{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T21:25:03Z","timestamp":1782163503413,"version":"3.54.5"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,7,6]],"date-time":"2023-07-06T00:00:00Z","timestamp":1688601600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Evidence from previous studies have implicated an important association between gut microbiota (GM) and Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome (ME\/CFS), but whether there is a definite causal relationship between GM and ME\/CFS has not been elucidated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>This study obtained instrumental variables of 211 GM taxa from the Genome Wide Association Study (GWAS), and mendelian randomization (MR) study was carried out to assess the effect of gut microbiota on ME\/CFS risk from UK Biobank GWAS (2076 ME\/CFS cases and 460,857 controls). Inverse variance weighted (IVW) was the primary method to analyze causality in this study, and a series of sensitivity analyses was performed to validate the robustness of the results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The inverse variance weighted (IVW) method indicated that genus <jats:italic>Paraprevotella<\/jats:italic> (OR:1.001, 95%CI:1.000\u20131.003, value of <jats:italic>p<\/jats:italic> &amp;lt; 0.05) and <jats:italic>Ruminococca- ceae_UCG_014<\/jats:italic> (OR 1.003, 95% CI 1.000 to 1.005, value of <jats:italic>p<\/jats:italic> &amp;lt; 0.05) were positively associated with ME\/CFS risk. Results from the weighted median method supported genus <jats:italic>Paraprevotella<\/jats:italic> (OR 1.003, 95% CI 1.000 to 1.005, value of <jats:italic>p<\/jats:italic> &amp;lt; 0.05) as a risk factor for ME\/CFS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study reveals a causal relationship between genus <jats:italic>paraprevotella<\/jats:italic>, genus <jats:italic>Ruminococcaceae_UCG_014<\/jats:italic> and ME\/CFS, and our findings provide novel insights for further elucidating the developmental mechanisms mediated by the gut microbiota of ME\/CFS.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2023.1190894","type":"journal-article","created":{"date-parts":[[2023,7,6]],"date-time":"2023-07-06T19:26:50Z","timestamp":1688671610000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Causal Effects between Gut Microbiome and Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome: A Two-Sample Mendelian Randomization Study"],"prefix":"10.3389","volume":"14","author":[{"given":"Gang","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honghao","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siran","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wangzi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dai","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongquan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houzhao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,7,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1001\/archinte.160.2.221","article-title":"Overlapping conditions among patients with chronic fatigue syndrome fibromyalgia, and temporomandibular disorder","volume":"160","author":"Aaron","year":"2000","journal-title":"Arch Intern Med."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1007\/s11306-016-1145-z","article-title":"The association of fecal microbiota and fecal, blood serum and urine metabolites in myalgic encephalomyelitis\/chronic fatigue syndrome","volume":"13","author":"Armstrong","year":"2017","journal-title":"Metabolomics"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1016\/j.chom.2015.04.004","article-title":"Dynamics and stabilization of the human gut microbiome during the first year of life","volume":"17","author":"B\u00e4ckhed","year":"2015","journal-title":"Cell Host Microbe"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.bbi.2011.06.016","article-title":"Chronic fatigue syndrome, the immune system and viral infection","volume":"26","author":"Bansal","year":"2012","journal-title":"Brain Behav. 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