{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:07:26Z","timestamp":1753891646247,"version":"3.41.2"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T00:00:00Z","timestamp":1698969600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013928","name":"Millennium Institute on Immunology and Immunotherapy","doi-asserted-by":"publisher","award":["ICN09_016\/ICN 2021_045","P09\/016-F"],"award-info":[{"award-number":["ICN09_016\/ICN 2021_045","P09\/016-F"]}],"id":[{"id":"10.13039\/501100013928","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Excisable genomic islands (EGIs) are horizontally acquired genetic elements that harbor an array of genes with diverse functions. ROD21 is an EGI found integrated in the chromosome of <jats:italic>Salmonella enterica<\/jats:italic> serovar Enteritidis (<jats:italic>Salmonella<\/jats:italic> ser. Enteritidis). While this island is known to be involved in the capacity of <jats:italic>Salmonella<\/jats:italic> ser. Enteritidis to cross the epithelial barrier and colonize sterile organs, the role of most ROD21 genes remains unknown, and thus, the identification of their function is fundamental to understanding the impact of this EGI on bacterium pathogenicity. Therefore, in this study, we used a bioinformatical approach to evaluate the function of ROD21-encoded genes and delve into the characterization of <jats:italic>SEN1990<\/jats:italic>, a gene encoding a putative DNA-binding protein. We characterized the predicted structure of SEN1990, finding that this protein contains a three-stranded winged helix-turn-helix (wHTH) DNA-binding domain. Additionally, we identified homologs of SEN1990 among other members of the EARL EGIs. Furthermore, we deleted <jats:italic>SEN1990<\/jats:italic> in <jats:italic>Salmonella<\/jats:italic> ser. Enteritidis, finding no differences in the replication or maintenance of the excised ROD21, contrary to what the previous Refseq annotation of the protein suggests. High-throughput RNA sequencing was carried out to evaluate the effect of the absence of <jats:italic>SEN1990<\/jats:italic> on the bacterium\u2019s global transcription. We found a downregulated expression of <jats:italic>oafB<\/jats:italic>, an SPI-17-encoded acetyltransferase involved in O-antigen modification, which was restored when the deletion mutant was complemented ectopically. Additionally, we found that strains lacking <jats:italic>SEN1990<\/jats:italic> had a reduced capacity to colonize sterile organs in mice. Our findings suggest that <jats:italic>SEN1990<\/jats:italic> encodes a wHTH domain-containing protein that modulates the transcription of <jats:italic>oafB<\/jats:italic> from the SPI-17, implying a crosstalk between these pathogenicity islands and a possible new role of ROD21 in the pathogenesis of <jats:italic>Salmonella<\/jats:italic> ser. Enteritidis.<\/jats:p>","DOI":"10.3389\/fmicb.2023.1236458","type":"journal-article","created":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T10:19:07Z","timestamp":1699006747000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["SEN1990 is a predicted winged helix-turn-helix protein involved in the pathogenicity of Salmonella enterica serovar Enteritidis and the expression of the gene oafB in the SPI-17"],"prefix":"10.3389","volume":"14","author":[{"given":"Guillermo","family":"Hoppe-Elsholz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Pi\u00f1a-Iturbe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omar P.","family":"Vallejos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isidora D.","family":"Suazo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javiera","family":"Sep\u00falveda-Alfaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Pereira-S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohana","family":"Mart\u00ednez-Balboa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo A.","family":"Catal\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Reyes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Scaff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franco","family":"Bassi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia","family":"Campos-Gajardo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Avil\u00e9s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos A.","family":"Santiviago","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexis M.","family":"Kalergis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan M.","family":"Bueno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2023,11,3]]},"reference":[{"key":"ref9001","doi-asserted-by":"publisher","first-page":"710","DOI":"10.1038\/90429","volume":"8","author":"Alekshun","year":"2001","journal-title":"The crystal structure of MarR, a regulator of multiple antibiotic resistance, at 2.3 \u00c5 resolution Nature Structural Biology"},{"key":"ref1","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/j.femsre.2004.12.008","article-title":"The many faces of the helix-turn-helix domain: transcription regulation and beyond","volume":"29","author":"Aravind","year":"2005","journal-title":"FEMS Microbiol. 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