{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T16:03:47Z","timestamp":1782921827958,"version":"3.54.5"},"reference-count":69,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T00:00:00Z","timestamp":1697673600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Acetogenic bacteria can utilize C1 compounds, such as carbon monoxide (CO), formate, and methanol, via the Wood-Ljungdahl pathway (WLP) to produce biofuels and biochemicals. Two novel acetogenic bacteria of the family <jats:italic>Eubacteriaceae<\/jats:italic> ES2 and ES3 were isolated from Eulsukdo, a delta island in South Korea. We conducted whole genome sequencing of the ES strains and comparative genome analysis on the core clusters of WLP with <jats:italic>Acetobacterium woodii<\/jats:italic> DSM1030<jats:sup>T<\/jats:sup> and <jats:italic>Eubacterium limosum<\/jats:italic> ATCC8486<jats:sup>T<\/jats:sup>. The methyl-branch cluster included a formate transporter and duplicates or triplicates copies of the <jats:italic>fhs<\/jats:italic> gene, which encodes formyl-tetrahydrofolate synthetase. The formate dehydrogenase cluster did not include the hydrogenase gene, which might be replaced by a functional complex with a separate electron bifurcating hydrogenase (HytABCDE). Additionally, duplicated copies of the <jats:italic>acsB<\/jats:italic> gene, encoding acetyl-CoA synthase, are located within or close to the carbonyl-branch cluster. The serum bottle culture showed that ES strains can utilize a diverse range of C1 compounds, including CO, formate, and methanol, as well as CO<jats:sub>2<\/jats:sub>. Notably, ES2 exhibited remarkable resistance to high concentrations of C1 substrates, such as 100% CO (200\u2009kPa), 700\u2009mM formate, and 500\u2009mM methanol. Moreover, ES2 demonstrated remarkable growth rates under 50% CO (0.45\u2009h<jats:sup>\u22121<\/jats:sup>) and 200\u2009mM formate (0.34\u2009h<jats:sup>\u22121<\/jats:sup>). These growth rates are comparable to or surpassing those previously reported in other acetogenic bacteria. Our study introduces novel acetogenic ES strains and describes their genetic and physiological characteristics, which can be utilized in C1-based biomanufacturing.<\/jats:p>","DOI":"10.3389\/fmicb.2023.1279544","type":"journal-article","created":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T13:34:41Z","timestamp":1697722481000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Genomic potential and physiological characteristics of C1 metabolism in novel acetogenic bacteria"],"prefix":"10.3389","volume":"14","author":[{"given":"Jihyun","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi-Jeong","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joungmin","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo Jae","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Kyu","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun Sook","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Gyun","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung-Hyun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kae Kyoung","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun Jae","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,10,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1007\/BF00303591","article-title":"Clostridium autoethanogenum, sp. nov., an anaerobic bacterium that produces ethanol from carbon monoxide","volume":"161","author":"Abrini","year":"1994","journal-title":"Arch. Microbiol."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"867","DOI":"10.1128\/jb.116.2.867-873.1973","article-title":"Formate dehydrogenase of Clostridium thermoaceticum: incorporation of selenium-75, and the effects of selenite, molybdate, and tungstate on the enzyme","volume":"116","author":"Andreesen","year":"1973","journal-title":"J. Bacteriol."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"2500","DOI":"10.1038\/s41467-020-16366-7","article-title":"Precise phylogenetic analysis of microbial isolates and genomes from metagenomes using PhyloPhlAn 3.0","volume":"11","author":"Asnicar","year":"2020","journal-title":"Nat. Commun."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"7455","DOI":"10.1021\/bi992583z","article-title":"Reversible carbon monoxide binding and inhibition at the active site of the Fe-only hydrogenase","volume":"39","author":"Bennett","year":"2000","journal-title":"Biochemistry"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"5949","DOI":"10.1128\/AEM.01772-15","article-title":"CO metabolism in the acetogen Acetobacterium woodii","volume":"81","author":"Bertsch","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1186\/s13068-022-02147-5","article-title":"Formate-driven H2 production by whole cells of Thermoanaerobacter kivui","volume":"15","author":"Burger","year":"2022","journal-title":"Biotechnol. Biofuels Bioproducts"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1039\/C6EE02494G","article-title":"The hydrogen dependent CO2 reductase: the first completely CO tolerant FeFe-hydrogenase","volume":"10","author":"Ceccaldi","year":"2017","journal-title":"Energy Environ. Sci."},{"key":"ref25","first-page":"134","article-title":"CO fermentation of Eubacterium limosum KIST612","volume-title":"Journal of Microbiology and Biotechnology","author":"Chang","year":"1998"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1016\/j.energy.2019.03.161","article-title":"A novel path for carbon-rich resource utilization with lower emission and higher efficiency: an integrated process of coal gasification and coking to methanol production","volume":"177","author":"Chen","year":"2019","journal-title":"Energy"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1038\/s41929-019-0272-0","article-title":"Making quantitative sense of electromicrobial production","volume":"2","author":"Claassens","year":"2019","journal-title":"Nature Catalysis"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.copbio.2019.10.002","article-title":"Renewable methanol and formate as microbial feedstocks","volume":"62","author":"Cotton","year":"2020","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1016\/0006-291X(87)90384-6","article-title":"Electron paramagnetic resonance studies of the tungsten-containing formate dehydrogenase from Clostridium thermoaceticum","volume":"149","author":"Deaton","year":"1987","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1007\/0-387-30742-7_13","article-title":"Acetogenic prokaryotes","volume":"2","author":"Denned","year":"2006","journal-title":"Prokaryotes"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"4505","DOI":"10.1111\/1462-2920.15643","article-title":"Biochemistry of methanol-dependent acetogenesis in Eubacterium callanderi KIST612","volume":"23","author":"Dietrich","year":"2021","journal-title":"Environ. Microbiol."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1196\/annals.1419.016","article-title":"Old acetogens, new light","volume":"1125","author":"Drake","year":"2008","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"e938","DOI":"10.1002\/mbo3.938","article-title":"Insights into the genome structure of four acetogenic bacteria with specific reference to the Wood-Ljungdahl pathway","volume":"8","author":"Esposito","year":"2019","journal-title":"Microbiology"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1007\/BF01734359","article-title":"Evolutionary trees from DNA sequences: a maximum likelihood approach","volume":"17","author":"Felsenstein","year":"1981","journal-title":"J. Mol. Evol."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"1348","DOI":"10.1111\/1462-2920.15434","article-title":"Anaerobic microbial methanol conversion in marine sediments","volume":"23","author":"Fischer","year":"2021","journal-title":"Environ. Microbiol."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1093\/sysbio\/20.4.406","article-title":"Toward defining the course of evolution: minimum change for a specific tree topology","volume":"20","author":"Fitch","year":"1971","journal-title":"Syst. Biol."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1186\/s13068-021-01966-2","article-title":"Production of the biocommodities butanol and acetone from methanol with fluorescent FAST-tagged proteins using metabolically engineered strains of Eubacterium limosum","volume":"14","author":"Flaiz","year":"2021","journal-title":"Biotechnol. Biofuels"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1111\/1751-7915.12149","article-title":"Kinetic and stoichiometric characterization of organoautotrophic growth of Ralstonia eutropha on formic acid in fed-batch and continuous cultures","volume":"8","author":"Grunwald","year":"2015","journal-title":"Microb. Biotechnol."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"1090","DOI":"10.1016\/j.bbapap.2014.12.006","article-title":"Assembly and catalysis of molybdenum or tungsten-containing formate dehydrogenases from bacteria","volume":"1854","author":"Hartmann","year":"2015","journal-title":"Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/B978-1-4832-3211-9.50009-7","article-title":"Evolution of protein molecules","volume-title":"Mammalian protein metabolism","author":"Jukes","year":"1969"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"402","DOI":"10.3389\/fmicb.2020.00402","article-title":"Adaptive laboratory evolution of Eubacterium limosum ATCC 8486 on carbon monoxide","volume":"11","author":"Kang","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT multiple sequence alignment software version 7: improvements in performance and usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"124521","DOI":"10.1016\/j.biortech.2020.124521","article-title":"Methanol supply speeds up synthesis gas fermentation by methylotrophic-acetogenic bacterium, Eubacterium limosum KIST612","volume":"321","author":"Kim","year":"2021","journal-title":"Bioresour. Technol."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1016\/j.copbio.2011.01.005","article-title":"Fermentative production of ethanol from carbon monoxide","volume":"22","author":"K\u00f6pke","year":"2011","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1186\/s13068-018-1082-3","article-title":"Efficient whole cell biocatalyst for formate-based hydrogen production","volume":"11","author":"Kottenhahn","year":"2018","journal-title":"Biotechnol. Biofuels"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"fuaa040","DOI":"10.1093\/femsre\/fuaa040","article-title":"Methanol and methyl group conversion in acetogenic bacteria: biochemistry, physiology and application","volume":"45","author":"Kremp","year":"2021","journal-title":"FEMS Microbiol. Rev."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"4369","DOI":"10.1111\/1462-2920.14356","article-title":"Methanol metabolism in the acetogenic bacterium Acetobacterium woodii","volume":"20","author":"Kremp","year":"2018","journal-title":"Environ. Microbiol."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"865168","DOI":"10.3389\/fmicb.2022.865168","article-title":"Engineering acetogenic bacteria for efficient one-carbon utilization","volume":"13","author":"Lee","year":"2022","journal-title":"Front. Microbiol."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13068-019-1570-0","article-title":"Domestication of the novel alcohologenic acetogen Clostridium sp. AWRP: from isolation to characterization for syngas fermentation","volume":"12","author":"Lee","year":"2019","journal-title":"Biotechnol. Biofuels"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"2675","DOI":"10.1128\/jb.01814-08","article-title":"A molybdopterin oxidoreductase is involved in H2 oxidation in Desulfovibrio desulfuricans G20","volume":"191","author":"Li","year":"2009","journal-title":"J. Bacteriol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"694","DOI":"10.3389\/fmicb.2016.00694","article-title":"Gas fermentation\u2014a flexible platform for commercial scale production of low-carbon-fuels and chemicals from waste and renewable feedstocks","volume":"7","author":"Liew","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1128\/jb.159.1.375-380.1984","article-title":"Formate dehydrogenase of Clostridium pasteurianum","volume":"159","author":"Liu","year":"1984","journal-title":"J. Bacteriol."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"128259","DOI":"10.1016\/j.biortech.2022.128259","article-title":"C1-based biomanufacturing: advances, challenges and perspectives","volume":"367","author":"Lv","year":"2022","journal-title":"Bioresour. Technol."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"3020","DOI":"10.1039\/C5GC02708J","article-title":"Low carbon fuels and commodity chemicals from waste gases\u2013systematic approach to understand energy metabolism in a model acetogen","volume":"18","author":"Marcellin","year":"2016","journal-title":"Green Chem."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2105-14-60","article-title":"Genome sequence-based species delimitation with confidence intervals and improved distance functions","volume":"14","author":"Meier-Kolthoff","year":"2013","journal-title":"BMC Bioinformat"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"4214","DOI":"10.1111\/1462-2920.15598","article-title":"Formate metabolism in the acetogenic bacterium Acetobacterium woodii","volume":"23","author":"Moon","year":"2021","journal-title":"Environ. Microbiol."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"571736","DOI":"10.3389\/fmicb.2020.571736","article-title":"Homoacetogenic conversion of mannitol by the thermophilic acetogenic bacterium Thermoanaerobacter kivui requires external CO2","volume":"11","author":"Moon","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"6345","DOI":"10.1128\/AEM.69.11.6345-6353.2003","article-title":"Energy conservation in acetogenic bacteria","volume":"69","author":"M\u00fcller","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"1344","DOI":"10.1016\/j.tibtech.2019.05.008","article-title":"New horizons in acetogenic conversion of one-carbon substrates and biological hydrogen storage","volume":"37","author":"M\u00fcller","year":"2019","journal-title":"Trends Biotechnol."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1007\/BF00266289","article-title":"Effects of various organic acid supplements on growth rates of Eubacterium limosum B2 on methanol","volume":"24","author":"Pacaud","year":"1986","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"1641","DOI":"10.1093\/molbev\/msp077","article-title":"FastTree: computing large minimum evolution trees with profiles instead of a distance matrix","volume":"26","author":"Price","year":"2009","journal-title":"Mol. Biol. Evol."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"2210","DOI":"10.1128\/jb.01688-14","article-title":"A proposed genus boundary for the prokaryotes based on genomic insights","volume":"196","author":"Qin","year":"2014","journal-title":"J. Bacteriol."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"e00277","DOI":"10.1128\/msystems.00277-20","article-title":"Defining genomic and predicted metabolic features of the Acetobacterium genus","volume":"5","author":"Ross","year":"2020","journal-title":"Msystems"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1093\/oxfordjournals.molbev.a040454","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1111\/j.1574-6968.2007.00737.x","article-title":"Cold-active acetogenic bacteria from surficial sediments of perennially ice-covered Lake Fryxell, Antarctica","volume":"272","author":"Sattley","year":"2007","journal-title":"FEMS Microbiol. Lett."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"1382","DOI":"10.1126\/science.1244758","article-title":"Direct and reversible hydrogenation of CO2 to formate by a bacterial carbon dioxide reductase","volume":"342","author":"Schuchmann","year":"2013","journal-title":"Science"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"809","DOI":"10.1038\/nrmicro3365","article-title":"Autotrophy at the thermodynamic limit of life: a model for energy conservation in acetogenic bacteria","volume":"12","author":"Schuchmann","year":"2014","journal-title":"Nat. Rev. Microbiol."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"1311","DOI":"10.1111\/febs.13670","article-title":"A bacterial hydrogen-dependent CO2 reductase forms filamentous structures","volume":"283","author":"Schuchmann","year":"2016","journal-title":"FEBS J."},{"key":"ref53","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1186\/s13068-018-1236-3","article-title":"Hydrogenation of CO2 at ambient pressure catalyzed by a highly active thermostable biocatalyst","volume":"11","author":"Schwarz","year":"2018","journal-title":"Biotechnol. Biofuels"},{"key":"ref54","doi-asserted-by":"publisher","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"ref55","doi-asserted-by":"publisher","first-page":"13694","DOI":"10.1038\/s41598-017-14123-3","article-title":"Determination of the genome and primary transcriptome of syngas fermenting Eubacterium limosum ATCC 8486","volume":"7","author":"Song","year":"2017","journal-title":"Sci. Rep."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"1312","DOI":"10.1093\/bioinformatics\/btu033","article-title":"RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies","volume":"30","author":"Stamatakis","year":"2014","journal-title":"Bioinformatics"},{"key":"ref57","doi-asserted-by":"publisher","first-page":"11030","DOI":"10.1073\/pnas.040420610","article-title":"Prospects for inferring very large phylogenies by using the neighbor-joining method","volume":"101","author":"Tamura","year":"2004","journal-title":"Proc. Natl. Acad. Sci."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1099\/00207713-43-2-232","article-title":"Clostridium ljungdahlii sp. nov., an acetogenic species in clostridial rRNA homology group I","volume":"43","author":"Tanner","year":"1993","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/srep16168","article-title":"Adaptation of the autotrophic acetogen Sporomusa ovata to methanol accelerates the conversion of CO2 to organic products","volume":"5","author":"Tremblay","year":"2015","journal-title":"Sci. Rep."},{"key":"ref60","volume-title":"Graph clustering by flow simulation. Doctoral dissertation","author":"Van Dongen","year":"2000"},{"key":"ref61","doi-asserted-by":"publisher","first-page":"4373","DOI":"10.1128\/jb.00678-13","article-title":"NADP-specific electron-bifurcating [FeFe]-hydrogenase in a functional complex with formate dehydrogenase in Clostridium autoethanogenum grown on CO","volume":"195","author":"Wang","year":"2013","journal-title":"J. Bacteriol."},{"key":"ref62","doi-asserted-by":"publisher","first-page":"2312","DOI":"10.1128\/AEM.00122-16","article-title":"CO metabolism in the thermophilic acetogen Thermoanaerobacter kivui","volume":"82","author":"Weghoff","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"ref63","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-3-319-50430-8_4","article-title":"Synthesis of acetyl-CoA from carbon dioxide in acetogenic bacteria","volume-title":"Biogenesis of fatty acids, lipids and membranes, handbook of hydrocarbon and lipid microbiology","author":"Wiechmann","year":"2019"},{"key":"ref64","doi-asserted-by":"publisher","first-page":"720","DOI":"10.1016\/j.rser.2019.06.032","article-title":"Carbon dioxide management via exergy-based sustainability assessment: carbon capture and storage versus conversion to methanol","volume":"112","author":"Wiesberg","year":"2019","journal-title":"Renew. Sust. Energ. Rev."},{"key":"ref65","doi-asserted-by":"publisher","DOI":"10.1101\/2022.11.02.514939","article-title":"Characterisation of acetogen formatotrophic potential using E. limosum","author":"Wood","year":"2022","journal-title":"bio Rxiv"},{"key":"ref66","doi-asserted-by":"publisher","first-page":"1826","DOI":"10.1016\/S0021-9258(18)33062-X","article-title":"Purification and properties of NADP-dependent formate dehydrogenase from Clostridium thermoaceticum, a tungsten-selenium-iron protein","volume":"258","author":"Yamamoto","year":"1983","journal-title":"J. Biol. Chem."},{"key":"ref67","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1016\/j.biortech.2014.11.022","article-title":"Microbial synthesis gas utilization and ways to resolve kinetic and mass-transfer limitations","volume":"177","author":"Yasin","year":"2015","journal-title":"Bioresour. Technol."},{"key":"ref68","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.cbpa.2016.07.005","article-title":"The formate bio-economy","volume":"35","author":"Yishai","year":"2016","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref69","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1007\/s10482-017-0844-4","article-title":"A large-scale evaluation of algorithms to calculate average nucleotide identity","volume":"110","author":"Yoon","year":"2017","journal-title":"Antonie Van Leeuwenhoek"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2023.1279544\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T13:34:47Z","timestamp":1697722487000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2023.1279544\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,19]]},"references-count":69,"alternative-id":["10.3389\/fmicb.2023.1279544"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2023.1279544","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,19]]},"article-number":"1279544"}}