{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T22:55:07Z","timestamp":1792450507153,"version":"4.5.0"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:00:00Z","timestamp":1695686400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Temporin-GHa obtained from the frog\n                      <jats:italic>Hylarana guentheri<\/jats:italic>\n                      showed bactericidal efficacy against\n                      <jats:italic>Streptococcus mutans<\/jats:italic>\n                      . To enhance its antibacterial activity, the derived peptides GHaR and GHa11R were designed, and their antibacterial performance, antibiofilm efficacy and potential in the inhibition of dental caries were evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Bacterial survival assay, fluorescent staining assay and transmission electron microscopy observation were applied to explore how the peptides inhibited and killed\n                      <jats:italic>S. mutans<\/jats:italic>\n                      . The antibiofilm efficacy was assayed by examining exopolysaccharide (EPS) and lactic acid production, bacterial adhesion and cell surface hydrophobicity. The gene expression level of virulence factors of\n                      <jats:italic>S. mutans<\/jats:italic>\n                      was detected by qRT-PCR. Finally, the impact of the peptides on the caries induced ability of\n                      <jats:italic>S. mutans<\/jats:italic>\n                      was measured using a rat caries model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      It has been shown that the peptides inhibited biofilm rapid accumulation by weakening the initial adhesion of\n                      <jats:italic>S. mutans<\/jats:italic>\n                      and reducing the production of EPS. Meanwhile, they also decreased bacterial acidogenicity and aciduricity, and ultimately prevented caries development in vivo.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      GHaR and GHa11R might be promising candidates for controlling\n                      <jats:italic>S. mutans<\/jats:italic>\n                      infections.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2023.1267389","type":"journal-article","created":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T05:09:25Z","timestamp":1695791365000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Antimicrobial peptide temporin derivatives inhibit biofilm formation and virulence factor expression of Streptococcus mutans"],"prefix":"10.3389","volume":"14","author":[{"given":"Shangjun","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanmei","family":"Zha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiying","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangshuang","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanting","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lushuang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junchen","family":"Lyu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenting","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daqi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manchuriga","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingxia","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,9,26]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1016\/j.biochi.2012.10.015","article-title":"Antibacterial and leishmanicidal activities of temporin-SHd, a 17-residue long membrane-damaging peptide.","volume":"95","author":"Abbassi","year":"2013","journal-title":"Biochimie"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3390\/biom10050811","article-title":"Hindering of cariogenic Streptococcus mutans biofilm by fatty acid array derived from an endophytic Arthrographis kalrae strain.","volume":"10","author":"Abdel-Aziz","year":"2020","journal-title":"Biomolecules"},{"key":"B3","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1159\/000516175","article-title":"Streptococcus salivarius isolates of varying acid tolerance exhibit F1F0-ATPase conservation.","volume":"55","author":"Allen","year":"2021","journal-title":"Caries Res."},{"key":"B4","doi-asserted-by":"publisher","first-page":"10","DOI":"10.3390\/microorganisms9102031","article-title":"Anti-biofilm activity of cannabigerol against Streptococcus mutans","volume":"9","author":"Aqawi","year":"2021","journal-title":"Microorganisms"},{"key":"B5","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1016\/0003-2697(83)90005-2","article-title":"New assay for enzymatic phosphate release: Application to aspartate transcarbamylase and other enzymes.","volume":"132","author":"Bencini","year":"1983","journal-title":"Anal. 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