},"references-count":29,"alternative-id":["10.3389\/fmicb.2023.1150224"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2023.1150224","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,17]]},"article-number":"1150224"}}