{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:18:43Z","timestamp":1787026723530,"version":"build-2736575974"},"reference-count":65,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T00:00:00Z","timestamp":1694649600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    <jats:italic>Brucella abortus<\/jats:italic>\n                    is a facultative extracellular-intracellular bacterial zoonotic pathogen worldwide. It is also a major cause of abortion in bovines, generating economic losses. The two-component regulatory system BvrR\/BvrS modulates the expression of genes required to transition from extracellular to intracellular lifestyles. However, few regulatory regions of BvrR direct target genes have been studied. In this study, we characterized the regulatory region of\n                    <jats:italic>omp25<\/jats:italic>\n                    , a gene encoding an outer membrane protein that is positively regulated by TCS BvrR\/BvrS. By\n                    <jats:italic>omp25<\/jats:italic>\n                    -\n                    <jats:italic>lacZ<\/jats:italic>\n                    reporter fusions and \u03b2-galactosidase activity assays, we found that the region between-262 and\u2009+\u2009127 is necessary for transcriptional activity, particularly a 111-bp long fragment located from-262 to \u2212152. In addition, we demonstrated the binding of P-BvrR to three sites within the \u2212140 to +1 region. Two of these sites were delimited between \u221218 to +1 and\u2009\u2212\u200999 to \u221276 by DNase I footprinting and called DNA regulatory boxes 1 and 2, respectively. The third binding site (box 3) was delimited from \u2212140 to \u2212122 by combining EMSA and fluorescence anisotropy results. A molecular docking analysis with HDOCK predicted BvrR-DNA interactions between 11, 13, and 12 amino acid residue-nucleotide pairs in boxes 1, 2, and 3, respectively. A manual sequence alignment of the three regulatory boxes revealed the presence of inverted and non-inverted repeats of five to eight nucleotides, partially matching DNA binding motifs previously described for BvrR. We propose that P-BvrR binds directly to up to three regulatory boxes and probably interacts with other transcription factors to regulate\n                    <jats:italic>omp25<\/jats:italic>\n                    expression. This gene regulation model could apply to other BvrR target genes and to orthologs of the TCS BvrR\/BvrS and Omp25 in phylogenetically closed\n                    <jats:italic>Rhizobiales<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2023.1241143","type":"journal-article","created":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T02:35:18Z","timestamp":1695177318000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["The Brucella abortus two-component system response regulator BvrR binds to three DNA regulatory boxes in the upstream region of omp25"],"prefix":"10.3389","volume":"14","author":[{"given":"Amanda","family":"Castillo-Zeled\u00f3n","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Rivas-Solano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabi\u00e1n","family":"Villalta-Romero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olman","family":"G\u00f3mez-Espinoza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgardo","family":"Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esteban","family":"Chaves-Olarte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Guzm\u00e1n-Verri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,9,14]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"e0000421","DOI":"10.1128\/IAI.00004-21","article-title":"Intracellular passage triggers a molecular response in Brucella abortus that increases its infectiousness","volume":"89","author":"Altamirano-Silva","year":"2021","journal-title":"Infect. 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