{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T00:37:27Z","timestamp":1793147847453,"version":"4.5.0"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T00:00:00Z","timestamp":1696377600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Brucellosis remains a worldwide zoonotic disease with a serious impact on public health and livestock productivity. Controlling brucellosis in livestock is crucial for limiting human infections in the absence of effective human vaccines. Brucellosis control measures are majorly dependent on rigorous monitoring of disease outbreaks and mass vaccination of livestock. Live attenuated vaccines are available for livestock vaccination that play a vital role in brucellosis control programs in many countries. Even though the existing animal vaccines confer protection against brucellosis, they carry some drawbacks, including their infectivity to humans and interference with sero-monitoring. The available serodiagnostic assays for brucellosis depend on detecting anti-LPS antibodies in the serum. Since diagnosis plays a vital role in controlling brucellosis, developing improved serodiagnostic assays with enhanced specificity, sensitivity and DIVA capability is required. Therefore, it is essential to identify novel antigens for developing improved vaccines and serodiagnostic assays for brucellosis. In the present study, we performed a high throughput immunoprofiling of <jats:italic>B. melitensis<\/jats:italic> protein microarray using brucellosis-positive human and animal serum samples. The screening identified several serodominant proteins of <jats:italic>Brucella<\/jats:italic> that exhibited common or differential reactivity with sera from animals and humans. Subsequently, we cloned, expressed, and purified ten serodominant proteins, followed by analyzing their potential to develop next-generation vaccines and improved serodiagnostic assays for brucellosis. Further, we demonstrated the protective efficacy of one of the serodominant proteins against the <jats:italic>B. melitensis<\/jats:italic> challenge in mice. We found that the seroreactive protein, Dps (BMEI1980), strongly reacted with brucellosis-positive serum samples, but it did not react with sera from <jats:italic>B. abortus<\/jats:italic> S19-vaccinated cattle, indicating DIVA capability. A prototype lateral flow assay and indirect ELISA based on Dps protein exhibited high sensitivity, specificity, and DIVA capability. Thus, the present study identified promising candidates for developing improved vaccines and affordable, DIVA-capable serodiagnostic assays for animal and human brucellosis.<\/jats:p>","DOI":"10.3389\/fmicb.2023.1253349","type":"journal-article","created":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T08:46:24Z","timestamp":1696409184000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["Immuno-profiling of Brucella proteins for developing improved vaccines and DIVA capable serodiagnostic assays for brucellosis"],"prefix":"10.3389","volume":"14","author":[{"given":"Prachita","family":"Nandini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Padmaja","family":"Jakka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subathra","family":"Murugan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Varadendra","family":"Mazumdar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepak","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richa","family":"Prakash","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukhadeo B.","family":"Barbuddhe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Girish","family":"Radhakrishnan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,10,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1016\/j.vetimm.2012.01.015","article-title":"Chaperonin GroEL a brucella immunodominant antigen identified using nanobody and MALDI-TOF-MS technologies","volume":"146","author":"Abbady","year":"2012","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"28","DOI":"10.4103\/0255-0857.31058","article-title":"Brucellosis in high risk group individuals","volume":"25","author":"Agasthya","year":"2007","journal-title":"Indian J. Med. Microbiol."},{"key":"ref3","first-page":"487","article-title":"Laboratory-based diagnosis of brucellosis--a review of the literature. Part I: techniques for direct detection and identification of Brucella spp","volume":"49","author":"Al Dahouk","year":"","journal-title":"Clin. Lab."},{"key":"ref4","first-page":"577","article-title":"Laboratory-based diagnosis of brucellosis--a review of the literature. Part II: serological tests for brucellosis","volume":"49","author":"Al Dahouk","year":"","journal-title":"Clin. Lab."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"e317","DOI":"10.1371\/journal.pmed.0040317","article-title":"Perspectives for the treatment of brucellosis in the 21st century: the Ioannina recommendations","volume":"4","author":"Ariza","year":"2007","journal-title":"PLoS Med."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"3435","DOI":"10.1016\/j.vaccine.2004.02.041","article-title":"Adverse events in humans associated with accidental exposure to the livestock brucellosis vaccine RB51","volume":"22","author":"Ashford","year":"2004","journal-title":"Vaccine"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"743509","DOI":"10.1155\/2013\/743509","article-title":"A history of the development of brucella vaccines","volume":"2013","author":"Avila-Calder\u00f3n","year":"2013","journal-title":"Biomed. Res. Int."},{"key":"ref8","volume-title":"Generation of baculovirus-","author":"Bae","year":"1999"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"1631","DOI":"10.1111\/febs.14067","article-title":"Ribosomal protein L7\/L12 is required for GTPase translation factors EF-G, RF3, and IF2 to bind in their GTP state to 70S ribosomes","volume":"284","author":"Carlson","year":"2017","journal-title":"FEBS J."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/j.mcp.2009.12.002","article-title":"Recombinant OMP28 antigen-based indirect ELISA for serodiagnosis of bovine brucellosis","volume":"24","author":"Chaudhuri","year":"2010","journal-title":"Mol. Cell. Probes"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"213","DOI":"10.3201\/eid0302.970219","article-title":"Brucellosis: an overview","volume":"3","author":"Corbel","year":"1997","journal-title":"Emerg. Infect. Dis."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"859","DOI":"10.2217\/fmb.10.52","article-title":"Brucella taxonomy and evolution","volume":"5","author":"Ficht","year":"2010","journal-title":"Future Microbiol."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"2005","DOI":"10.1128\/jcm.38.5.2005-2006.2000","article-title":"Brucella abortus infection acquired in microbiology laboratories","volume":"38","author":"Fiori","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1111\/j.1365-2958.2004.04407.x","article-title":"The malaria parasite plasmodium falciparum has only one pyruvate dehydrogenase complex, which is located in the apicoplast","volume":"55","author":"Foth","year":"2005","journal-title":"Mol. Microbiol."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"1695","DOI":"10.1590\/1678-6767","article-title":"Indirect ELISA for diagnosis of Brucella ovis infection in rams","volume":"66","author":"Fran\u00e7a","year":"2014","journal-title":"Arquivo Brasileiro de Medicina Veterin\u00e1ria e Zootecnia"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1111\/j.1462-5822.2005.00502.x","article-title":"The sheathed flagellum of Brucella melitensis is involved in persistence in a murine model of infection","volume":"7","author":"Fretin","year":"2005","journal-title":"Cell. Microbiol."},{"key":"ref17","first-page":"233","article-title":"Brucellosis in humans--etiology, diagnostics, clinical forms","volume":"20","author":"Gali\u0144ska","year":"2013","journal-title":"Ann. Agricult. Environ. Med."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"296","DOI":"10.3325\/cmj.2010.51.296","article-title":"Diagnosis of brucellosis in livestock and wildlife","volume":"51","author":"Godfroid","year":"2010","journal-title":"Croat. Med. J."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1016\/j.prevetmed.2011.04.007","article-title":"Brucellosis at the animal\/ecosystem\/human interface at the beginning of the 21st century","volume":"102","author":"Godfroid","year":"2011","journal-title":"Prev. Vet. Med."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/s1286-4579(00)01350-2","article-title":"Immunity and protection against Brucella abortus","volume":"3","author":"Golding","year":"2001","journal-title":"Microb. Infect. Institut Pasteur."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"e55376","DOI":"10.15252\/embr.202255376","article-title":"Intracellular growth of brucella is mediated by Dps-dependent activation of ferritinophagy","volume":"24","author":"Hop","year":"2023","journal-title":"EMBO Rep."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"13906","DOI":"10.1074\/jbc.M113.459305","article-title":"The Brucella abortus general stress response system regulates chronic mammalian infection and is controlled by phosphorylation and proteolysis","volume":"288","author":"Kim","year":"2013","journal-title":"J. Biol. Chem."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"1059","DOI":"10.1292\/jvms.66.1059","article-title":"Zinc uptake system (znuA locus) of Brucella abortus is essential for intracellular survival and virulence in mice","volume":"66","author":"Kim","year":"2004","journal-title":"J. Vet. Med. Sci. Japanese Soc. Vet. Sci."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1128\/CMR.16.1.65-78.2003","article-title":"Molecular host-pathogen interaction in brucellosis: current understanding and future approaches to vaccine development for mice and humans","volume":"16","author":"Ko","year":"2003","journal-title":"Clin. Microbiol. Rev."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"1594","DOI":"10.1021\/pr800978p","article-title":"Intracellular adaptation of Brucella abortus","volume":"8","author":"Lamontagne","year":"2009","journal-title":"J. Proteome Res."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1099\/0022-1317-51-1-20","article-title":"Induction of a th1-type of immune response but not protective immunity by intramuscular DNA immunisation with Brucella abortus GroEL heat-shock gene","volume":"51","author":"Leclerq","year":"2002","journal-title":"J. Med. Microbiol."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"e673","DOI":"10.1371\/journal.pntd.0000673","article-title":"Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens","volume":"4","author":"Liang","year":"2010","journal-title":"PLoS Negl. Trop. Dis."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1016\/S0255-0857(21)02105-8","article-title":"Review of clinical and laboratory features of human brucellosis","volume":"25","author":"Mantur","year":"2007","journal-title":"Indian J. Med. Microbiol."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"5188","DOI":"10.1128\/jb.179.16.5188-5194.1997","article-title":"Protection of DNA during oxidative stress by the nonspecific DNA-binding protein Dps","volume":"179","author":"Martinez","year":"1997","journal-title":"J. Bacteriol."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"249","DOI":"10.20506\/rst.32.1.2197","article-title":"Economics of brucellosis impact and control in low-income countries","volume":"32","author":"Mcdermott","year":"2013","journal-title":"Rev. Sci. Tech."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"65","DOI":"10.3325\/cmj.2010.51.306","article-title":"Serological diagnosis of brucellosis. Prilozi \/ Makedonska akademija na naukite i umetnostite, Oddelenie za bioloski i medicinski nauki = contributions \/ Macedonian Academy of Sciences and arts","volume":"31","author":"Nielsen","year":"2010","journal-title":"Section Biol. Med. Sci."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"5201","DOI":"10.1128\/iai.62.11.5201-5204.1994","article-title":"Subcloning and expression of the Brucella abortus L7\/L12 ribosomal gene and T-lymphocyte recognition of the recombinant protein","volume":"62","author":"Oliveira","year":"1994","journal-title":"Infect. Immun."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"959","DOI":"10.1016\/0264-410x(96)00018-7","article-title":"Immunization of mice with recombinant L7\/L12 ribosomal protein confers protection against Brucella abortus infection","volume":"14","author":"Oliveira","year":"1996","journal-title":"Vaccine"},{"key":"ref34","first-page":"659","article-title":"Recombinant L7\/L12 ribosomal protein and gamma-irradiated Brucella abortus induce a T-helper 1 subset response from murine CD4+ T cells","volume":"83","author":"Oliveira","year":"1994","journal-title":"Immunology"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"46","DOI":"10.2174\/1874318801004010046","article-title":"Diagnosis of brucellosis","volume":"4","author":"Padilla Poester","year":"2010","journal-title":"Open Vet. Sci. J."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"820","DOI":"10.1002\/pmic.201100185","article-title":"Immunoproteomics of Brucella abortus reveals differential antibody profiles between S19-vaccinated and naturally infected cattle","volume":"12","author":"Pajuaba","year":"2012","journal-title":"Proteomics"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/s1473-3099(06)70382-6","article-title":"The new global map of human brucellosis","volume":"6","author":"Pappas","year":"2006","journal-title":"Lancet Infect. Dis."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1128\/iai.01151-08","article-title":"Immunization with recombinant brucella species outer membrane protein Omp16 or Omp19 in adjuvant induces specific CD4+ and CD8+ T cells as well as systemic and oral protection against Brucella abortus infection","volume":"77","author":"Pasquevich","year":"2009","journal-title":"Infect. Immun."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1146\/annurev.micro.57.030502.090803","article-title":"Brucella stationary-phase gene expression and virulence","volume":"57","author":"Roop","year":"2003","journal-title":"Annu. Rev. Microbiol."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1097\/QCO.0b013e3283638104","article-title":"Brucellosis in low-income and middle-income countries","volume":"26","author":"Rubach","year":"2013","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.1016\/j.vaccine.2011.12.088","article-title":"Oral immunization of mice with recombinant Lactococcus lactis expressing cu, Zn superoxide dismutase of Brucella abortus triggers protective immunity","volume":"30","author":"S\u00e1ez","year":"2012","journal-title":"Vaccine"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"2090","DOI":"10.1099\/ijsem.0.000998","article-title":"Brucella vulpis sp. nov., isolated from mandibular lymph nodes of red foxes (Vulpes vulpes)","volume":"66","author":"Scholz","year":"2016","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1016\/s0378-1135(02)00255-9","article-title":"Brucellosis vaccines: past, present and future","volume":"90","author":"Schurig","year":"2002","journal-title":"Vet. Microbiol."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"2786","DOI":"10.1016\/j.vaccine.2015.04.030","article-title":"Recombinant L7\/L12 protein entrapping PLGA (poly lactide-co-glycolide) micro particles protect BALB\/c mice against the virulent B. abortus 544 infection","volume":"33","author":"Singh","year":"2015","journal-title":"Vaccine"},{"key":"ref45","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/j.micinf.2010.10.015","article-title":"Cell-mediated immunity in human brucellosis","volume":"13","author":"Skendros","year":"2011","journal-title":"Microb. infect. Institut Pasteur."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"245","DOI":"10.2165\/00003495-199753020-00005","article-title":"Recognition and optimum treatment of brucellosis","volume":"53","author":"Solera","year":"1997","journal-title":"Drugs"},{"key":"ref47","doi-asserted-by":"publisher","first-page":"2954","DOI":"10.1128\/iai.68.5.2954-2961.2000","article-title":"Characterization of heat, oxidative, and acid stress responses in Brucella melitensis","volume":"68","author":"Teixeira-Gomes","year":"2000","journal-title":"Infect. Immun."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1099\/jmm.0.017566-0","article-title":"Cloning and expression of the immunoreactive Brucella melitensis 28 kDa outer-membrane protein (Omp28) encoding gene and evaluation of the potential of Omp28 for clinical diagnosis of brucellosis","volume":"59","author":"Thavaselvam","year":"2010","journal-title":"J. Med. Microbiol."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"3740","DOI":"10.4049\/jimmunol.1302561","article-title":"Humoral immunity and CD4+ Th1 cells are both necessary for a fully protective immune response upon secondary infection with Brucella melitensis","volume":"192","author":"Vitry","year":"2014","journal-title":"J. Immunol. (Baltimore, Md: 1950)"},{"key":"ref50","doi-asserted-by":"publisher","first-page":"1047","DOI":"10.1002\/1615-9861(200208)2:8<1047::Aid-prot1047>3.0.Co;2-8","article-title":"Global analysis of the Brucella melitensis proteome: identification of proteins expressed in laboratory-grown culture","volume":"2","author":"Wagner","year":"2002","journal-title":"Proteomics"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1007\/s11515-012-1196-0","article-title":"Progress in Brucella vaccine development","volume":"8","author":"Yang","year":"2013","journal-title":"Front. Biol."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1080\/713608012","article-title":"T cell immunity to brucellosis","volume":"29","author":"Yingst","year":"2003","journal-title":"Crit. Rev. 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