{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T03:35:17Z","timestamp":1792899317336,"version":"4.5.0"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T00:00:00Z","timestamp":1690243200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2300400"],"award-info":[{"award-number":["2021YFC2300400"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Shanghai Natural Science Foundation","doi-asserted-by":"publisher","award":["20ZR1459500"],"award-info":[{"award-number":["20ZR1459500"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173864"],"award-info":[{"award-number":["82173864"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:italic>Candida albicans<\/jats:italic> is a commensal fungus that colonizes most healthy individuals\u2019 skin and mucosal surfaces but can also cause life-threatening invasive infections, particularly in immunocompromised patients. Despite antifungal treatment availability, drug resistance is increasing, and mortality rates remain unacceptably high. Heat shock protein Ssa1, a conserved member of the Hsp70 family in yeast, is a novel invasin that binds to host cell cadherins, induces host cell endocytosis, and enables <jats:italic>C. albicans<\/jats:italic> to cause maximal damage to host cells and induces disseminated and oropharyngeal disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>Here we discovered a mouse monoclonal antibody (mAb 13F4) that targeting <jats:italic>C. albicans<\/jats:italic> Ssa1 with high affinity (EC<jats:sub>50<\/jats:sub>\u2009=\u200939.78\u2009ng\/mL). mAb 13F4 prevented <jats:italic>C. albicans<\/jats:italic> from adhering to and invading human epithelial cells, displayed antifungal activity, and synergized with fluconazole in proof of concept <jats:italic>in vivo<\/jats:italic> studies. mAb 13F4 significantly prolonged the survival rate of the hematogenous disseminated candidiasis mice to 75%. We constructed a mAb 13F4 three-dimensional structure using homology modeling methods and found that the antigen-binding fragment (Fab) interacts with the Ssa1 N-terminus.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>These results suggest that blocking Ssa1 cell surface function may effectively control invasive <jats:italic>C. albicans<\/jats:italic> infections and provide a potential new treatment strategy for invasive fungal infections.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2023.1182914","type":"journal-article","created":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T17:10:45Z","timestamp":1690305045000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Ssa1-targeted antibody prevents host invasion by Candida albicans"],"prefix":"10.3389","volume":"14","author":[{"given":"Xi-Ran","family":"Qiu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen-Rui","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Wen","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Tong","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mao-Mao","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,7,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"2660","DOI":"10.1128\/AEM.70.5.2660-2666.2004","article-title":"Heat shock protein-mediated resistance to high hydrostatic pressure in Escherichia coli","volume":"70","author":"Aertsen","year":"2004","journal-title":"Appl. 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