{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T07:11:42Z","timestamp":1792048302823,"version":"4.4.0"},"reference-count":72,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,6,12]],"date-time":"2023-06-12T00:00:00Z","timestamp":1686528000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>This study aimed to identify and characterize novel siderophore-producing organisms capable of secreting high quantities of the iron-binding compounds. In the course of this, two not yet reported halophilic strains designated ATCHA<jats:sup>T<\/jats:sup> and ATCH28<jats:sup>T<\/jats:sup> were isolated from hypersaline, alkaline surface waters of Salar de Llamar\u00e1 and Laguna Lej\u00eda, respectively. The alkaline environment limits iron bioavailability, suggesting that native organisms produce abundant siderophores to sequester iron.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Both strains were characterized by polyphasic approach. Comparative analysis of the 16S rRNA gene sequences revealed their affiliation with the genus <jats:italic>Halomonas<\/jats:italic>. ATCHA<jats:sup>T<\/jats:sup> showed close similarity to <jats:italic>Halomonas salicampi<\/jats:italic> and <jats:italic>Halomonas vilamensis<\/jats:italic>, while ATCH28<jats:sup>T<\/jats:sup> was related closest to <jats:italic>Halomonas ventosae<\/jats:italic> and <jats:italic>Halomonas salina<\/jats:italic>. The ability of both strains to secrete siderophores was initially assessed using the chromeazurol S (CAS) liquid assay and subsequently further investigated through genomic analysis and NMR. Furthermore, the effect of various media components on the siderophore secretion by strain ATCH28<jats:sup>T<\/jats:sup> was explored.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The CAS assay confirmed the ability of both strains to produce iron-binding compounds. Genomic analysis of strain ATCHA<jats:sup>T<\/jats:sup> revealed the presence of a not yet reported NRPS-dependant gene cluster responsible for the secretion of siderophore. However, as only small amounts of siderophore were secreted, further investigations did not lie within the scope of this study. Via NMR and genomic analysis, strain ATCH28<jats:sup>T<\/jats:sup> has been determined to produce desferrioxamine E (DFOE). Although this siderophore is common in various terrestrial microorganisms, it has not yet been reported to occur within <jats:italic>Halomonas<\/jats:italic>, making strain ATCH28<jats:sup>T<\/jats:sup> the first member of the genus to produce a non-amphiphilic siderophore. By means of media optimization, the produced quantity of DFOE could be increased to more than 1000 \u00b5M.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Phenotypic and genotypic characteristics clearly differentiated both strains from other members of the genus <jats:italic>Halomonas<\/jats:italic>. Average nucleotide identity (ANI) values and DNA\u2013DNA relatedness indicated that the strains represented two novel species. Therefore, both species should be added as new representatives of the genus <jats:italic>Halomonas<\/jats:italic>, for which the designations <jats:italic>Halomonas llamarensis<\/jats:italic> sp. nov. (type strain ATCHA<jats:sup>T<\/jats:sup>\u2009=\u2009DSM 114476\u2009=\u2009LMG 32709) and <jats:italic>Halomonas gemina<\/jats:italic> sp. nov. (type strain ATCH28<jats:sup>T<\/jats:sup>\u2009=\u2009DSM 114418\u2009=\u2009LMG 32708) are proposed.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2023.1194916","type":"journal-article","created":{"date-parts":[[2023,6,12]],"date-time":"2023-06-12T04:32:07Z","timestamp":1686544327000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Halomonas gemina sp. nov. and Halomonas llamarensis sp. nov., two siderophore-producing organisms isolated from high-altitude salars of the Atacama Desert"],"prefix":"10.3389","volume":"14","author":[{"given":"Christian","family":"Hintersatz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shalini","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Antonio","family":"Rojas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerome","family":"Kretzschmar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean Ting-Shyang","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khushal","family":"Khambhati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Kutschke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Falk","family":"Lehmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijai","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohan","family":"Jain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Pollmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,6,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"2979","DOI":"10.3390\/MOLECULES25132979","article-title":"Novel south African rare Actinomycete Kribbella speibonae strain SK5: a prolific producer of hydroxamate siderophores including new dehydroxylated congeners","volume":"25","author":"Acquah","year":"2020","journal-title":"Molecules"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1007\/BF00369386","article-title":"Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria","volume":"12","author":"Alexander","year":"1991","journal-title":"Biol. Fertil. Soils"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"W29","DOI":"10.1093\/nar\/gkab335","article-title":"antiSMASH 6.0: improving cluster detection and comparison capabilities","volume":"49","author":"Blin","year":"2021","journal-title":"Nucl. Acids Res."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"739","DOI":"10.1134\/S0026261707060148","article-title":"Halomonas mongoliensis sp. nov. and Halomonas kenyensis sp. nov., new haloalkaliphilic denitrifiers capable of N2O reduction, isolated from soda lakes","volume":"76","author":"Boltyanskaya","year":"2007","journal-title":"Microbiology"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"1625","DOI":"10.1099\/00207713-51-5-1625\/","article-title":"Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium","volume":"51","author":"Bouchotroch","year":"2001","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"546","DOI":"10.3923\/PJBS.2010.546.550","article-title":"Optimization of culture medium to increase the production of desferrioxamine B (Desferal) in Streptomyces pilosus","volume":"13","author":"Chiani","year":"2010","journal-title":"Pakistan J. Biol. Sci."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1007\/S00253-008-1685-X\/","article-title":"Utilization of arylaliphatic nitriles by haloalkaliphilic Halomonas nitrilicus sp. nov. isolated from soda soils","volume":"81","author":"Chmura","year":"2008","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1099\/IJSEM.0.002516\/","article-title":"Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes","volume":"68","author":"Chun","year":"2018","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1007\/S007920050007","article-title":"Halomonas magadii sp. nov., a new member of the genus Halomonas, isolated from a soda lake of the east African Rift Valley","volume":"4","author":"Duckworth","year":"2000","journal-title":"Extremophiles"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"1792","DOI":"10.1093\/NAR\/GKH340","article-title":"MUSCLE: multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucl. Acids Res."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"783","DOI":"10.2307\/2408678","article-title":"Confidence limits on phylogenies: an approach using the bootstrap","volume":"39","author":"Felsenstein","year":"1985","journal-title":"Evolution"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"1183","DOI":"10.1007\/s00792-015-0790-x","article-title":"Structural characterization of amphiphilic siderophores produced by a soda lake isolate, Halomonas sp. SL01, reveals cysteine-, phenylalanine-and proline-containing head groups","volume":"19","author":"Figueroa","year":"2015","journal-title":"Extremophiles"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/S0723-2020(88)80043-2","article-title":"Halomonadaceae fam. Nov., a new family of the class Proteobacteria to accommodate the genera Halomonas and Deleya","volume":"11","author":"Franzmann","year":"1988","journal-title":"Syst. Appl. Microbiol."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"2467","DOI":"10.1271\/bbb.130597","article-title":"Heterologous production of desferrioxamines with a fusion biosynthetic gene cluster","volume":"77","author":"Fujita","year":"2013","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1556\/030.63.2016.029","article-title":"Optimization of desferrioxamine E production by Streptomyces parvulus","volume":"63","author":"G\u00e1ll","year":"2016","journal-title":"Acta Microbiol. Immunol. Hung."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"1723","DOI":"10.1099\/IJS.0.63114-0\/","article-title":"Halomonas organivorans sp. nov., a moderate halophile able to degrade aromatic compounds","volume":"54","author":"Garc\u00eda","year":"2004","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref19","author":"Geerlof","year":"2010"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"e0248385","DOI":"10.1371\/JOURNAL.PONE.0248385","article-title":"Characterization of a broadly specific cadaverine N-hydroxylase involved in desferrioxamine B biosynthesis in Streptomyces sviceus","volume":"16","author":"Giddings","year":"2021","journal-title":"PLoS One"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"1072","DOI":"10.1093\/BIOINFORMATICS\/BTT086","article-title":"QUAST: quality assessment tool for genome assemblies","volume":"29","author":"Gurevich","year":"2013","journal-title":"Bioinformatics"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"718","DOI":"10.1128\/JB.184.3.718-727.2002","article-title":"High-salinity-induced Iron limitation in Bacillus subtilis","volume":"184","author":"Hoffmann","year":"2002","journal-title":"J. Bacteriol."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"884","DOI":"10.1021\/NP800640H\/","article-title":"Loihichelins A-F, a suite of amphiphilic siderophores produced by the marine bacterium Halomonas LOB-5","volume":"72","author":"Homann","year":"2009","journal-title":"J. Nat. Prod."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/0167-7012(91)90049-V","article-title":"Improved method for phenotypical characterization of marine bacteria","volume":"13","author":"H\u00f8vik Hansen","year":"1991","journal-title":"J. Microbiol. Methods"},{"key":"ref25","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1016\/J.WATRES.2019.04.005","article-title":"Recovery of gallium from wafer fabrication industry wastewaters by desferrioxamine B and E using reversed-phase chromatography approach","volume":"158","author":"Jain","year":"2019","journal-title":"Water Res."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1007\/S10482-012-9832-X\/","article-title":"Halomonas cibimaris sp. nov., isolated from jeotgal, a traditional Korean fermented seafood","volume":"103","author":"Jeong","year":"2013","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1099\/ijs.0.02942-0","article-title":"Halomonas ventosae sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium","volume":"54","author":"Jos\u00e9 Mart\u00ednez-C\u00e1","year":"2004","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1007\/S10534-015-9827-Y\/","article-title":"Acyl peptidic siderophores: structures, biosyntheses and post-assembly modifications","volume":"28","author":"Kem","year":"2015","journal-title":"Biometals"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"609","DOI":"10.1007\/S12275-021-1231-4\/","article-title":"UBCG2: up-to-date bacterial core genes and pipeline for phylogenomic analysis","volume":"59","author":"Kim","year":"2021","journal-title":"J. Microbiol."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"2053","DOI":"10.1099\/IJSEM.0.001872","article-title":"ContEst16S: an algorithm that identifies contaminated prokaryotic genomes using 16S RNA gene sequences","volume":"67","author":"Lee","year":"2017","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"2027","DOI":"10.1099\/IJS.0.63616-0\/","article-title":"Halomonas taeanensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea","volume":"55","author":"Lee","year":"2005","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"4792","DOI":"10.1099\/ijsem.0.000650","article-title":"Halomonas salicampi sp. nov., a halotolerant and alkalitolerant bacterium isolated from a saltern soil","volume":"65","author":"Lee","year":"2015","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"197","DOI":"10.3389\/FCHEM.2020.00197\/","article-title":"Potashchelins, a suite of lipid Siderophores bearing both L-threo and L-erythro Beta-Hydroxyaspartic acids, acquired from the potash-salt-ore-derived extremophile Halomonas sp","volume":"8","author":"Li","year":"2020","journal-title":"MG34. Front. Chem."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"2037","DOI":"10.1099\/IJS.0.63194-0\/","article-title":"Halomonas koreensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea","volume":"54","author":"Lim","year":"2004","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"2859","DOI":"10.1099\/IJSEM.0.004109\/","article-title":"Halomonas montanilacus sp. Nov., isolated from hypersaline lake pengyanco on the tibetan plateau","volume":"70","author":"Lu","year":"2020","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"937","DOI":"10.1515\/ZNB-1977-0819\/","article-title":"Mikrobielle Produkte, I NMR-Spektren von Nocardamin und Massenspektrum des Tri-O-methyl-nocardamins","volume":"32","author":"Maehr","year":"1977","journal-title":"Z. Naturforsch. B J. Chem. Sci."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"1245","DOI":"10.1126\/science.287.5456.1245","article-title":"Self-assembling amphiphilic siderophores from marine bacteria","volume":"287","author":"Martinez","year":"2000","journal-title":"Science"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"1354","DOI":"10.1002\/JOBM.201600121","article-title":"Transposon-mediated random gene disruption with moderate halophilic bacteria and its application for halophilic bacterial siderophore analysis","volume":"56","author":"Matsui","year":"2016","journal-title":"J. Basic Microbiol."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"D801","DOI":"10.1093\/NAR\/GKAB902","article-title":"TYGS and LPSN: a database tandem for fast and reliable genome-based classification and nomenclature of prokaryotes","volume":"50","author":"Meier-Kolthoff","year":"2022","journal-title":"Nucl. Acids Res."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1007\/BF00173718\/","article-title":"Production of desferrioxamine E and new analogues by directed fermentation and feeding fermentation","volume":"32","author":"Meiwes","year":"1990","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1099\/ijs.0.023150-0","article-title":"Halomonas vilamensis sp. nov., isolated from high-altitude Andean lakes","volume":"61","author":"Menes","year":"2011","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/978-1-4939-3375-4_1\/","article-title":"Structural biology of nonribosomal peptide synthetases","volume":"1401","author":"Miller","year":"2016","journal-title":"Methods Mol. Biol."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/J.BIOS.2018.05.057","article-title":"Siderophore-based biosensors and nanosensors; new approach on the development of diagnostic systems","volume":"117","author":"Nosrati","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.1101\/gr.186072.114","article-title":"CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes","volume":"25","author":"Parks","year":"2015","journal-title":"Genome Res."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"4237","DOI":"10.1016\/S0043-1354(01)00149-X","article-title":"Nitrate reduction with Halomonas campisalis: kinetics of denitrification at pH 9 and 12.5% NaCl","volume":"35","author":"Peyton","year":"2001","journal-title":"Water Res."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1128\/JCM.37.1.199-201.1999","article-title":"Usefulness of Leifson staining method in diagnosis of Helicobacter pylori infection","volume":"37","author":"Piccolomini","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1099\/IJS.0.037036-0\/","article-title":"Halomonas smyrnensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium","volume":"63","author":"Poli","year":"2013","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1139\/CJC-2015-0173\/","article-title":"Vesicle self-assembly of amphiphilic siderophores produced by bacterial isolates from soap Lake, Washington","volume":"94","author":"Serrano Figueroa","year":"2015","journal-title":"Can. J. Chem."},{"key":"ref49","first-page":"607","article-title":"Phenotypic characterization","volume-title":"Methods for general and molecular bacteriology","author":"Smibert","year":"1994"},{"key":"ref50","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1007\/S00203-011-0739-0\/","article-title":"Genomics of iron acquisition in the plant pathogen Erwinia amylovora: insights in the biosynthetic pathway of the siderophore desferrioxamine E","volume":"193","author":"Smits","year":"2011","journal-title":"Arch. Microbiol."},{"key":"ref51","doi-asserted-by":"publisher","first-page":"1880","DOI":"10.1002\/CBDV.201200014","article-title":"Directing the biosynthesis of Putrebactin or Desferrioxamine B in Shewanella putrefaciens through the upstream inhibition of ornithine decarboxylase","volume":"9","author":"Soe","year":"2012","journal-title":"Chem. Biodivers."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"742","DOI":"10.3389\/FMICB.2015.00742","article-title":"NADPH-generating systems in bacteria and archaea","volume":"6","author":"Spaans","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref53","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1021\/IC980635N\/","article-title":"Electrochemical behavior of the Fe(III) complexes of the cyclic hydroxamate siderophores alcaligin and desferrioxamine E","volume":"38","author":"Spasojevi\u0107","year":"1999","journal-title":"Inorg. Chem."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1016\/S1074-5521(99)80082-9","article-title":"The specificity-conferring code of adenylation domains in nonribosomal peptide synthetases","volume":"6","author":"Stachelhaus","year":"1999","journal-title":"Chem. Biol."},{"key":"ref55","doi-asserted-by":"publisher","first-page":"678","DOI":"10.1093\/OXFORDJOURNALS.MOLBEV.A040752","article-title":"Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G+C-content biases","volume":"9","author":"Tamura","year":"1992","journal-title":"Mol. Biol. Evol."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"3022","DOI":"10.1093\/MOLBEV\/MSAB120","article-title":"MEGA11: molecular evolutionary genetics analysis version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"6614","DOI":"10.1093\/nar\/gkw569","article-title":"NCBI prokaryotic genome annotation pipeline","volume":"44","author":"Tatusova","year":"2016","journal-title":"Nucl. Acids Res."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"D603","DOI":"10.1093\/NAR\/","article-title":"MIBiG 3.0: a community-driven effort to annotate experimentally validated biosynthetic gene clusters","volume":"51","author":"Terlouw","year":"2023","journal-title":"Nucl. Acids Res."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1099\/00207713-41-3-377","article-title":"Deleya salina sp. nov., a moderately halophilic gram-negative bacterium","volume":"41","author":"Valderrama","year":"1991","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref60","first-page":"1","article-title":"Halomonas","volume-title":"Bergey\u2019s manual of systematics of Archaea and Bacteria","author":"Ventosa","year":"2021"},{"key":"ref61","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1099\/00207713-30-2-485","article-title":"Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria","volume":"30","author":"Vreeland","year":"1980","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref62","doi-asserted-by":"publisher","first-page":"2855","DOI":"10.1099\/IJS.0.2008\/000729-0","article-title":"Halomonas salifodinae sp. nov., a halophilic bacterium isolated from a salt mine in China","volume":"58","author":"Wang","year":"2008","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref63","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1007\/s10482-014-0189-1","article-title":"Halomonas qiaohouensis sp. nov., isolated from salt mine soil in Southwest China","volume":"106","author":"Wang","year":"2014","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref64","doi-asserted-by":"crossref","DOI":"10.1016\/j.mineng.2021.106774","article-title":"Selective metal extraction by biologically produced siderophores during bioleaching from low-grade primary and secondary mineral resources","author":"Williamson","year":"2021"},{"key":"ref65","doi-asserted-by":"publisher","first-page":"1619","DOI":"10.1099\/IJS.0.65022-0\/","article-title":"Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China","volume":"57","author":"Xu","year":"2007","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref66","doi-asserted-by":"publisher","first-page":"4230","DOI":"10.1099\/IJS.0.051656-0\/","article-title":"Halomonas zincidurans sp. nov., a heavy-metaltolerant bacterium isolated from the deep-sea environment","volume":"63","author":"Xu","year":"2013","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref67","doi-asserted-by":"publisher","first-page":"2899","DOI":"10.1099\/MIC.0.28139-0\/","article-title":"Desferrioxamine E produced by Streptomyces griseus stimulates growth and development of Streptomyces tanashiensis","volume":"151","author":"Yamanaka","year":"2005","journal-title":"Microbiology"},{"key":"ref68","doi-asserted-by":"publisher","first-page":"1171","DOI":"10.1099\/00207713-51-3-1171\/","article-title":"Halomonas marisflavae sp. nov., a halophilic bacterium isolated from the Yellow Sea in Korea","volume":"51","author":"Yoon","year":"2001","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref69","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1007\/S10482-017-0844-4","article-title":"A large-scale evaluation of algorithms to calculate average nucleotide identity","volume":"110","author":"Yoon","year":"2017","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref70","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1099\/00207713-52-1-123\/","article-title":"Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood","volume":"52","author":"Yoon","year":"2002","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref71","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1111\/J.1472-765X.2005.01839.X","article-title":"Characteristics of bacteria showing high denitrification activity in saline wastewater","volume":"42","author":"Yoshie","year":"2006","journal-title":"Lett. Appl. Microbiol."},{"key":"ref72","doi-asserted-by":"publisher","first-page":"1776","DOI":"10.1021\/ACSSYNBIO.9B00106\/","article-title":"Bacterial MbtH-like proteins stimulate nonribosomal peptide Synthetase-derived secondary metabolism in filamentous Fungi","volume":"8","author":"Zwahlen","year":"2019","journal-title":"ACS Synth. Biol."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2023.1194916\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,12]],"date-time":"2023-06-12T04:32:12Z","timestamp":1686544332000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2023.1194916\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,12]]},"references-count":72,"alternative-id":["10.3389\/fmicb.2023.1194916"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2023.1194916","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,12]]},"article-number":"1194916"}}