{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:03:14Z","timestamp":1753891394769,"version":"3.41.2"},"reference-count":61,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T00:00:00Z","timestamp":1680048000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006360","name":"Federal Ministry for Economic Affairs and Energy","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006360","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Given the worldwide increasing prevalence of human <jats:italic>Campylobacter jejuni<\/jats:italic> infections and the emergence of multi-drug resistant enteropathogenic strains, antibiotic-independent approaches applying non-toxic natural compounds for the treatment and prophylaxis of campylobacteriosis appear utmost desirable. In our placebo-controlled intervention study, we surveyed potential disease-alleviating including anti-pathogenic and immune-modulatory effects upon prophylactic oral application of lemon-essential oil (LEM-EO) and coriander-essential oil (COR-EO) in acute experimental campylobacteriosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Therefore, secondary abiotic IL-10<jats:sup>\u2212\/\u2212<\/jats:sup> mice were orally challenged with either LEM-EO or COR-EO starting seven days prior to peroral <jats:italic>C. jejuni<\/jats:italic> infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and discussion<\/jats:title><jats:p>Six days post-infection, slightly lower pathogen loads were assessed in the colon of mice from the LEM-EO as opposed to the COR-EO cohort if compared to placebo counterparts. Prophylactic application of both EOs improved the clinical outcome of acute campylobacteriosis which was paralleled by less distinct pathogen-induced colonic epithelial cell apoptosis. Moreover, mice subjected to LEM-EO and COR-EO prophylaxis displayed lower colonic numbers of macrophages\/monocytes and of T lymphocytes, respectively, whereas in both verum groups, basal IL-6 and IFN-\u03b3 concentrations were measured in mesenteric lymph nodes on day 6 post-infection. The oral challenge with either EOs resulted in diminished secretion of distinct pro-inflammatory mediators in the kidney as well as serum samples derived from the infected mice. In conclusion, the results from our preclinical <jats:italic>in vivo<\/jats:italic> study provide evidence that LEM-EO and COR-EO constitute promising prophylactic measures to prevent severe campylobacteriosis which may help to reduce the risk for development of post-infectious sequelae in <jats:italic>C. jejuni<\/jats:italic> infected individuals.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2023.1154407","type":"journal-article","created":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T07:24:18Z","timestamp":1680074658000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Disease alleviating effects following prophylactic lemon and coriander essential oil treatment in mice with acute campylobacteriosis"],"prefix":"10.3389","volume":"14","author":[{"given":"Soraya","family":"Mousavi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis","family":"Weschka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Bereswill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus M.","family":"Heimesaat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2023,3,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"41540","DOI":"10.1007\/s11356-020-10066-x","article-title":"Protective effects of Citrus limonia oil against cisplatin-induced nephrotoxicity","volume":"27","author":"Abdel-Daim","year":"2020","journal-title":"Environ. 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