{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T16:34:29Z","timestamp":1787589269613,"version":"build-2736575974"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T00:00:00Z","timestamp":1681689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    <jats:italic>Azoarcus<\/jats:italic>\n                    sp. DN11 was previously isolated from gasoline-contaminated groundwater as an anaerobic benzene-degrading bacterium. Genome analysis of strain DN11 revealed that it contained a putative\n                    <jats:italic>idr<\/jats:italic>\n                    gene cluster (\n                    <jats:italic>\n                      idrABP\n                      <jats:sub>1<\/jats:sub>\n                      P\n                      <jats:sub>2<\/jats:sub>\n                    <\/jats:italic>\n                    ), which was recently found to be involved in bacterial iodate (IO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    ) respiration. In this study, we determined if strain DN11 performed iodate respiration and assessed its potential use to remove and sequester radioactive iodine (\n                    <jats:sup>129<\/jats:sup>\n                    I) from subsurface contaminated aquifers. Strain DN11 coupled acetate oxidation to iodate reduction and grew anaerobically with iodate as the sole electron acceptor. The respiratory iodate reductase (Idr) activity of strain DN11 was visualized on non-denaturing gel electrophoresis, and liquid chromatography\u2013tandem mass spectrometry analysis of the active band suggested the involvement of IdrA, IdrP\n                    <jats:sub>1<\/jats:sub>\n                    , and IdrP\n                    <jats:sub>2<\/jats:sub>\n                    in iodate respiration. The transcriptomic analysis also showed that\n                    <jats:italic>idrA<\/jats:italic>\n                    ,\n                    <jats:italic>\n                      idrP\n                      <jats:sub>1<\/jats:sub>\n                    <\/jats:italic>\n                    , and\n                    <jats:italic>\n                      idrP\n                      <jats:sub>2<\/jats:sub>\n                    <\/jats:italic>\n                    expression was upregulated under iodate-respiring conditions. After the growth of strain DN11 on iodate, silver-impregnated zeolite was added to the spent medium to remove iodide from the aqueous phase. In the presence of 200\u2009\u03bcM iodate as the electron acceptor, more than 98% of iodine was successfully removed from the aqueous phase. These results suggest that strain DN11 is potentially helpful for bioaugmentation of\n                    <jats:sup>129<\/jats:sup>\n                    I-contaminated subsurface aquifers.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2023.1162788","type":"journal-article","created":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T00:55:05Z","timestamp":1681692905000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Iodate respiration by Azoarcus sp. DN11 and its potential use for removal of radioiodine from contaminated aquifers"],"prefix":"10.3389","volume":"14","author":[{"given":"Seiya","family":"Sasamura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiko","family":"Ohnuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naofumi","family":"Kozai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seigo","family":"Amachi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,4,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1264\/jsme2.ME08548","article-title":"Microbial contribution to global iodine cycling: volatilization, accumulation, reduction, oxidation, and sorption of iodine","volume":"23","author":"Amachi","year":"2008","journal-title":"Microbes Environ."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"2718","DOI":"10.1128\/AEM.67.6.2718-2722.2001","article-title":"Bacteria mediate methylation of iodine in marine and terrestrial environments","volume":"67","author":"Amachi","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1007\/s00248-004-0056-0","article-title":"Isolation of iodide-oxidizing bacteria from iodine-rich natural gas brines and seawaters","volume":"49","author":"Amachi","year":"2005","journal-title":"Microbial Ecol."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"20200824","DOI":"10.1098\/rspa.2020.0824","article-title":"Marine iodine emissions in a changing world","volume":"477","author":"Carpenter","year":"2021","journal-title":"Proc. R. Soc. A"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1038\/s41572-022-00357-7","article-title":"Hypothyroidism","volume":"8","author":"Chaker","year":"2022","journal-title":"Nat. Rev. Dis. Primers."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"3886","DOI":"10.3390\/nu14193886","article-title":"Thyroidal and extrathyroidal requirements for iodine and selenium: a combined evolutionary and (patho)physiological approach","volume":"14","author":"Dijck-Brouwer","year":"2022","journal-title":"Nutrients"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1016\/0016-7037(85)90027-4","article-title":"Natural iodine-129 as an environmental tracer","volume":"49","author":"Fabryka-Martin","year":"1985","journal-title":"Geochim. Cosmochim. Acta"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1016\/j.apgeochem.2015.09.013","article-title":"Iodine and human health, the role of environmental geochemistry and diet, a review","volume":"63","author":"Fuge","year":"2015","journal-title":"Appl. Geochem."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"2287","DOI":"10.1080\/10643389.2013.828273","article-title":"Radioiodine biogeochemistry and prevalence in groundwater","volume":"44","author":"Kaplan","year":"2014","journal-title":"Crit. Rev. Environ. Sci. Technol."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"576","DOI":"10.1016\/j.envint.2019.02.070","article-title":"Iodine speciation in a silver-amended cementitious system","volume":"126","author":"Kaplan","year":"2019","journal-title":"Environ. Int."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"6222","DOI":"10.1021\/es062842p","article-title":"Degradative capacities and bioaugmentation potential of an anaerobic benzene-degrading bacterium strain DN11","volume":"41","author":"Kasai","year":"2007","journal-title":"Environ. Sci. Technol."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"3586","DOI":"10.1128\/AEM.72.5.3586-3592.2006","article-title":"RNA-based stable isotope probing and isolation of anaerobic benzene-degrading bacteria from gasoline-contaminated groundwater","volume":"72","author":"Kasai","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"106017","DOI":"10.1016\/j.jenvrad.2019.106017","article-title":"Iodine immobilization by silver-impregnated granular activated carbon in cementitious systems","author":"Li","year":"2019","journal-title":"J. Environ. Radioact."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"570","DOI":"10.1080\/01490451.2018.1430189","article-title":"Iodate reduction by Shewanella oneidensis does not involve nitrate reductase","volume":"35","author":"Mok","year":"2018","journal-title":"Geomicrobiol J."},{"key":"ref150","doi-asserted-by":"publisher","first-page":"e00829-20","DOI":"10.1128\/AEM.00829-20","article-title":"Possible involvement of a tetrathionate reductase homolog in dissimilatory arsenate reduction by Anaeromyxobacter sp. PSR-1","volume":"86","author":"Muramatsu","year":"2020","journal-title":"Appl. Environ. Microbiol."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"466","DOI":"10.1016\/j.scitotenv.2019.07.146","article-title":"A review of the behavior of radioiodine in the subsurface at two DOE sites","volume":"691","author":"Neeway","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"632","DOI":"10.1038\/nature00775","article-title":"Marine aerosol formation from biogenic iodine emissions","volume":"417","author":"O'Dowd","year":"2002","journal-title":"Nature"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"3857","DOI":"10.1016\/j.scitotenv.2011.05.018","article-title":"Factors controlling mobility of 127I and 129I species in an acidic groundwater plume at the Savannah River site","volume":"409","author":"Otosaka","year":"2011","journal-title":"Sci. Total Environ."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1038\/s41396-021-01034-5","article-title":"Genetic and phylogenetic analysis of dissimilatory iodate-reducing bacteria identifies potential niches across theworld's oceans","volume":"16","author":"Reyes-Umana","year":"","journal-title":"ISME J."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"804181","DOI":"10.3389\/fmicb.2021.804181","article-title":"Isolation of a dissimilatory iodate-reducing Aromatoleum sp. from a freshwater creek in the San Francisco Bay Area","volume":"12","author":"Reyes-Umana","year":"","journal-title":"Front. Microbiol."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"850","DOI":"10.1021\/ac950914h","article-title":"Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels","volume":"68","author":"Shevchenko","year":"1996","journal-title":"Anal. Chem."},{"key":"ref21","article-title":"The physical chemistry of seawater","volume-title":"Oceanography","author":"Sillen","year":"1961"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1007\/s00253-006-0522-3","article-title":"Rapid intrinsic biodegradation of benzene, toluene, and xylenes at the boundary of a gasoline-contaminated plume under natural attenuation","volume":"73","author":"Takahata","year":"2006","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref220","doi-asserted-by":"publisher","first-page":"e00763-19","DOI":"10.1128\/AEM.00763-19","article-title":"Expression of genes and proteins involved in arsenic respiration and resistance in dissimilatory arsenate-reducing Geobacter sp. OR-1","volume":"85","author":"Tsuchiya","year":"2019","journal-title":"Appl. Environ. Microbiol."},{"key":"ref23","first-page":"45","article-title":"The marine geochemistry of iodine","volume":"4","author":"Wong","year":"1991","journal-title":"Rev. Aquat. Sci."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"2196","DOI":"10.1111\/1462-2920.14988","article-title":"A novel dimethylsulfoxide reductase family of molybdoenzyme, Idr, is involved in iodate respiration by pseudomonas sp. SCT","volume":"22","author":"Yamazaki","year":"2020","journal-title":"Environ. Microbiol."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/bs.aambs.2017.07.002","article-title":"Microbial transformation of iodine: from radioisotopes to iodine deficiency","volume":"101","author":"Yeager","year":"2017","journal-title":"Adv. Appl. Microbiol."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1007\/BF00475499","article-title":"Studies on the sorption of I\u2212 (iodide) and IO3\u2212 (iodate) onto andosols","volume":"63","author":"Yoshida","year":"1992","journal-title":"Water Air Soil Pollut."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"9635","DOI":"10.1021\/es401816e","article-title":"Iodine-129 and iodine-127 speciation in groundwater at the Hanford site, U.S.: iodate incorporation into calcite","volume":"47","author":"Zhang","year":"2013","journal-title":"Environ. Sci. Technol."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2023.1162788\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T00:55:09Z","timestamp":1681692909000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2023.1162788\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,17]]},"references-count":29,"alternative-id":["10.3389\/fmicb.2023.1162788"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2023.1162788","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,17]]},"article-number":"1162788"}}