{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T21:15:37Z","timestamp":1791580537012,"version":"4.3.4"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T00:00:00Z","timestamp":1691020800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Antibiotic-resistant microbes pose one of the biggest challenges of the current century. While areas with proximity to human impact are closely studied, a lot is yet to learn about antimicrobial resistance in remote regions like the cryosphere. Nowadays, antibiotic (AB) resistance is considered a pollution that has reached the Earth\u2019s most pristine areas. However, monitoring of resistant environmental bacteria therein faces several challenges that inhibit scientific progress in this field. Due to many cultivation-based antibiotic susceptibility tests being optimized for mesophilic pathogenic microorganisms, many researchers opt for expensive molecular biological approaches to detect antibiotic resistance in the cryosphere. However, some disadvantages of these methods prohibit effective comprehensive monitoring of resistant bacteria in pristine areas, hence we suggest established cultivation-based approaches when looking for antimicrobial resistance in the cryosphere. In this study, we compared two common antibiotic susceptibility tests and optimized them to meet the needs of psychrophilic microorganisms. The resulting cultures thereof originated from cryospheric habitats with differing anthropogenic impacts. The results show that these methods are applicable to detect antibiotic resistance in cryospheric habitats and could potentially increase the comparability between studies.<\/jats:p>","DOI":"10.3389\/fmicb.2023.1212378","type":"journal-article","created":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T10:41:28Z","timestamp":1691059288000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["A comparative approach to confirm antibiotic-resistant microbes in the cryosphere"],"prefix":"10.3389","volume":"14","author":[{"given":"Daniel","family":"Gattinger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Pichler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Weil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Sattler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,8,3]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1038\/nrmicro2312","article-title":"Call of the wild: antibiotic resistance genes in natural environments","volume":"8","author":"Allen","year":"2010","journal-title":"Nat Rev Microbiol"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"144","DOI":"10.4161\/viru.2.2.15014","article-title":"The intrinsic resistome of Pseudomonas aeruginosa to \u03b2-lactams","volume":"2","author":"Alvarez-Ortega","year":"2011","journal-title":"Virulence"},{"key":"ref3","first-page":"574","article-title":"Single disc versus multiple disc and plate dilution techniques for antibiotic sensitivity testing","volume":"7","author":"Bauer","year":"1959","journal-title":"Antibiot. Annu."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1038\/nrmicro3439","article-title":"Tackling antibiotic resistance: the environmental framework","volume":"13","author":"Berendonk","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"evab028","DOI":"10.1093\/gbe\/evab028","article-title":"sangeranalyseR: simple and interactive processing of sanger sequencing data in R","volume":"13","author":"Chao","year":"2021","journal-title":"Genome Biol. Evol."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/S0065-7743(08)60537-0","article-title":"Transport of antibiotics in bacteria","volume":"24","author":"Chopra","year":"1989","journal-title":"Annu. Rep. Med. Chem."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"237","DOI":"10.3390\/antibiotics2020237","article-title":"Phenotypic resistance to antibiotics","volume":"2","author":"Corona","year":"2013","journal-title":"Antibiotics"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1038\/nature10388","article-title":"Antibiotic resistance is ancient","volume":"477","author":"D'Costa","year":"2011","journal-title":"Nature"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1126\/science.1120800","article-title":"Sampling the antibiotic Resistome","volume":"311","author":"D'Costa","year":"2006","journal-title":"Science"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"508","DOI":"10.1002\/embr.201338170","article-title":"Some like it cold: understanding the survival strategies of psychrophiles","volume":"15","author":"de Maayer","year":"2014","journal-title":"EMBO Rep."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"808","DOI":"10.1016\/j.bbapap.2008.11.005","article-title":"Outer membrane permeability and antibiotic resistance","volume":"1794","author":"Delcour","year":"2009","journal-title":"Biochim. 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Microbiol."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"e5629","DOI":"10.1371\/journal.pone.0005629","article-title":"Genetic architecture of intrinsic antibiotic susceptibility","volume":"4","author":"Girgis","year":"2009","journal-title":"PLoS One"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"890","DOI":"10.1038\/s41467-019-08719-8","article-title":"Extreme slow growth as alternative strategy to survive deep starvation in bacteria","volume":"10","author":"Gray","year":"2019","journal-title":"Nat. Commun."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1128\/jcm.19.2.279-280.1984","article-title":"Five-hour Novobiocin test for differentiation of coagulase-negative staphylococci","volume":"19","author":"Harrington","year":"1984","journal-title":"J. Clin. Microbiol."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1080\/15287390600975137","article-title":"Potential ecological and human health impacts of antibiotics and antibiotic-resistant bacteria from wastewater treatment plants","volume":"10","author":"Kim","year":"2007","journal-title":"J. Toxicol. Environ. Health B Crit. Rev."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/j.envint.2019.01.034","article-title":"Understanding drivers of antibiotic resistance genes in high Arctic soil ecosystems","volume":"125","author":"McCann","year":"2019","journal-title":"Environ. Int."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"997","DOI":"10.2134\/jeq2017.12.0477","article-title":"Sources of antibiotic resistance genes in a Rural River system","volume":"47","author":"McConnell","year":"2018","journal-title":"J. Environ. Qual."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1128\/br.39.2.144-167.1975","article-title":"Psychrophilic bacteria","volume":"39","author":"Morita","year":"1975","journal-title":"Bacteriol. Rev."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"1541","DOI":"10.1111\/jam.14063","article-title":"Background levels of microorganisms in the busy urban environment of transport hubs","volume":"125","author":"Patel","year":"2018","journal-title":"J. Appl. Microbiol."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1086\/511439","article-title":"Germs on a plane: aircraft, international travel, and the global spread of disease","volume":"195","author":"Pavia","year":"2007","journal-title":"J. Infect. Dis."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"13803","DOI":"10.1038\/ncomms13803","article-title":"A diverse intrinsic antibiotic resistome from a cave bacterium","volume":"7","author":"Pawlowski","year":"2016","journal-title":"Nat. Commun."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"1178474","DOI":"10.3389\/fmicb.2023.1178474","article-title":"Discovery of plastic-degrading microbial strains isolated from the alpine and Arctic terrestrial plastisphere","volume":"14","author":"R\u00fcthi","year":"2023","journal-title":"Front. Microbiol."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1292\/jvms1922.17.3_205","article-title":"On the gram-differentiation of bacteria by the simplest method","volume":"17","author":"Ryu","year":"1938","journal-title":"J. Japanese Soc. 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Fish Dis."},{"key":"ref33","volume-title":"Breakpoint tables for interpretation of MICs and zone diameters","year":""},{"key":"ref34","volume-title":"EUCAST disk diffusion test methodology","year":""},{"key":"ref35","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1111\/zph.12017","article-title":"Antimicrobial use and resistance in aquaculture: findings of a globally administered survey of aquaculture-allied professionals","volume":"60","author":"Tu\u0161evljak","year":"2012","journal-title":"Zoonoses Public Health"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"43","DOI":"10.2323\/jgam.56.43","article-title":"Application of real-time PCR array to the multiple detection of antibiotic resistant genes in glacier ice samples","volume":"56","author":"Ushida","year":"2010","journal-title":"J. Gen. Appl. Microbiol."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"e65567","DOI":"10.1371\/journal.pone.0065567.g001","article-title":"The culturable soil antibiotic resistome: a community of multi-drug resistant bacteria","volume":"8","author":"Walsh","year":"2013","journal-title":"PLoS One"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1186\/s40168-017-0249-7","article-title":"Legal immigrants: invasion of alien microbial communities during winter occurring desert dust storms","volume":"5","author":"Weil","year":"2017","journal-title":"Microbiome"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"3515","DOI":"10.1128\/AAC.00012-09","article-title":"Protein kinase G is required for intrinsic antibiotic resistance in mycobacteria","volume":"53","author":"Wolff","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1007\/s00253-008-1829-z","article-title":"Antibiotic resistance genes in water environment","volume":"82","author":"Zhang","year":"2009","journal-title":"Appl. Microbiol. 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