{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T14:48:40Z","timestamp":1793198920371,"version":"4.5.0"},"reference-count":94,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T00:00:00Z","timestamp":1702512000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Three novel strains designated ABR2-5<jats:sup>T<\/jats:sup>, BKB1-1<jats:sup>T<\/jats:sup>, and WSW4-B4<jats:sup>T<\/jats:sup> belonging to the genus <jats:italic>Reichenbachiella<\/jats:italic> of the phylum <jats:italic>Bacteroidota<\/jats:italic> were isolated from algae and mud samples collected in the West Sea, Korea. All three strains were enriched for genes encoding up to 216 carbohydrate-active enzymes (CAZymes), which participate in the degradation of agar, alginate, carrageenan, laminarin, and starch. The 16S rRNA sequence similarities among the three novel isolates were 94.0%\u201394.7%, and against all three existing species in the genus <jats:italic>Reichenbachiella<\/jats:italic> they were 93.6%\u201397.2%. The genome sizes of the strains ABR2-5<jats:sup>T<\/jats:sup>, BKB1-1<jats:sup>T<\/jats:sup>, and WSW4-B4<jats:sup>T<\/jats:sup> were 5.5, 4.4, and 5.0\u2009Mb, respectively, and the GC content ranged from 41.1%\u201342.0%. The average nucleotide identity and the digital DNA\u2013DNA hybridization values of each novel strain within the isolates and all existing species in the genus <jats:italic>Reichenbachiella<\/jats:italic> were in a range of 69.2%\u201375.5% and 17.7\u201318.9%, respectively, supporting the creation of three new species. The three novel strains exhibited a distinctive fatty acid profile characterized by elevated levels of iso-C<jats:sub>15:0<\/jats:sub> (37.7%\u201347.4%) and C<jats:sub>16:1<\/jats:sub> \u03c95c (14.4%\u201322.9%). Specifically, strain ABR2-5<jats:sup>T<\/jats:sup> displayed an additional higher proportion of C<jats:sub>16:0<\/jats:sub> (13.0%). The polar lipids were phosphatidylethanolamine, unidentified lipids, aminolipids, and glycolipids. Menaquinone-7 was identified as the respiratory quinone of the isolates. A comparative genome analysis was performed using the KEGG, RAST, antiSMASH, CRISPRCasFinder, dbCAN, and dbCAN-PUL servers and CRISPRcasIdentifier software. The results revealed that the isolates harbored many key genes involved in central metabolism for the synthesis of essential amino acids and vitamins, hydrolytic enzymes, carotenoid pigments, and antimicrobial compounds. The KEGG analysis showed that the three isolates possessed a complete pathway of dissimilatory nitrate reduction to ammonium (DNRA), which is involved in the conservation of bioavailable nitrogen within the ecosystem. Moreover, all the strains possessed genes that participated in the metabolism of heavy metals, including arsenic, copper, cobalt, ferrous, and manganese. All three isolated strains contain the class 2 type II subtype C1 CRISPR-Cas system in their genomes. The distinguished phenotypic, chemotaxonomic, and genomic characteristics led us to propose that the three strains represent three novel species in the genus <jats:italic>Reichenbachiella<\/jats:italic>: <jats:italic>R. ulva<\/jats:italic>e sp. nov. (ABR2-5<jats:sup>T<\/jats:sup>\u2009=\u2009KCTC 82990<jats:sup>T<\/jats:sup>\u2009=\u2009JCM 35839<jats:sup>T<\/jats:sup>), <jats:italic>R. agarivorans<\/jats:italic> sp. nov. (BKB1-1<jats:sup>T<\/jats:sup>\u2009=\u2009KCTC 82964<jats:sup>T<\/jats:sup>\u2009=\u2009JCM 35840<jats:sup>T<\/jats:sup>), and <jats:italic>R. carrageenanivorans<\/jats:italic> sp. nov. (WSW4-B4<jats:sup>T<\/jats:sup>\u2009=\u2009KCTC 82706<jats:sup>T<\/jats:sup>\u2009=\u2009JCM 35841<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.3389\/fmicb.2023.1265676","type":"journal-article","created":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T15:53:33Z","timestamp":1702569213000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Three novel marine species of the genus Reichenbachiella exhibiting degradation of complex polysaccharides"],"prefix":"10.3389","volume":"14","author":[{"given":"Neak","family":"Muhammad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Forbes","family":"Avila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga I.","family":"Nedashkovskaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song-Gun","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,12,14]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"72","DOI":"10.4056\/sigs.1543845","article-title":"Complete genome sequence of Cellulophaga algicola type strain (IC166T)","volume":"4","author":"Abt","year":"2011","journal-title":"Stand Genomic Sci."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1186\/1471-2180-12-193","article-title":"Characterization of copper-resistant bacteria and bacterial communities from copper-polluted agricultural soils of Central Chile","volume":"12","author":"Altimira","year":"2012","journal-title":"BMC Microbiol."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1186\/s12934-022-01818-0","article-title":"Genome sequence and carbohydrate active enzymes (CAZymes) repertoire of the thermophilic Caldicoprobacter algeriensis TH7C1T","volume":"21","author":"Ameri","year":"2022","journal-title":"Microb. 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