{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T15:13:15Z","timestamp":1789398795741,"version":"build-2803163510"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T00:00:00Z","timestamp":1698624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"Background<\/jats:title>Streptococcus agalactiae<\/jats:italic> or Group B Streptococcal<\/jats:italic> colonization of the gastrointestinal and genital tracts of pregnant women usually remains asymptomatic, even though it is the critical determinant of infection in neonates and young infants. It causes early and late onset of invasive Group B Streptococcus (GBS)<\/jats:italic> disease manifesting as septicemia, meningitis, and pneumonia. Now it is recognized as an important cause of maternal and neonatal morbidity and mortality in many parts of the world including Ethiopia, where the magnitude of the problem has been little studied. The aim of this study was to assess the prevalence of GBS<\/jats:italic> colonization and to identify associated risk factors and antimicrobial susceptibility patterns among pregnant women at selected health facilities of Wolaita Sodo Town, Southern Ethiopia.<\/jats:p><\/jats:sec>Methodology<\/jats:title>A health-facility-based cross-sectional study design was conducted at WSUCSH &amp; Wolaita Sodo Health Center from June to August, 2022. A total of 279 pregnant women who were in ANC follow-up at 35\u201337\u2009weeks of gestation were included. For GBS<\/jats:italic> isolation, recto-vaginal swabs were inoculated in 1\u2009mL Todd-Hewitt broth medium supplemented with 10\u2009\u03bcg\/mL colistin and 15\u2009\u03bcg\/mL nalidixic acid, followed by identification of isolates based on colonial morphology, gram stains, catalase reaction, and CAMP tests. Antimicrobial susceptibility testing was performed using a modified Kirby\u2013Bauer disc diffusion method. All collected data were entered in Epi info 4.6.0.2, then transferred and tabulated using SPSS version 20. Logistic regression analysis was used to see the association between variables. Finally, a p<\/jats:italic>-value &lt;0.05 was considered statistically significant.<\/jats:p><\/jats:sec>Results<\/jats:title>In the present study, 279 pregnant mothers, aged between 15 to 38\u2009years with a mean of 26.5\u2009\u00b1\u20094.5\u2009years, were included. Of all participants, the highest proportion (120) (43.01%) were housewives. The overall carriage rate of GBS<\/jats:italic> was 67 (24.0%). GBS<\/jats:italic> colonization showed a statistically significant association with college and above levels of maternal education [AOR\u2009=\u20096.610, 95% CI (1.724\u201325.349), p<\/jats:italic> =\u20090.01]. High susceptibility of GBS<\/jats:italic> isolate was seen with Penicillin G &amp; Chloramphenicol (92.5%), Ampicillin, Ceftriaxone (89.6%), Vancomycin (74.62%), and Erythromycin (77%). Relatively, GBS<\/jats:italic> showed high resistance to Tetracycline (88%).<\/jats:p><\/jats:sec>Conclusion and recommendation<\/jats:title>In this study, the overall prevalence of GBS<\/jats:italic> colonization was 24.0%. College and above educational level was statistically significant with GBS<\/jats:italic> colonization. This study aimed to draw attention to the management of Group B Streptococci<\/jats:italic> in pregnant women by making GBS<\/jats:italic> culture one of the routine diagnoses during ANC follow-up and to prevent infection with early detection.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2023.1277928","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T11:04:11Z","timestamp":1698663851000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Group B Streptococci recto-vaginal colonization, antimicrobial susceptibility pattern, and associated factors among pregnant women at selected health facilities of Wolaita Sodo Town, Southern Ethiopia"],"prefix":"10.3389","volume":"14","author":[{"given":"Abera","family":"Kumalo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biruk","family":"Gebre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimelis","family":"Shiferaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wokil","family":"Wolde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamirayehu","family":"Shonde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,10,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13104-015-1475-3","article-title":"Isolation and anti-microbial susceptibility pattern of group B Streptococcus among pregnant women attending antenatal clinics in Ayder referral hospital and Mekelle health center, Mekelle, Northern Ethiopia","volume":"8","author":"Alemseged","year":"2015","journal-title":"BMC Res. Notes"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"258","DOI":"10.14740\/jcgo341w","article-title":"Prevalence of maternal genital tract colonization by group B streptococcus from Western Province, Taif, Saudi Arabia","volume":"4","author":"Arain","year":"2015","journal-title":"J. Clin. Gynecol. Obstet."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1186\/s12884-018-1791-4","article-title":"Group B streptococci vaginal colonization and drug susceptibility pattern among pregnant women attending in selected public antenatal care centers in Addis Ababa, Ethiopia","volume":"18","author":"Assefa","year":"2018","journal-title":"BMC Pregnancy Childbirth"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1111\/j.1365-3016.1994.tb00449.x","article-title":"Maternal carriage and neonatal colonisation of group B streptococci in labour are uncommon in Turkey","volume":"8","author":"Ayata","year":"1994","journal-title":"Paediatr. Perinat. Epidemiol."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1093\/infdis\/173.2.365","article-title":"Invasive group B streptococcal disease: the emergence of serotype V","volume":"173","author":"Blumberg","year":"1996","journal-title":"J. Infect. Dis."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13104-015-1328-0","article-title":"Antibiotic resistance of Streptococcus agalactiae isolated from pregnant women in Garankuwa, South Africa","volume":"8","author":"Bolukaoto","year":"2015","journal-title":"BMC Res. Notes"},{"key":"ref7","article-title":"Prevention of perinatal group B streptococcal disease: revised recommendations from CDC","volume":"51","year":"2002","journal-title":"MMWR"},{"key":"ref8","first-page":"701","article-title":"Perinatal group B streptococcal disease after universal screening recommendations--United States, 2003\u20132005","volume":"56","year":"2007","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"ref9","volume-title":"Performance standards for antimicrobial susceptibility testing. 31st ed. CLSI supplement M100","year":"2021"},{"key":"ref10","first-page":"M100","article-title":"Performance standards for antimicrobial susceptibility testing: twenty-second informational supplement","volume":"32","author":"Cockerill","year":"2012","journal-title":"Clin. Lab. Stand. Inst."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1155\/2022\/8163396","article-title":"Vertical transmission, risk factors, and antimicrobial resistance patterns of group B Streptococcus among mothers and their neonates in southern Ethiopia","author":"Dadi","year":"2022","journal-title":"Can. J. Infect. Dis. Med. Microbiol."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1111\/j.1365-3156.2008.02018.x","article-title":"Low carriage rate of group B streptococcus in pregnant women in Maputo, Mozambique","volume":"13","author":"De Steenwinkel","year":"2008","journal-title":"Trop. Med. Int. Health"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/01443615.2020.1716313","article-title":"Group B Streptococcus colonisation and their antimicrobial susceptibility among pregnant women attending antenatal clinics in tertiary care hospitals in the Western Province of Sri Lanka","volume":"41","author":"Dilrukshi","year":"2021","journal-title":"J. Obstet. Gynaecol."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2334-10-285","article-title":"Comparison of different sampling techniques and of different culture methods for detection of group B streptococcus carriage in pregnant women","volume":"10","author":"El Aila","year":"2010","journal-title":"BMC Infect. Dis."},{"key":"ref15","first-page":"56","article-title":"Detection of genital colonization of group B streptococci during late pregnancy","volume":"23","author":"El-Kersh","year":"2002","journal-title":"Saudi Med. J."},{"key":"ref16","first-page":"30","article-title":"Colonization rate of group B Streptococcus (GBS) in pregnant women using GBS agar medium","volume":"25","author":"Fatemi","year":"2009","journal-title":"Acta Med. Iran."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"e0222910","DOI":"10.1371\/journal.pone.0222910","article-title":"Genetically distinct group B Streptococcus strains induce varying macrophage cytokine responses","volume":"14","author":"Flaherty","year":"2019","journal-title":"PLoS One"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1155\/S106474490000017X","article-title":"Is antenatal group B streptococcal carriage a predictor of adverse obstetric outcome?","volume":"8","author":"Garland","year":"2000","journal-title":"Infect. Dis. Obstet. Gynecol."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1186\/s12941-019-0303-3","article-title":"Streptococcus agalactiae from Ethiopian pregnant women; prevalence, associated factors and antimicrobial resistance: alarming for prophylaxis","volume":"18","author":"Gizachew","year":"2019","journal-title":"Ann. Clin. Microbiol. Antimicrob."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1186\/s12879-020-05035-1","article-title":"Prevalence, antimicrobial susceptibility patterns, serotypes and risk factors for group B streptococcus rectovaginal isolates among pregnant women at Kenyatta National Hospital, Kenya; a cross-sectional study","volume":"20","author":"Jisuvei","year":"2020","journal-title":"BMC Infect. Dis."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"101586","DOI":"10.1016\/j.bjid.2021.101586","article-title":"Capsular genotype distribution of group B streptococcus colonization among at-risk pregnant women in S\u00e3o Paulo, Brazil","volume":"25","author":"Kfouri","year":"2021","journal-title":"Braz. J. Infect. Dis."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"423","DOI":"10.5144\/0256-4947.2015.423","article-title":"Maternal colonization of group B streptococcus: prevalence, associated factors and antimicrobial resistance","volume":"35","author":"Khan","year":"2015","journal-title":"Ann. Saudi Med."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"1076","DOI":"10.1016\/S1473-3099(16)30055-X","article-title":"Prevalence of maternal colonisation with group B streptococcus: a systematic review and meta-analysis","volume":"16","author":"Kwatra","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1099\/00222615-42-3-161","article-title":"Comparison of streptococci of serological group B isolated from healthy carriers and active disease in Chile","volume":"42","author":"L\u00e4mmler","year":"1995","journal-title":"J. Med. Microbiol."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"5457","DOI":"10.2147\/IDR.S343429","article-title":"Group B streptococci vaginal-recto colonization, vertical transmission to newborns, antimicrobial susceptibility profile and associated factors in selected health facilities of Bahir Dar City","volume":"14","author":"Leykun","year":"2021","journal-title":"Infect. Drug Resist."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"335","DOI":"10.4236\/aim.2021.117026","article-title":"Vaginal colonization and antibiotic susceptibility pattern of group B Streptococcus isolated from pregnant women in Maternit\u00e9 de l\u2019H\u00f4pital Des Soeurs de Pauvres de Bergame de Kimbanseke, Kinshasa, Democratic Republic of Congo","volume":"11","author":"Liesse Iyamba","year":"2021","journal-title":"Adv. Microbiol."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"D31","DOI":"10.1016\/j.vaccine.2013.05.012","article-title":"Group B streptococcal epidemiology and vaccine needs in developed countries","volume":"31","author":"Melin","year":"2013","journal-title":"Vaccine"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"e2","DOI":"10.1590\/s1678-9946201860002","article-title":"Prevalence of Streptococcus agalactiae colonization in pregnant women from the 18 th health region of Paran\u00e1 state","volume":"60","author":"Melo","year":"2018","journal-title":"Rev. Inst. Med. Trop. S\u00e3o Paulo"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1186\/s13104-016-2158-4","article-title":"Prevalence and antimicrobial susceptibility pattern of anorectal and vaginal group B streptococci isolates among pregnant women in Jimma, Ethiopia","volume":"9","author":"Mengist","year":"2016","journal-title":"BMC Res. Notes"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1186\/s13104-017-2725-3","article-title":"Prevalence and drug susceptibility pattern of group B streptococci (GBS) among pregnant women attending antenatal care (ANC) in Nekemte referral hospital (NRH), Nekemte, Ethiopia","volume":"10","author":"Mengist","year":"2017","journal-title":"BMC Res. Notes"},{"key":"ref31","first-page":"36","article-title":"Prevalence of group B Streptococcus colonization among pregnant women attending antenatal clinic of Hawassa health center","volume":"38","author":"Mohammed","year":"2010","journal-title":"Ethiop. J. Health Dev."},{"key":"ref32","first-page":"36","article-title":"Prevalence of group B Streptococcus colonization among pregnant women attending antenatal clinic of Hawassa health center, Hawassa, Ethiopia","volume":"26","author":"Mohammed","year":"2012","journal-title":"Ethiop. J. Health Dev."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1136\/archdischild-2016-311419","article-title":"Challenges in reducing group B Streptococcus disease in African settings","volume":"102","author":"Nishihara","year":"2017","journal-title":"Arch. Dis. Child."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1007\/s10096-020-04011-6","article-title":"Persistence of group B Streptococcus vaginal colonization and prevalence of hypervirulent CC-17 clone correlate with the country of birth: a prospective 3-month follow-up cohort study","volume":"40","author":"Plainvert","year":"2021","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"794","DOI":"10.1128\/AAC.47.2.794-797.2003","article-title":"Genetic basis of antibiotic resistance in Streptococcus agalactiae strains isolated in a French hospital","volume":"47","author":"Poyart","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1515\/JPM.2009.020","article-title":"Group B Streptococcus colonization in pregnancy: prevalence and prevention strategies of neonatal sepsis","volume":"37","author":"Rausch","year":"2009","journal-title":"J. Perinat. Med."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.jinf.2007.11.007","article-title":"Streptococcus agalactiae in pregnant women: phenotypic and genotypic characters","volume":"56","author":"Savoia","year":"2008","journal-title":"J. Infect."},{"key":"ref38","first-page":"115","article-title":"Colonization of pregnant women and their newborn infants with group B streptococci in the Gondar College of Medical Sciences","volume":"27","author":"Schmidt","year":"1989","journal-title":"Ethiop. Med. J."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"104","DOI":"10.11648\/j.ajhr.20190706.12","article-title":"Prevalence of group b Streptococcus, its associated factors and antimicrobial susceptibility pattern among pregnant women attending antenatal Care at Arbaminch Hospital, South Ethiopia","volume":"7","author":"Shiferawu","year":"2019","journal-title":"Am. J. Health Res."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1099\/jmm.0.002865-0","article-title":"Group B streptococcus colonization of pregnant women and their children observed on obstetric and neonatal wards of the University Hospital in Krakow, Poland","volume":"58","author":"Strus","year":"2009","journal-title":"J. Med. Microbiol."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"832","DOI":"10.1046\/j.1469-0691.2003.00662.x","article-title":"Group B streptococcus colonization of Greek pregnant women and neonates: prevalence, risk factors and serotypes","volume":"9","author":"Tsolia","year":"2003","journal-title":"Clin. Microbiol. Infect."},{"key":"ref42","first-page":"414","article-title":"Change in prevalence of group B Streptococcus maternal colonisation in Hong Kong","volume":"15","author":"Tsui","year":"2009","journal-title":"Hong Kong Med. J."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1186\/s13756-021-00897-9","article-title":"Vaginal colonization with antimicrobial-resistant bacteria among women in labor in Central Uganda: prevalence and associated factors","volume":"10","author":"Tumuhamye","year":"2021","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"ref44","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1016\/j.ejogrb.2005.06.007","article-title":"Prevalence of colonisation with group B streptococci in pregnant women of a multi-ethnic population in the Netherlands","volume":"124","author":"Valkenburg-Van Den Berg","year":"2006","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref45","volume-title":"Prevention of perinatal group B streptococcal disease: Revised guidelines from CDC, 2010","author":"Verani","year":"2010"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1742-4755-11-80","article-title":"The prevalence of group B streptococus recto-vaginal colonization and antimicrobial susceptibility pattern in pregnant mothers at two hospitals of Addis Ababa, Ethiopia","volume":"11","author":"Woldu","year":"2014","journal-title":"Reprod. Health"},{"key":"ref47","volume-title":"Wolaita Sodo University comprehensive specialized hospital healthcare quality Progress","year":"2022"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"731","DOI":"10.18502\/ijrm.v16i12.3679","article-title":"Prevalence of group B streptococcus colonization in Iranian pregnant women: a systematic review and meta-analysis","volume":"16","author":"YektaKooshali","year":"2018","journal-title":"Int. J. Reprod. 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