{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T20:27:06Z","timestamp":1791923226013,"version":"4.3.4"},"reference-count":57,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T00:00:00Z","timestamp":1695168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Coumarin-3-carboxylic acid (3-CCA), previously screened from natural coumarins, was found to possess strong antibacterial activity against\n                    <jats:italic>Acidovorax citrulli<\/jats:italic>\n                    (\n                    <jats:italic>Ac<\/jats:italic>\n                    ). In order to further evaluate the activity of this compound against plant bacterial pathogens and explore its potential value as a bactericidal lead compound, the activity of 3-CCA against 14 plant pathogenic bacteria\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    was tested. Results showed that 3-CCA exhibited strong\n                    <jats:italic>in vitro<\/jats:italic>\n                    activities against\n                    <jats:italic>Ac, Ralstonia solanacearum, Xanthomonas axonopodis<\/jats:italic>\n                    pv.\n                    <jats:italic>manihotis, X. oryzae<\/jats:italic>\n                    pv.\n                    <jats:italic>oryzae<\/jats:italic>\n                    , and\n                    <jats:italic>Dickeya zeae<\/jats:italic>\n                    with EC\n                    <jats:sub>50<\/jats:sub>\n                    values ranging from 26.64 \u03bcg\/mL to 40.73 \u03bcg\/mL.\n                    <jats:italic>Pot experiment results<\/jats:italic>\n                    showed that 3-CCA had powerful protective and curative effects against\n                    <jats:italic>Ac<\/jats:italic>\n                    . In addition, the protective efficiency of 3-CCA was almost equivalent to that of thiodiazole copper at the same concentration. The results of SEM and TEM observation and conductivity tests showed that 3-CCA disrupted the integrity of the cell membrane and inhibited polar flagella growth. Furthermore, 3-CCA resulted in reductions in motility and extracellular exopolysaccharide (EPS) production of\n                    <jats:italic>Ac<\/jats:italic>\n                    while inhibiting the biofilm formation of\n                    <jats:italic>Ac<\/jats:italic>\n                    . These findings indicate that 3-CCA could be a promising natural lead compound against plant bacterial pathogens to explore novel antibacterial agents.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2023.1207125","type":"journal-article","created":{"date-parts":[[2023,9,21]],"date-time":"2023-09-21T03:27:09Z","timestamp":1695266829000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Antibacterial activities of coumarin-3-carboxylic acid against Acidovorax citrulli"],"prefix":"10.3389","volume":"14","author":[{"given":"Fa-Di","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huo-Chun","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-Xin","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu-Shuang","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Xia","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,9,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"24","DOI":"10.5530\/srp.2017.1.6","article-title":"Antioxidant activity of coumarins","volume":"8","author":"Al-Majedy","year":"2016","journal-title":"Syst. 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