{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T05:15:15Z","timestamp":1793078115281,"version":"4.5.0"},"reference-count":68,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T00:00:00Z","timestamp":1691366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Antibiotic resistance in bacteria has emerged as a serious public health threat worldwide. Aquatic environments including irrigation-purpose wastewaters facilitate the emergence and transmission of antibiotic-resistant bacteria and antibiotic resistance genes leading to detrimental effects on human health and environment sustainability. Considering the paramount threat of ever-increasing antibiotic resistance to human health, there is an urgent need for continuous environmental monitoring of antibiotic-resistant bacteria and antibiotic resistance genes in wastewater being used for irrigation in Indian agro-ecosystems. In this study, the prevalence of antibiotic resistance in Gram-negative bacteria isolated from irrigation-purpose wastewater samples from Sirmaur and Solan districts of Himachal Pradesh was determined. Bacterial isolates of genera <jats:italic>Escherichia, Enterobacter, Hafnia, Shigella, Citrobacter<\/jats:italic>, and <jats:italic>Klebsiella<\/jats:italic> obtained from 11 different geographical locations were found to exhibit resistance against ampicillin, amoxyclav, cefotaxime, co-trimoxazole, tobramycin, cefpodoxime and ceftazidime. However, all the isolates were sensitive to aminoglycoside antibiotic gentamicin. <jats:italic>Enterobacter<\/jats:italic> spp. and <jats:italic>Escherichia coli<\/jats:italic> showed predominance among all the isolates. Multidrug-resistance phenotype was observed with isolate AUK-06 (<jats:italic>Enterobacter<\/jats:italic> sp.) which exhibited resistant to five antibiotics. Isolate AUK-02 and AUK-09, both <jats:italic>E. coli<\/jats:italic> strains showed resistant phenotypes to four antibiotics each. Phenotypic detection revealed that six isolates were positive for extended-spectrum \u03b2-lactamases which includes two isolates from <jats:italic>Enterobacter<\/jats:italic> spp. and <jats:italic>E. coli<\/jats:italic> each and one each from <jats:italic>Shigella<\/jats:italic> sp. and <jats:italic>Citrobacter<\/jats:italic> sp. Overall, the findings revealed the occurrence of antibiotic resistant and ESBL-positive bacterial isolates in wastewaters utilized for irrigation purpose in the study area and necessitate continuous monitoring and precautionary interventions. The outcomes of the study would be of significant clinical, epidemiological, and agro-environmental importance in designing effective wastewater management and environmental pollution control strategies.<\/jats:p>","DOI":"10.3389\/fmicb.2023.1227132","type":"journal-article","created":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T13:20:12Z","timestamp":1691414412000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Prevalence of antibiotic-resistant Gram-negative bacteria having extended-spectrum \u03b2-lactamase phenotypes in polluted irrigation-purpose wastewaters from Indian agro-ecosystems"],"prefix":"10.3389","volume":"14","author":[{"given":"Achhada Ujalkaur","family":"Avatsingh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilpa","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilippreet","family":"Kour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukta","family":"Arora","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheetal","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divya","family":"Joshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prem Prashant","family":"Chaudhary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kahkashan","family":"Perveen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohab Amin","family":"Kamal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasib","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,8,7]]},"reference":[{"key":"ref1","volume-title":"Minimum quality requirements for water reuse in agricultural irrigation and aquifer recharge-towards a legal instrument on water reuse at EU level, EUR 28962 EN","author":"Alcalde-Sanz","year":"2017"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"592","DOI":"10.2166\/wh.2021.085","article-title":"Lentic and effluent water of Delhi-NCR: a reservoir of multidrug-resistant bacteria harbouring blaCTX-M, blaTEM and blaSHV type ESBL genes","volume":"19","author":"Ali","year":"2021","journal-title":"J. Water Health"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1080\/10934529.2015.964602","article-title":"Antibiotic resistance in wastewater: occurrence and fate of Enterobacteriaceae producers of class a and class C \u03b2-lactamases. J. Environ. Sci. Health","volume":"50","author":"Amador","year":"2015","journal-title":"A Tox. Hazard. Subst. Environ. Eng."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"e0186922","DOI":"10.1128\/aem.01869-22","article-title":"Characterization of Escherichia coli and other Enterobacterales resistant to extended-spectrum cephalosporins isolated from dairy manure in Ontario Canada","volume":"89","author":"Anderson","year":"2023","journal-title":"Appl. Environ. Microbiol."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis","volume":"399","year":"2022","journal-title":"Lancet"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1016\/j.scitotenv.2014.09.084","article-title":"Distribution and molecular characterization of genes encoding CTX-M and AmpC \u03b2-lactamases in Escherichia coli isolated from an Indian urban aquatic environment","volume":"505","author":"Bajaj","year":"2015","journal-title":"Sci. Total Environ."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1016\/j.ijfoodmicro.2015.02.023","article-title":"Detection of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in vegetables, soil and water of the farm environment in Tunisia","volume":"203","author":"Ben Said","year":"2015","journal-title":"Int. J. Food Microbiol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41545-020-00087-x","article-title":"Proclivities for prevalence and treatment of antibiotics in the ambient water: a review","volume":"3","author":"Bhagat","year":"2020","journal-title":"npj Clean Water"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1080\/14787210.2023.2194633","article-title":"Classification for \u03b2-lactamases: historical perspectives","volume":"21","author":"Bush","year":"2023","journal-title":"Expert Rev. Anti-Infect. Ther."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"e00047","DOI":"10.1128\/CMR.00047-19","article-title":"Epidemiology of \u03b2-lactamase-producing pathogens","volume":"33","author":"Bush","year":"2020","journal-title":"Clin. Microbiol. Rev."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"466","DOI":"10.1016\/j.mib.2006.08.011","article-title":"The CTX-M \u03b2-lactamase pandemic","volume":"9","author":"Canton","year":"2006","journal-title":"Curr. Opin. Microbiol."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"dlab092","DOI":"10.1093\/jacamr\/dlab092","article-title":"Extended-spectrum \u03b2-lactamases: an update on their characteristics, epidemiology and detection","volume":"3","author":"Castanheira","year":"2021","journal-title":"JAC Antimicrob. Resist."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"115502","DOI":"10.1016\/j.envpol.2020.115502","article-title":"Co-occurrence of multidrug resistance, \u03b2-lactamase and plasmid mediated AmpC genes in bacteria isolated from river ganga, northern India","volume":"267","author":"Chaturvedi","year":"2020","journal-title":"Environ. Pollut."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"129693","DOI":"10.1016\/j.chemosphere.2021.129693","article-title":"Dissemination of antibiotic resistance genes, mobile genetic elements, and efflux genes in anthropogenically impacted riverine environments","volume":"273","author":"Chaturvedi","year":"2021","journal-title":"Chemosphere"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"lxad034","DOI":"10.1093\/jambio\/lxad034","article-title":"Freshwater environment as a reservoir of extended-spectrum \u03b2-lactamase-producing Enterobacteriaceae","volume":"134","author":"Cho","year":"2023","journal-title":"J. Appl. Microbiol."},{"key":"ref16","volume-title":"Clinical and laboratory standards institute. M100 performance standards for antimicrobial susceptibility testing","year":"2018"},{"key":"ref17","volume-title":"Clinical and laboratory standards institute. M100 performance standards for antimicrobial susceptibility testing","year":"2020"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"62","DOI":"10.3390\/antibiotics12010062","article-title":"KPC-producing Enterobacterales from Douro River, Portugal-persistent environmental contamination by putative healthcare settings","volume":"12","author":"Dantas Palmeira","year":"2022","journal-title":"Antibiotics"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1111\/j.1469-0691.2007.01846.x","article-title":"Phenotypic detection of extended-spectrum beta-lactamase production in Enterobacteriaceae: review and bench guide","volume":"14","author":"Drieux","year":"2008","journal-title":"Clin. Microbiol. Infect."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1007\/s13593-011-0062-9","article-title":"Occurrence, fate, and ecotoxicity of antibiotics in agro-ecosystems","volume":"32","author":"Du","year":"2012","journal-title":"A review. Agron. Sustain. Dev."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"e0204805","DOI":"10.1371\/journal.pone.0204805","article-title":"Community level antibiotic utilization in India and its comparison Vis-\u00e0-Vis European countries: evidence from pharmaceutical sales data","volume":"13","author":"Farooqui","year":"2018","journal-title":"PLoS One"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"dlac112","DOI":"10.1093\/jacamr\/dlac112","article-title":"Antibiotic consumption in India: geographical variations and temporal changes between 2011 and 2019","volume":"4","author":"Fazaludeen Koya","year":"2022","journal-title":"JAC Antimicrob. Resist."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"1112876","DOI":"10.3389\/fceng.2022.1112876","article-title":"Not a waste: wastewater surveillance to enhance public health","volume":"4","author":"Gitter","year":"2023","journal-title":"Front. Chem. Eng."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1002\/etc.5289","article-title":"Antimicrobial resistance in rivers: a review of the genes detected and new challenges","volume":"41","author":"Grenni","year":"2022","journal-title":"Environ. Toxicol. Chem."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"7706","DOI":"10.3390\/ijerph17217706","article-title":"Monitoring of water quality, antibiotic residues, and antibiotic-resistant Escherichia coli in the Kshipra River in India over a 3-year period","volume":"17","author":"Hanna","year":"2020","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/s12302-019-0283-0","article-title":"Regulating water reuse for agricultural irrigation: risks related to organic micro-contaminants","volume":"32","author":"Helmecke","year":"2020","journal-title":"Environ. Sci. Eur."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.mib.2019.10.008","article-title":"Antibiotics: past, present and future","volume":"51","author":"Hutchings","year":"2019","journal-title":"Curr. Opin. Microbiol."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"e0282835","DOI":"10.1371\/journal.pone.0282835","article-title":"Multidrug-resistant extended spectrum \u03b2-lactamase (ESBL)-producing Escherichia coli from farm produce and agricultural environments in Edo state","volume":"18","author":"Igbinosa","year":"2023","journal-title":"Nigeria. PLoS ONE"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"e00174","DOI":"10.1128\/CMR.00174-20","article-title":"The changing face of the family Enterobacteriaceae (order: \"Enterobacterales\"): new members, taxonomic issues, geographic expansion, and new diseases and disease syndromes","volume":"34","author":"Janda","year":"2021","journal-title":"Clin. Microbiol. Rev."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1007\/s44197-021-00008-2","article-title":"Easy access to antibiotics; spread of antimicrobial resistance and implementation of one health approach in India","volume":"11","author":"Jani","year":"2021","journal-title":"J. Epidemol. Glob. Health."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1016\/j.tim.2017.09.005","article-title":"Antibiotic-resistance genes in wastewater","volume":"26","author":"Karkman","year":"2018","journal-title":"Trends Microbiol."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1128\/aem.46.1.165-170.1983","article-title":"Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods","volume":"46","author":"Krumperman","year":"1983","journal-title":"Appl. Environ. Microbiol."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/j.chemosphere.2017.08.133","article-title":"Sewage treatment effluents in Delhi: a key contributor of \u03b2-lactam resistant bacteria and genes to the environment","volume":"188","author":"Lamba","year":"2017","journal-title":"Chemosphere"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1038\/s41579-021-00649-x","article-title":"Antibiotic resistance in the environment","volume":"20","author":"Larsson","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.1007\/s11157-021-09588-8","article-title":"Antibiotic resistance dissemination in wastewater treatment plants: a challenge for the reuse of treated wastewater in agriculture","volume":"20","author":"Leiva","year":"2021","journal-title":"Rev. Environ. Sci. Biotechnol."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"9149","DOI":"10.1021\/acs.est.1c08918","article-title":"Antimicrobial resistance monitoring of water environments: a framework for standardized methods and quality control","volume":"56","author":"Liguori","year":"2022","journal-title":"Environ. Sci. Technol."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1111\/j.1469-0691.2007.01857.x","article-title":"Defining an extended-spectrum beta-lactamase","volume":"14","author":"Livermore","year":"2008","journal-title":"Clin. Microbiol. Infect."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"816943","DOI":"10.3389\/fpubh.2022.816943","article-title":"System mapping of antimicrobial resistance to combat a rising Global Health crisis","volume":"10","author":"Matthiessen","year":"2022","journal-title":"Front. Public Health"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"152001","DOI":"10.1016\/j.scitotenv.2021.152001","article-title":"Wastewater irrigation in India: current status, impacts and response options","volume":"808","author":"Minhas","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"709418","DOI":"10.3389\/fmicb.2021.709418","article-title":"Extended-spectrum beta-lactamase producing-Escherichia coli isolated from irrigation waters and produce in Ecuador","volume":"12","author":"Montero","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"508","DOI":"10.3892\/etm.2022.11435","article-title":"Phenotypic and genotypic detection methods for antimicrobial resistance in ESKAPE pathogens (review)","volume":"24","author":"Muntean","year":"2022","journal-title":"Exp. Ther. Med."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1007\/s11274-022-03334-0","article-title":"Occurrence of antibiotics and bacterial resistance genes in wastewater: resistance mechanisms and antimicrobial resistance control approaches","volume":"38","author":"Mutuku","year":"2022","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"1140","DOI":"10.3390\/antibiotics10091140","article-title":"Detection of multidrug-resistant Enterobacterales-from ESBLs to Carbapenemases","volume":"10","author":"Noster","year":"2021","journal-title":"Antibiotics (Basel)."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"6781","DOI":"10.1038\/s41598-021-86378-w","article-title":"Evaluation of 16S rRNA primer sets for characterisation of microbiota in paediatric patients with autism spectrum disorder","volume":"11","author":"Palkova","year":"2021","journal-title":"Sci. Rep."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"e00088","DOI":"10.1128\/CMR.00088-17","article-title":"Mobile genetic elements associated with antimicrobial resistance","volume":"31","author":"Partridge","year":"2018","journal-title":"Clin. Microbiol. Rev."},{"key":"ref9001","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1093\/oxfordjournals.molbev.a040454","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref48","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-023-00902-5","article-title":"Translating eco-evolutionary biology into therapy to tackle antibiotic resistance","author":"Sanz-Garc\u00eda","year":"2023","journal-title":"Nat. Rev. Microbiol."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1186\/s40560-020-0429-6","article-title":"Molecular diversity of extended-spectrum \u03b2-lactamases and carbapenemases, and antimicrobial resistance","volume":"8","author":"Sawa","year":"2020","journal-title":"J. Intensive Care"},{"key":"ref50","doi-asserted-by":"publisher","first-page":"147269","DOI":"10.1016\/j.scitotenv.2021.147269","article-title":"Prevalence of multidrug-resistant and extended-spectrum beta-lactamase-producing Escherichia coli in urban community wastewater","volume":"785","author":"Schmiege","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref51","doi-asserted-by":"publisher","first-page":"277","DOI":"10.4014\/jmb.2210.10044","article-title":"Resistome study in aquatic environments","volume":"33","author":"Shin","year":"2023","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref53","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s42452-021-04596-3","article-title":"Identification and characterization of colistin-resistant E. coli and K. pneumoniae isolated from lower Himalayan region of India","volume":"3","author":"Singh","year":"2021","journal-title":"SN Appl. Sci."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"622891","DOI":"10.3389\/fmicb.2021.622891","article-title":"Antibiotic resistance profiles and molecular characteristics of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae isolated from shrimp aquaculture farms in Kerala","volume":"12","author":"Sivaraman","year":"2021","journal-title":"India. Front. Microbiol."},{"key":"ref55","doi-asserted-by":"publisher","first-page":"90","DOI":"10.3390\/antibiotics12010090","article-title":"A novel single-tube eicosaplex\/octaplex PCR system for the detection of extended-spectrum \u03b2-lactamases, plasmid-mediated AmpC \u03b2-lactamases, and integrons in gram-negative bacteria","volume":"12","author":"Soliman","year":"2023","journal-title":"Antibiotics (Basel)."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"2091","DOI":"10.1038\/s41598-021-81315-3","article-title":"Detection and characterization of ESBL-producing Enterobacteriaceae from the gut of subsistence farmers, their livestock, and the surrounding environment in rural Nepal","volume":"11","author":"Subramanya","year":"2021","journal-title":"Sci. Rep."},{"key":"ref9002","doi-asserted-by":"publisher","first-page":"1596","DOI":"10.1093\/molbev\/msm092","article-title":"MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.","volume":"24","author":"Tamura","year":"2007","journal-title":"Mol. Biol. Evol."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"ovac044","DOI":"10.1093\/lambio\/ovac044","article-title":"Characterisation of ESBL\/AmpC-producing Enterobacteriaceae isolated from poultry farms in peninsular Malaysia","volume":"76","author":"Tan","year":"2023","journal-title":"Lett. Appl. Microbiol."},{"key":"ref9003","doi-asserted-by":"publisher","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"22","author":"Thompson","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1016\/j.jgar.2023.04.015","article-title":"Phenotypic and genotypic characteristics of beta-lactamase dominant with CARBA, AmpC, and ESBL-producing bacteria in municipal wastewater influent in Helsinki","volume":"33","author":"Tiwari","year":"2023","journal-title":"Finland. J. Glob. Antimicrob. Resist."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"883282","DOI":"10.3389\/frwa.2022.883282","article-title":"Wastewater treatment works: a last line of defense for preventing antibiotic resistance entry into the environment","volume":"4","author":"Tucker","year":"2022","journal-title":"Front. Water"},{"key":"ref60","doi-asserted-by":"publisher","first-page":"717809","DOI":"10.3389\/fmicb.2021.717809","article-title":"A review on occurrence and spread of antibiotic resistance in wastewaters and in wastewater treatment plants: mechanisms and perspectives","volume":"12","author":"Uluseker","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref61","doi-asserted-by":"publisher","first-page":"e0269421","DOI":"10.1128\/spectrum.02694-21","article-title":"Multidrug-resistant gram-negative bacteria and extended-spectrum \u03b2-lactamase-producing Klebsiella pneumoniae from the poultry farm environment","volume":"10","author":"Veloo","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref62","doi-asserted-by":"publisher","first-page":"173","DOI":"10.3389\/fmicb.2016.00173","article-title":"Dissemination of antimicrobial resistance in microbial ecosystems through horizontal gene transfer","volume":"7","author":"von Wintersdorff","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref63","doi-asserted-by":"publisher","first-page":"e56033","DOI":"10.15252\/embr.202256033","article-title":"Fighting antibiotic resistance-strategies and (pre)clinical developments to find new antibacterials","volume":"24","author":"Walesch","year":"2023","journal-title":"EMBO Rep."},{"key":"ref64","doi-asserted-by":"publisher","first-page":"140997","DOI":"10.1016\/j.scitotenv.2020.140997","article-title":"Occurrence and fate of antibiotics, antibiotic resistant genes (ARGs) and antibiotic resistant bacteria (ARB) in municipal wastewater treatment plant: an overview","volume":"744","author":"Wang","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref65","year":"2023"},{"key":"ref66","doi-asserted-by":"publisher","first-page":"705","DOI":"10.2166\/wh.2021.112","article-title":"Prevalence of extended-spectrum \u03b2-lactamase (ESBL)-producing Enterobacteriaceae in wastewater: a systematic review and meta-analysis","volume":"19","author":"Zaatout","year":"2021","journal-title":"J. Water Health"},{"key":"ref67","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1007\/s10661-020-08319-w","article-title":"Gram-negative bacteria carrying \u03b2-lactamase encoding genes in hospital and urban wastewater in Brazil","volume":"192","author":"Zagui","year":"2020","journal-title":"Enviro. Monit. 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