{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:29:59Z","timestamp":1793039399971,"version":"4.5.0"},"reference-count":54,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:00:00Z","timestamp":1695686400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42276110"],"award-info":[{"award-number":["42276110"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Zoonotic parasites pose significant health risks globally. In the present study, we combined a microfluidic chip with loop-mediated isothermal amplification (on-chip LAMP) to detect five zoonotic parasites:\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    ,\n                    <jats:italic>Cryptosporidium parvum<\/jats:italic>\n                    ,\n                    <jats:italic>Cryptosporidium hominis<\/jats:italic>\n                    ,\n                    <jats:italic>Clonorchis sinensis<\/jats:italic>\n                    , and\n                    <jats:italic>Taenia solium<\/jats:italic>\n                    . This method enabled the simultaneous parallel analysis of five genetic markers from a maximum of four samples per chip. The on-chip LAMP assay was conducted in a highly automated format\n                    <jats:italic>via<\/jats:italic>\n                    the addition (by pipetting) of each sample in a single operation. The reaction was performed in volumes as low as 5\u2009\u03bcL at a temperature of 65\u00b0C for 60\u2009min, achieving limits of detection ranging from 10\n                    <jats:sup>\u22122<\/jats:sup>\n                    to 10\n                    <jats:sup>\u22123<\/jats:sup>\n                    \u2009pg.\/\u03bcL of recombinant plasmid DNA. All the time-to-positive values were less than 40\u2009min, and almost all the coefficients of variation were less than 10%, even when using limit of detection concentrations for multiple pathogens, indicating robust reproducibility among replicates. The clinical sensitivity and specificity for detecting 135 field samples were 98.08 and 97.59%, respectively, compared with traditional biological methods, indicating good applicability in the detection of field samples. This on-chip LAMP assay allows for low reagent consumption, ease of operation, and multiple analyses of samples and genetic targets, and is applicable for on-site detection and the routine monitoring of multiple zoonotic parasites.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2023.1238376","type":"journal-article","created":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T00:41:06Z","timestamp":1695775266000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Parallel detection of multiple zoonotic parasites using a real-time fluorogenic loop-mediated isothermal amplification-based quadruple-sample microfluidic chip"],"prefix":"10.3389","volume":"14","author":[{"given":"Yu-Xin","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Rong","family":"Lou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Jun","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian-Jin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong-Jie","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang-Jie","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Xian","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gui-Zong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ai-Fang","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,9,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1016\/j.ijheh.2018.04.006","article-title":"Comparison of current methods used to detect Cryptosporidium oocysts in stools","volume":"221","author":"Ahmed","year":"2018","journal-title":"Int. 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