{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:13:43Z","timestamp":1790784823728,"version":"4.1.0"},"reference-count":54,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T00:00:00Z","timestamp":1704931200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p><jats:italic>Campylobacter concisus<\/jats:italic> is an oral bacterium. Recent studies suggest that <jats:italic>C. concisus<\/jats:italic> may be involved in human gastric diseases. The mechanisms, however, by which <jats:italic>C. concisus<\/jats:italic> causes human gastric diseases have not been investigated. Here we examined the gastric epithelial pathogenicity of <jats:italic>C. concisus<\/jats:italic> using a cell culture model. Six <jats:italic>C. concisus<\/jats:italic> strains and the human gastric epithelial cell line AGS cells were used. IL-8 produced by AGS cells after incubation with <jats:italic>C. concisus<\/jats:italic> was measured using enzyme-linked immunosorbent assay (ELISA), and AGS cell apoptosis was determined by caspase 3\/7 activities. The effects of <jats:italic>C. concisus<\/jats:italic> on actin arrangement in AGS cells was determined using fluorescence staining. The effects of <jats:italic>C. concisus<\/jats:italic> on global gene expression in AGS cells was determined by transcriptomic analysis and quantitative real-time PCR (qRT-PCR). The role of the upregulated <jats:italic>CYP1A1<\/jats:italic> gene in gastric cancer survival was assessed using the Kaplan-Meier method. <jats:italic>C. concisus<\/jats:italic> induced production of IL-8 by AGS cells with strain variation. Significantly increased caspase 3\/7 activities were observed in AGS cells incubated with <jats:italic>C. concisus<\/jats:italic> strains when compared to AGS cells without bacteria. <jats:italic>C. concisus<\/jats:italic> induced actin re-arrangement in AGS cells. <jats:italic>C. concisus<\/jats:italic> upregulated 30 genes in AGS cells and the upregulation of <jats:italic>CYP1A1<\/jats:italic> gene was confirmed by qRT-PCR. The Kaplan-Meier analysis showed that upregulation of <jats:italic>CYP1A1<\/jats:italic> gene is associated with worse survival in gastric cancer patients. Our findings suggest that <jats:italic>C. concisus<\/jats:italic> may play a role in gastric inflammation and the progression of gastric cancer. Further investigation in clinical studies is warranted.<\/jats:p>","DOI":"10.3389\/fmicb.2023.1289549","type":"journal-article","created":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T04:17:35Z","timestamp":1704946655000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Investigation of Campylobacter concisus gastric epithelial pathogenicity using AGS cells"],"prefix":"10.3389","volume":"14","author":[{"given":"Christopher Yau Man","family":"Luk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seul A.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Naidovski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfred Chin Yen","family":"Tay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Riordan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,1,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13099-020-00397-y","article-title":"High genetic diversity in Campylobacter concisus isolates from patients with microscopic colitis.","volume":"13","author":"Aagaard","year":"2021","journal-title":"Gut Pathog."},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-9-187","article-title":"Cytochrome P450 CYP1A1: Wider roles in cancer progression and prevention.","volume":"9","author":"Androutsopoulos","year":"2009","journal-title":"BMC Cancer"},{"key":"B3","doi-asserted-by":"publisher","first-page":"506","DOI":"10.1111\/j.1523-5378.2008.00646.x","article-title":"H. pylori-induced apoptosis in human gastric cancer cells mediated via the release of apoptosis-inducing factor from mitochondria.","volume":"13","author":"Ashktorab","year":"2008","journal-title":"Helicobacter"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01475-23","article-title":"The Campylobacter concisus BisA protein plays a dual role: Oxide-dependent anaerobic respiration and periplasmic methionine sulfoxide repair.","volume":"14","author":"Benoit","year":"2023","journal-title":"mBio"},{"key":"B5","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for Illumina sequence data.","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"B6","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1038\/s41588-020-0669-3","article-title":"Guidelines for human gene nomenclature.","volume":"52","author":"Bruford","year":"2020","journal-title":"Nat. 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