{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:05:11Z","timestamp":1791839111921,"version":"4.3.4"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T00:00:00Z","timestamp":1683849600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82273752"],"award-info":[{"award-number":["82273752"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81620108026"],"award-info":[{"award-number":["81620108026"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Osteoarthritis (OA) is a kind of chronic, degenerative disorder with unknown causes. In this study, we aimed to improve our understanding of the gut microbiota profile in patients with knee OA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>16S rDNA gene sequencing was performed to detect the gut microbiota in fecal samples collected from the patients with OA (<jats:italic>n<\/jats:italic>\u2009=\u200932) and normal control (NC, <jats:italic>n<\/jats:italic>\u2009=\u200957). Then the metagenomic sequencing was used to identify the genes or functions linked with gut microbial changes at the species level in the fecal samples from patients with OA and NC groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The Proteobacteria was identified as dominant bacteria in OA group. We identified 81 genera resulted significantly different in abundance between OA and NC. The abundance of <jats:italic>Agathobacter<\/jats:italic>, <jats:italic>Ruminococcus<\/jats:italic>, <jats:italic>Roseburia<\/jats:italic>, <jats:italic>Subdoligranulum<\/jats:italic>, and <jats:italic>Lactobacillus<\/jats:italic> showed significant decrease in the OA compared to the NC. The abundance of genera <jats:italic>Prevotella_7<\/jats:italic>, <jats:italic>Clostridium<\/jats:italic>, <jats:italic>Flavonifractor<\/jats:italic> and <jats:italic>Klebsiella<\/jats:italic> were increasing in the OA group, and the families <jats:italic>Lactobacillaceae<\/jats:italic>, <jats:italic>Christensenellaceae<\/jats:italic>, <jats:italic>Clostridiaceae_1<\/jats:italic> and <jats:italic>Acidaminococcaceae<\/jats:italic> were increasing in the NC. The metagenomic sequencing showed that the abundance of <jats:italic>Bacteroides stercoris<\/jats:italic>, <jats:italic>Bacteroides vulgatus<\/jats:italic> and <jats:italic>Bacteroides uniformis<\/jats:italic> at the species level were significantly decreasing in the OA, and the abundance of <jats:italic>Escherichia coli<\/jats:italic>, <jats:italic>Klebsiella pneumoniae<\/jats:italic>, <jats:italic>Shigella flexneri<\/jats:italic> and <jats:italic>Streptococcus salivarius<\/jats:italic> were significantly increased in OA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The results of our study interpret a comprehensive profile of the gut microbiota in patients with knee OA and offer the evidence that the cartilage-gut-microbiome axis could play a crucial role in underlying the mechanisms and pathogenesis of OA.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2023.1153424","type":"journal-article","created":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T05:29:56Z","timestamp":1683869396000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":24,"title":["Altered gut microbiome profile in patients with knee osteoarthritis"],"prefix":"10.3389","volume":"14","author":[{"given":"Xi","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanli","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feihong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sijie","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feiyu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaowei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruitian","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minhan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Ning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmou","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiong","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,5,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1007\/s11908-013-0335-3","article-title":"Rheumatic fever and post-group a streptococcal arthritis in children","volume":"15","author":"Barash","year":"2013","journal-title":"Curr. Infect. Dis. Rep."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1111\/j.1462-5822.2007.01043.x","article-title":"Borrelia burgdorferi BBB07 interaction with integrin alpha3beta1 stimulates production of pro-inflammatory mediators in primary human chondrocytes","volume":"10","author":"Behera","year":"2008","journal-title":"Cell. Microbiol."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: high resolution sample inference from Illumina amplicon data","volume":"13","author":"Benjamin","year":"2016","journal-title":"Nat. Methods"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.joca.2012.11.012","article-title":"Osteoarthritis as an inflammatory disease (osteoarthritis is not osteoarthrosis!)","volume":"21","author":"Berenbaum","year":"2013","journal-title":"Osteoarthr. Cartil."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"e001037","DOI":"10.1136\/rmdopen-2019-001037","article-title":"Cartilage-gut-microbiome axis: a new paradigm for novel therapeutic opportunities in osteoarthritis","volume":"5","author":"Berthelot","year":"2019","journal-title":"RMD Open"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"4881","DOI":"10.1038\/s41467-019-12873-4","article-title":"Intestinal microbiome composition and its relation to joint pain and inflammation","volume":"10","author":"Boer","year":"2019","journal-title":"Nat. Commun."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"1612","DOI":"10.1016\/j.bbamcr.2013.12.011","article-title":"Protein selection and export via outer membrane vesicles","volume":"1843","author":"Bonnington","year":"2014","journal-title":"BBA-Mol. Cell. Res."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"620","DOI":"10.1038\/nrmicro3480","article-title":"Through the wall: extracellular vesicles in gram-positive bacteria, mycobacteria and fungi","volume":"13","author":"Brown","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1097\/BOR.0000000000000373","article-title":"Metabolic syndrome-associated osteoarthritis","volume":"29","author":"Courties","year":"2017","journal-title":"Curr. Opin. Rheumatol."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"314","DOI":"10.3109\/08830185.2014.917411","article-title":"Rheumatic fever, autoimmunity, and molecular mimicry: the streptococcal connection","volume":"33","author":"Cunningham","year":"2014","journal-title":"Int. Rev. Immunol."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"916","DOI":"10.1136\/ard.2005.045724","article-title":"Do metabolic factors add to the effect of overweight on hand osteoarthritis? The Rotterdam study","volume":"66","author":"Dahaghin","year":"2007","journal-title":"Ann. Rheum. Dis."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"1111","DOI":"10.1002\/art.41210","article-title":"Identification of a human cartilage microbial DNA signature and associations with knee and hip osteoarthritis","volume":"72","author":"Dunn","year":"2018","journal-title":"Arthritis Rheumatol"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2","volume":"37","author":"Evan","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1097\/BOR.0000000000000681","article-title":"The gut microbiome-joint connection: implications in osteoarthritis","volume":"32","author":"Favazzo","year":"2020","journal-title":"Curr. Opin. Rheumatol."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1016\/j.joca.2018.07.020","article-title":"The effects of metabolic syndrome, obesity, and the gut microbiome on load-induced osteoarthritis","volume":"27","author":"Guss","year":"2019","journal-title":"Osteoarthr. Cartil."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"1638","DOI":"10.1002\/jbmr.2887","article-title":"Links between the microbiome and bone","volume":"31","author":"Hernandez","year":"2016","journal-title":"J. Bone Miner. Res."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1136\/annrheumdis-2019-216471","article-title":"Faecal microbiota transplantation from metabolically compromised human donors accelerates osteoarthritis in mice","volume":"79","author":"Huang","year":"2020","journal-title":"Ann. Rheum. Dis."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"1769","DOI":"10.1016\/j.joca.2016.05.008","article-title":"Both systemic and local lipopolysaccharide (LPS) burden are associated with knee OA severity and inflammation","volume":"24","author":"Huang","year":"2016","journal-title":"Osteoarthr. Cartil."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"1403","DOI":"10.1002\/art.39555","article-title":"Genome-wide DNA methylation study identifies significant Epigenomic changes in osteoarthritic subchondral bone and similarity to overlying cartilage","volume":"68","author":"Jeffries","year":"2016","journal-title":"Arthritis Rheumatol."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1016\/j.berh.2014.01.004","article-title":"The epidemiology of osteoarthritis","volume":"28","author":"Johnson","year":"2014","journal-title":"Best Pract. Res. Clin. Rheumatol."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"1138","DOI":"10.1089\/cmb.2017.0096","article-title":"Cut primers: a new tool for accurate cutting of primers from reads of targeted next generation sequencing","volume":"24","author":"Kechin","year":"2017","journal-title":"J. Comput. Biol."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"1481","DOI":"10.1016\/j.cell.2017.11.024","article-title":"Our gut microbiome: the evolving inner self","volume":"171","author":"Kundu","year":"2017","journal-title":"Cells"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. 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Cartil."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1186\/s13073-016-0307-y","article-title":"The healthy human microbiome","volume":"8","author":"Lloyd-Price","year":"2016","journal-title":"Genome Med."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/j.joca.2019.10.002","article-title":"The association of plasma fatty acids with hand and knee osteoarthritis: the NEO study","volume":"28","author":"Loef","year":"2020","journal-title":"Osteoarthr. Cartil."},{"key":"ref01","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1038\/ismej.2015.131","article-title":"Experimental insights into the importance of aquatic bacterial community composition to the degradation of dissolved organic matter","volume":"10","author":"Logue","year":"2016","journal-title":"ISME J."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"949","DOI":"10.1093\/rheumatology\/keh225","article-title":"Poststreptococcal reactive arthritis: what is it and how do we know?","volume":"43","author":"Mackie","year":"2004","journal-title":"Rheumatology"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"2054","DOI":"10.1001\/jama.1977.03280200066025","article-title":"Osteomyelitis caused by Beta-hemolytic Streptococcus group-B","volume":"238","author":"Mcguire","year":"1977","journal-title":"JAMA"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1042\/CS20120073","article-title":"Does endotoxaemia contribute to osteoarthritis in obese patients?","volume":"123","author":"Metcalfe","year":"2012","journal-title":"Clin. Sci."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"Kegg: kyoto encyclopedia of genes and genomes","volume":"28","author":"Minoru","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"D199","DOI":"10.1093\/nar\/gkt1076","article-title":"Data, information, knowledge and principle: back to metabolism in kegg","volume":"42","author":"Minoru","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"O33","DOI":"10.1111\/1469-0691.12302","article-title":"Streptococcal vertebral osteomyelitis: multiple faces of the same disease","volume":"20","author":"Murillo","year":"2014","journal-title":"Clin. Microbiol. Infect."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"7421","DOI":"10.1073\/pnas.1201193109","article-title":"Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4\/MD-2","volume":"109","author":"Ohto","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"1420","DOI":"10.1093\/bioinformatics\/bts174","article-title":"IDBA-UD: a de novo assembler for single-cell and metagenomic sequencing data with highly uneven depth","volume":"28","author":"Peng","year":"2012","journal-title":"Bioinformatics"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1038\/nrmicro2974","article-title":"The gut microbiota - masters of host development and physiology","volume":"11","author":"Sommer","year":"2013","journal-title":"Nat. Rev. Microbiol."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"e01134","DOI":"10.1016\/j.heliyon.2019.e01134","article-title":"A correlation between intestinal microbiota dysbiosis and osteoarthritis","volume":"5","author":"Szychlinska","year":"2019","journal-title":"Heliyon"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1038\/nrcardio.2012.197","article-title":"Revisiting the pathogenesis of rheumatic fever and carditis","volume":"10","author":"Tandon","year":"2013","journal-title":"Nat. Rev. Cardiol."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"14305","DOI":"10.1038\/s41598-018-32675-w","article-title":"Detection and characterization of bacterial nucleic acids in culture-negative synovial tissue and fluid samples from rheumatoid arthritis or osteoarthritis patients","volume":"8","author":"Zhao","year":"2018","journal-title":"Sci. Rep."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"e132","DOI":"10.1093\/nar\/gkq275","article-title":"An initio gene identification in metagenomic sequences","volume":"38","author":"Zhu","year":"2010","journal-title":"Nucleic Acids Res."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2023.1153424\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T05:30:01Z","timestamp":1683869401000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2023.1153424\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,12]]},"references-count":42,"alternative-id":["10.3389\/fmicb.2023.1153424"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2023.1153424","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,12]]},"article-number":"1153424"}}