{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:51:43Z","timestamp":1789087903675,"version":"build-2803163510"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,12,22]],"date-time":"2023-12-22T00:00:00Z","timestamp":1703203200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    The study evaluated the association of DNase I enzyme with antimicrobial photodynamic therapy (aPDT) in the treatment of oral candidiasis in mice infected with fluconazole-susceptible (CaS) and -resistant (CaR)\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    strains. Mice were inoculated with\n                    <jats:italic>C. albicans<\/jats:italic>\n                    , and after the infection had been established, the tongues were exposed to DNase for 5\u2009min, followed by photosensitizer [Photodithazine\n                    <jats:sup>\u00ae<\/jats:sup>\n                    (PDZ)] and light (LED), either singly or combined. The treatments were performed for 5 consecutive days. Treatment efficacy was evaluated by assessing the tongues via fungal viable population, clinical evaluation, histopathological and fluorescence microscopy methods immediately after finishing treatments, and 7\u2009days of follow-up. The combination of DNase with PDZ-aPDT reduced the fungal viability in mice tongues immediately after the treatments by around 4.26 and 2.89 log\n                    <jats:sub>10<\/jats:sub>\n                    for CaS and CaR, respectively (versus animals only inoculated). In the fluorescence microscopy, the polysaccharides produced by\n                    <jats:italic>C. albicans<\/jats:italic>\n                    and fungal cells were less labeled in animals treated with the combination of DNase with PDZ-aPDT, similar to the healthy animals. After 7\u2009days of the treatment, DNase associated with PDZ-aPDT maintained a lower count, but not as pronounced as immediately after the intervention. For both strains, mice treated with the combination of DNase with PDZ-aPDT showed remission of oral lesions and mild inflammatory infiltrate in both periods assessed, while animals treated only with PDZ-aPDT presented partial remission of oral lesions. DNase I enzyme improved the efficacy of photodynamic treatment.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2023.1274201","type":"journal-article","created":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T23:21:48Z","timestamp":1703200908000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["DNase improves the efficacy of antimicrobial photodynamic therapy in the treatment of candidiasis induced with Candida albicans"],"prefix":"10.3389","volume":"14","author":[{"given":"Cl\u00e1udia Carolina","family":"Jord\u00e3o","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlise In\u00eaz","family":"Klein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Aboud","family":"Barbugli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewerton Garcia de Oliveira","family":"Mima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00e1bata Viana","family":"de Sousa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00falio Morandin","family":"Ferrisse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Claudia","family":"Pavarina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,12,22]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1855","DOI":"10.1111\/odi.14149","article-title":"DNase enhances photodynamic therapy against fluconazole-resistant Candida albicans biofilms","volume":"29","author":"Abreu-Pereira","year":"2022","journal-title":"Oral Dis."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1136\/pmj.78.922.455","article-title":"Oral candidiasis","volume":"78","author":"Akpan","year":"2002","journal-title":"Postgrad. 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