{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:50:58Z","timestamp":1787244658405,"version":"build-2736575974"},"reference-count":35,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Foodborne illness outbreaks in the U.S. associated with consumption of both fresh and dried specialty mushrooms have recently occurred. Dried wood ear mushrooms were implicated in a salmonellosis outbreak in 2020, while fresh enoki mushrooms were associated with two listeriosis outbreaks in 2020 and 2023. These specialty mushrooms are commercially available in both their fresh and dried states. Due to the short shelf life of mushrooms, dehydration is a common method used in both industry and by consumers to extend the shelf life and preserve quality. Therefore, the aim of this study was to evaluate the use of dehydration on the inactivation kinetics of both <jats:italic>Listeria monocytogenes<\/jats:italic> and <jats:italic>Salmonella enterica<\/jats:italic> on enoki and wood ear mushrooms. Fresh mushrooms were inoculated with four strain cocktails of either <jats:italic>L. monocytogenes<\/jats:italic> or <jats:italic>S. enterica<\/jats:italic> and dried at ambient conditions for 10\u2009min. Following drying of the inoculum, mushrooms were placed into food dehydrators preheated to 70, 80, or 90\u00b0C and treated for up to 24\u2009h. At treatment intervals, mushrooms were removed from the dehydrators for pathogen enumeration. Inactivation kinetics for both pathogens were modeled using the Weibull, log-linear with tail, and log-linear with shoulder models. Pathogen reductions of &amp;gt;4 log CFU\/g were achieved on both enoki and wood ear mushrooms during dehydration at 90\u00b0C after only 2\u20134\u2009h. At 70 and 80\u00b0C, log reductions of &amp;gt;4 log CFU\/g were observed on wood ear mushrooms after 4\u20138\u2009h. On enoki mushrooms, a tailing effect was observed with residual populations (&amp;gt;2 log CFU\/g) of <jats:italic>L. monocytogenes<\/jats:italic> and <jats:italic>S. enterica<\/jats:italic> remaining even after 24\u2009h of treatment at both 70 and 80\u00b0C. This study emphasizes the need for an individualized dehydration strategy for each mushroom type to ensure the effectiveness of dehydration as a process to reduce pathogen populations. Results of this study will aid in informing proper time and temperature combinations for dehydration of specialty mushrooms to ensure product safety.<\/jats:p>","DOI":"10.3389\/fmicb.2023.1257053","type":"journal-article","created":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T10:01:00Z","timestamp":1698746460000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Effect of dehydration on the inactivation of Listeria monocytogenes and Salmonella enterica on enoki and wood ear mushrooms"],"prefix":"10.3389","volume":"14","author":[{"given":"Joelle K.","family":"Salazar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan L.","family":"Fay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bashayer A.","family":"Khouja","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirali J.","family":"Chavda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gayatri R.","family":"Patil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David T.","family":"Ingram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,10,31]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1056","DOI":"10.1111\/1541-4337.12224","article-title":"Performance of drying technologies to ensure microbial safety of dried fruits and vegetables","volume":"15","author":"Bourdoux","year":"2016","journal-title":"Compr. 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Food Prot."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"500","DOI":"10.1016\/j.fm.2006.09.004","article-title":"Influence of modified blanching treatments on inactivation of Salmonella during drying and storage of carrot slices","volume":"24","author":"Dipersio","year":"2007","journal-title":"Food Microbiol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1016\/j.ijfoodmicro.2013.07.007","article-title":"Modeling the influence of temperature, water activity and water mobility on the persistence of Salmonella in low-moisture foods","volume":"166","author":"Farakos","year":"2013","journal-title":"Int. J. Food Microbiol."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"879","DOI":"10.4315\/0362-028X.JFP-16-392","article-title":"Salmonella survival kinetics on pecans, hazelnuts, and pine nuts at various water activities and temperatures","volume":"80","author":"Farakos","year":"2017","journal-title":"J. Food Prot."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"104304","DOI":"10.1016\/j.fm.2023.104304","article-title":"Survival kinetics of Listeria monocytogenes and Salmonella enterica on dehydrated enoki and wood ear mushrooms during long-term storage","volume":"114","author":"Fay","year":"2023","journal-title":"Food Microbiol."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1016\/j.foodchem.2012.04.136","article-title":"Effect of gamma and electron beam irradiation on the physico-chemical and nutritional properties of mushrooms: a review","volume":"135","author":"Fernandes","year":"2012","journal-title":"Food Chem."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/S0168-1605(00)00362-7","article-title":"Structural model requirements to describe microbial inactivation during a mild heat treatment","volume":"59","author":"Geeraerd","year":"2000","journal-title":"Int. J. Food Microbiol."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.ijfoodmicro.2004.11.038","article-title":"GInaFiT, a freeware tool to assess non-log-linear microbial survivor curves","volume":"102","author":"Geeraerd","year":"2005","journal-title":"Int. J. Food Microbiol."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"108715","DOI":"10.1016\/j.foodcont.2021.108715","article-title":"Survival of Listeria monocytogenes, Bacillus cereus and Salmonella Typhimurium on sliced mushrooms during drying in a household food dehydrator","volume":"134","author":"Kragh","year":"2022","journal-title":"Food Control"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"1739","DOI":"10.1021\/acs.jafc.0c05934","article-title":"Review on Auricularia auricula-judae as a functional food: growth, chemical composition, and biological activities","volume":"69","author":"Liu","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/S0168-1605(01)00624-9","article-title":"On calculating sterility in thermal preservation methods: application of the Weibull frequency distribution model","volume":"72","author":"Mafart","year":"2002","journal-title":"Int. J. Food Microbiol."},{"key":"ref18","year":"2017"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"1988","DOI":"10.4315\/0362-028X.JFP-15-195","article-title":"Predominance and distribution of a persistent Listeria monocytogenes clone in a commercial fresh mushroom processing environment","volume":"78","author":"Murugesan","year":"2015","journal-title":"J. Food Prot."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.jfoodeng.2005.02.008","article-title":"Effects of pre-treatments and drying temperatures on the quality of dried button mushrooms","volume":"2","author":"Nour","year":"2011","journal-title":"South West J. Hortic. Biol. Environ."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"100101","DOI":"10.1016\/j.jfp.2023.100101","article-title":"Multinational outbreak of Listeria monocytogenes infections linked to Enoki mushrooms imported from the Republic of Korea 2016-2020","volume":"86","author":"Pereira","year":"2023","journal-title":"J. Food Prot."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"1511","DOI":"10.1002\/jsfa.8622","article-title":"Volatile composition and sensory profile of shiitake mushrooms as affected by drying method","volume":"98","author":"Politowicz","year":"","journal-title":"J. Sci. Food Agric."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1080\/07373937.2016.1274903","article-title":"Volatile composition and sensory profile of oyster mushroom as affected by drying method","volume":"36","author":"Politowicz","year":"","journal-title":"Dry. Technol."},{"key":"ref24","first-page":"462","article-title":"Miltistate outbreak of Salmonella serotype Agona infections linked to toasted oats cereal - United States, April-may, 1998","volume":"47","year":"1998","journal-title":"Morb. Mortal. Wkly Rep."},{"key":"ref25","year":""},{"key":"ref26","volume-title":"Outbreak of","year":""},{"key":"ref27","year":"2023"},{"key":"ref28","year":""},{"key":"ref29","year":""},{"key":"ref30","year":""},{"key":"ref31","volume-title":"FDA issues country-wide import alert for Enoki mushrooms from the Republic of Korea","year":"2022"},{"key":"ref32","year":"2020"},{"key":"ref33","year":"1994"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"1492","DOI":"10.1016\/j.fm.2011.08.007","article-title":"Microbiological quality and safety of fresh cultivated and wild mushrooms commercialized in Spain","volume":"28","author":"Venturini","year":"2011","journal-title":"Food Microbiol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"608","DOI":"10.4315\/0362-028X.JFP-12-292","article-title":"Incidence of Listeria monocytogenes and Listeria spp. in a small-scale mushroom production facility","volume":"76","author":"Viswanath","year":"2013","journal-title":"J. Food Prot."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"108935","DOI":"10.1016\/j.foodcont.2022.108935","article-title":"Survivability of Salmonella and Enterococcus faecium in chili, cinnamon and black pepper powders during storage and isothermal treatments","volume":"137","author":"Xie","year":"2022","journal-title":"Food Control"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2023.1257053\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T10:01:03Z","timestamp":1698746463000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2023.1257053\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,31]]},"references-count":35,"alternative-id":["10.3389\/fmicb.2023.1257053"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2023.1257053","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,31]]},"article-number":"1257053"}}