{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T15:47:32Z","timestamp":1766159252603,"version":"3.41.2"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T00:00:00Z","timestamp":1696291200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016104","name":"Key Project of Research and Development Plan of Hunan Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016104","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The intestinal microbiota is known to play a role in the development of liver disease, there is a limited understanding of the intestinal microbiota associated with chronic schistosomiasis <jats:italic>japonica<\/jats:italic>. This study sought to explore the characteristics of the intestinal microbiota in patients with chronic schistosomiasis <jats:italic>japonica<\/jats:italic> and identify potential biomarkers that could aid diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 40 residents of Qingshan Island in Yueyang (Hunan, China) were enrolled in this cross-sectional study. These individuals were divided into two groups for analysis of the intestinal microbiota: patients with chronic schistosomiasis <jats:italic>japonica<\/jats:italic>-induced liver fibrosis group (CSJ group, <jats:italic>n<\/jats:italic> = 10) and a healthy control group (HC group, <jats:italic>n<\/jats:italic> = 30). Feces were collected from each participant and analyzed by 16S rRNA gene sequencing, which included species composition analysis at the phylum and family levels, \u03b1 and \u03b2 diversity analysis, LEfSe, Kyoto Encyclopedia of Genes and Genome (KEGG) and Clusters of Orthologous Groups of proteins (COG) analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results indicated that <jats:italic>Schistosoma japonicum<\/jats:italic> infection changed the composition and abundance of intestinal microbiota at the phylum and family levels. Compared with the HC group, the \u03b1 and \u03b2 diversity results showed that CSJ group had low diversity of species of the intestinal microbiome. LEfSe and relative abundance analysis found that the <jats:italic>Prevotella<\/jats:italic> 7, <jats:italic>Alloprevotella<\/jats:italic>, and <jats:italic>Holdemanella<\/jats:italic> genera were significantly higher in the CSJ group than in the HC group. Meanwhile, the ROC analysis showed that the area under the curve (AUC) of <jats:italic>Prevotella<\/jats:italic> 7, <jats:italic>Alloprevotella<\/jats:italic>, and <jats:italic>Holdemanella<\/jats:italic> genera was 0.779, 0.769, and 0.840, respectively. KEGG and COG analysis showed that the Replication and Repair, and Defense Mechanism pathways correlated strongly with chronic schistosomiasis <jats:italic>japonica<\/jats:italic> infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The current study was the first to explore differences in the intestinal microbiota of patients with chronic schistosomiasis <jats:italic>japonica<\/jats:italic>-induced liver fibrosis and healthy people from Qingshan Island, which indicated that <jats:italic>Prevotella<\/jats:italic> 7, <jats:italic>Alloprevotella<\/jats:italic>, and <jats:italic>Holdemanella<\/jats:italic> genera could have a potential value in non-invasive diagnosis of chronic schistosomiasis <jats:italic>japonica<\/jats:italic>-induced fibrosis.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2023.1276404","type":"journal-article","created":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T08:04:36Z","timestamp":1696320276000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["The characteristics of intestinal microbiota in patients with chronic schistosomiasis japonica-induced liver fibrosis by 16S rRNA gene sequence"],"prefix":"10.3389","volume":"14","author":[{"given":"Chen","family":"Guo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengpeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junhui","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulin","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingzi","family":"Ming","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2023,10,3]]},"reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/0001-706X(92)90020-X","article-title":"The use of diagnostic ultrasound in schistosomiasis\u2013attempts at standardization of methodology. Cairo Working Group","volume":"51","author":"Abdel Wahab","year":"1992","journal-title":"Acta Trop."},{"key":"B2","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/j.immuni.2017.04.008","article-title":"Homeostatic immunity and the microbiota.","volume":"46","author":"Belkaid","year":"2017","journal-title":"Immunity"},{"key":"B3","doi-asserted-by":"crossref","DOI":"10.1186\/s40249-017-0370-7","article-title":"Elimination of schistosomiasis: The tools required.","volume":"6","author":"Bergquist","year":"2017","journal-title":"Infect Dis Poverty"},{"key":"B4","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME allows analysis of high-throughput community sequencing data.","volume":"7","author":"Caporaso","year":"2010","journal-title":"Nat Methods"},{"key":"B5","doi-asserted-by":"crossref","DOI":"10.1016\/j.actatropica.2020.105743","article-title":"Reviews and advances in diagnostic research on Schistosoma japonicum.","volume":"213","author":"Chen","year":"2021","journal-title":"Acta Trop."},{"key":"B6","doi-asserted-by":"publisher","first-page":"623","DOI":"10.17219\/acem\/146320","article-title":"The influence of mosapride on gut microbiota of carbon tetrachloride-induced cirrhosis rats based on 16S rRNA gene sequencing.","volume":"31","author":"Chen","year":"2022","journal-title":"Adv. Clin. Exp. Med."},{"key":"B7","doi-asserted-by":"crossref","DOI":"10.1186\/2049-9957-3-6","article-title":"Assessment of morbidity due to Schistosoma japonicum infection in China.","volume":"3","author":"Chen","year":"2014","journal-title":"Infect. Dis. Poverty"},{"key":"B8","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1016\/S0140-6736(13)61949-2","article-title":"Human schistosomiasis.","volume":"383","author":"Colley","year":"2014","journal-title":"Lancet"},{"key":"B9","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1093\/bioinformatics\/btq461","article-title":"Search and clustering orders of magnitude faster than BLAST.","volume":"26","author":"Edgar","year":"2010","journal-title":"Bioinformatics"},{"key":"B10","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1038\/nmeth.2604","article-title":"UPARSE: highly accurate OTU sequences from microbial amplicon reads.","volume":"10","author":"Edgar","year":"2013","journal-title":"Nat. Methods"},{"key":"B11","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1093\/bib\/bbx117","article-title":"Microbial genome analysis: the COG approach.","volume":"20","author":"Galperin","year":"2019","journal-title":"Brief Bioinform."},{"key":"B12","doi-asserted-by":"publisher","first-page":"1260","DOI":"10.1016\/S0140-6736(17)32130-X","article-title":"Global, regional, and national disability-adjusted life-years (DALYs) for 333 diseases and injuries and healthy life expectancy (HALE) for 195 countries and territories, 1990-2016: A systematic analysis for the Global Burden of Disease Study 2016.","volume":"390","year":"2017","journal-title":"Lancet"},{"key":"B13","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1016\/j.kint.2023.03.024","article-title":"Microbiome modulation after severe acute kidney injury accelerates functional recovery and decreases kidney fibrosis.","volume":"104","author":"Gharaie","year":"2023","journal-title":"Kidney Int."},{"key":"B14","doi-asserted-by":"crossref","DOI":"10.1172\/JCI143774","article-title":"Targeting the gut to treat multiple sclerosis.","volume":"131","author":"Ghezzi","year":"2021","journal-title":"J. Clin. Invest."},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-021-00821-8","article-title":"Gut microbiota signatures in Schistosoma japonicum infection-induced liver cirrhosis patients: a case-control study.","volume":"10","author":"Gui","year":"2021","journal-title":"Infect. Dis. Poverty"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2020074","article-title":"Alteration of the fecal microbiota in Chinese patients with Schistosoma japonicum infection.","volume":"28","author":"Jiang","year":"2021","journal-title":"Parasite"},{"key":"B17","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1146\/annurev-pathol-030320-095722","article-title":"Gut Microbiota in Intestinal and Liver Disease.","volume":"16","author":"Jones","year":"2021","journal-title":"Annu. Rev. Pathol."},{"key":"B18","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: kyoto encyclopedia of genes and genomes.","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"B19","doi-asserted-by":"publisher","first-page":"955","DOI":"10.1002\/hep.32197","article-title":"Gut microbiome features associated with liver fibrosis in Hispanics, a population at high risk for fatty liver disease.","volume":"75","author":"Kwan","year":"2022","journal-title":"Hepatology"},{"key":"B20","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1038\/nbt.2676","article-title":"Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences.","volume":"31","author":"Langille","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1038\/s41575-019-0258-z","article-title":"Gut microbiota-derived metabolites as key actors in inflammatory bowel disease.","volume":"17","author":"Lavelle","year":"2020","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1038\/nrgastro.2016.4","article-title":"Gut microbiota in 2015: Prevotella in the gut: choose carefully.","volume":"13","author":"Ley","year":"2016","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.574136","article-title":"Current status of schistosomiasis control and prospects for elimination in the dongting lake region of the People\u2019s Republic of China.","volume":"11","author":"Li","year":"2020","journal-title":"Front. Immunol."},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.916061","article-title":"Gut microbiome signatures in the progression of hepatitis B virus-induced liver disease.","volume":"13","author":"Li","year":"2022","journal-title":"Front. Microbiol."},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.766205","article-title":"Attenuates Hepatic and Intestinal Injuries and Modulates Gut Microbiota and Gene Expression Profiles in Mice Infected with.","volume":"9","author":"Lin","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12778","article-title":"Granuloma formation and tissue pathology in Schistosoma japonicum versus Schistosoma mansoni infections.","volume":"43","author":"Llanwarne","year":"2021","journal-title":"Parasite Immunol."},{"key":"B27","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/978-3-030-18616-6_3","article-title":"Schistosomiasis.","volume":"1154","author":"LoVerde","year":"2019","journal-title":"Adv. Exp. Med. Biol."},{"key":"B28","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1038\/ismej.2010.133","article-title":"UniFrac: an effective distance metric for microbial community comparison.","volume":"5","author":"Lozupone","year":"2011","journal-title":"ISME J."},{"key":"B29","doi-asserted-by":"publisher","first-page":"2957","DOI":"10.1093\/bioinformatics\/btr507","article-title":"FLASH: fast length adjustment of short reads to improve genome assemblies.","volume":"27","author":"Mago\u00e8","year":"2011","journal-title":"Bioinformatics"},{"journal-title":"Biology of schistosome and control of schistosomiasis.","year":"1990","author":"Mao","key":"B30"},{"key":"B31","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1007\/s00281-020-00789-x","article-title":"Schistosomiasis-from immunopathology to vaccines.","volume":"42","author":"McManus","year":"2020","journal-title":"Semin. Immunopathol."},{"key":"B32","doi-asserted-by":"crossref","DOI":"10.1038\/s41572-018-0013-8","article-title":"Schistosomiasis.","volume":"4","author":"McManus","year":"2018","journal-title":"Nat. Rev. Dis. Primers"},{"key":"B33","doi-asserted-by":"publisher","first-page":"2341","DOI":"10.1111\/ajt.16522","article-title":"Microbiota and immunoregulation: A focus on regulatory B lymphocytes and transplantation.","volume":"21","author":"Pacaud","year":"2021","journal-title":"Am. J. Transplant."},{"key":"B34","doi-asserted-by":"publisher","first-page":"1088","DOI":"10.1136\/gutjnl-2020-321173","article-title":"Bacteria-derived long chain fatty acid exhibits anti-inflammatory properties in colitis.","volume":"70","author":"Pujo","year":"2021","journal-title":"Gut"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.572912","article-title":"Antibiotics as major disruptors of gut microbiota.","volume":"10","author":"Ramirez","year":"2020","journal-title":"Front. Cell Infect. Microbiol."},{"key":"B36","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1016\/S0140-6736(03)14334-6","article-title":"Ultrasound in tropical and parasitic diseases.","volume":"362","author":"Richter","year":"2003","journal-title":"Lancet"},{"key":"B37","doi-asserted-by":"publisher","first-page":"7537","DOI":"10.1128\/AEM.01541-09","article-title":"Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities.","volume":"75","author":"Schloss","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-2739-2","article-title":"Investigations on the interplays between Schistosoma mansoni, praziquantel and the gut microbiome.","volume":"11","author":"Schneeberger","year":"2018","journal-title":"Parasit. Vectors"},{"key":"B39","doi-asserted-by":"crossref","DOI":"10.1186\/gb-2011-12-6-r60","article-title":"Metagenomic biomarker discovery and explanation.","volume":"12","author":"Segata","year":"2011","journal-title":"Genome Biol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1016\/S1473-3099(06)70521-7","article-title":"Schistosomiasis and water resources development: systematic review, meta-analysis, and estimates of people at risk.","volume":"6","author":"Steinmann","year":"2006","journal-title":"Lancet Infect. Dis."},{"key":"B41","doi-asserted-by":"publisher","first-page":"1859","DOI":"10.1017\/S0031182009991600","article-title":"Schistosomiasis and neglected tropical diseases: towards integrated and sustainable control and a word of caution.","volume":"136","author":"Utzinger","year":"2009","journal-title":"Parasitology"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009578","article-title":"Elimination of schistosomiasis in China: Current status and future prospects.","volume":"15","author":"Wang","year":"2021","journal-title":"PLoS Negl. Trop. Dis."},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2022.109118","article-title":"Metabolomics and microbiomes for discovering biomarkers of antituberculosis drugs-induced hepatotoxicity.","volume":"716","author":"Wu","year":"2022","journal-title":"Arch. Biochem. Biophys."},{"key":"B44","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1007\/s00436-014-4225-x","article-title":"Research and control of advanced schistosomiasis japonica in China.","volume":"114","author":"Wu","year":"2015","journal-title":"Parasitol. Res."},{"key":"B45","article-title":"Schistosomiasis situation changes after migration for 3 years in Qingtan town","author":"Yan","year":"2003","journal-title":"Chin. J. Schistosomiasis Control"},{"key":"B46","doi-asserted-by":"crossref","DOI":"10.1016\/j.metabol.2021.154712","article-title":"Role of the gut microbiota in type 2 diabetes and related diseases.","volume":"117","author":"Yang","year":"2021","journal-title":"Metabolism"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-12721-4","article-title":"Prevotella species in the human gut is primarily comprised of Prevotella copri, Prevotella stercorea and related lineages.","volume":"12","author":"Yeoh","year":"2022","journal-title":"Sci. Rep."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2023.1276404\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T08:04:47Z","timestamp":1696320287000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2023.1276404\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,3]]},"references-count":47,"alternative-id":["10.3389\/fmicb.2023.1276404"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2023.1276404","relation":{},"ISSN":["1664-302X"],"issn-type":[{"type":"electronic","value":"1664-302X"}],"subject":[],"published":{"date-parts":[[2023,10,3]]},"article-number":"1276404"}}