{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T11:42:44Z","timestamp":1786966964155,"version":"build-2736575974"},"reference-count":102,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T00:00:00Z","timestamp":1700006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Bartonelloses are neglected emerging infectious diseases caused by facultatively intracellular bacteria transmitted between vertebrate hosts by various arthropod vectors. The highest diversity of <jats:italic>Bartonella<\/jats:italic> species has been identified in rodents. Within this study we focused on the edible dormouse (<jats:italic>Glis glis<\/jats:italic>), a rodent with unique life-history traits that often enters households and whose possible role in the epidemiology of <jats:italic>Bartonella<\/jats:italic> infections had been previously unknown. We identified and cultivated two distinct <jats:italic>Bartonella<\/jats:italic> sub(species) significantly diverging from previously described species, which were characterized using growth characteristics, biochemical tests, and various molecular techniques including also proteomics. Two novel (sub)species were described: <jats:italic>Bartonella grahamii<\/jats:italic> subsp. <jats:italic>shimonis<\/jats:italic> subsp. nov. and <jats:italic>Bartonella gliris<\/jats:italic> sp. nov. We sequenced two individual strains per each described (sub)species. During exploratory genomic analyses comparing two genotypes ultimately belonging to the same species, both factually and most importantly even spatiotemporally, we noticed unexpectedly significant structural variation between them. We found that most of the detected structural variants could be explained either by prophage excision or integration. Based on a detailed study of one such event, we argue that prophage deletion represents the most probable explanation of the observed phenomena. Moreover, in one strain of <jats:italic>Bartonella grahamii<\/jats:italic> subsp. <jats:italic>shimonis<\/jats:italic> subsp. nov. we identified a deletion related to <jats:italic>Bartonella<\/jats:italic> Adhesin A, a major pathogenicity factor that modulates bacteria-host interactions. Altogether, our results suggest that even a limited number of passages induced sufficient selective pressure to promote significant changes at the level of the genome.<\/jats:p>","DOI":"10.3389\/fmicb.2023.1289671","type":"journal-article","created":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T09:39:21Z","timestamp":1700041161000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): hints of cultivation stress-induced genomic changes"],"prefix":"10.3389","volume":"14","author":[{"given":"Old\u0159ich","family":"Barto\u0161","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u011bla","family":"Klime\u0161ov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Volfov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Chmel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u0159\u00ed","family":"Dresler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Pajer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hana","family":"Kab\u00ed\u010dkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Adam\u00edk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Modr\u00fd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena Myslivcov\u00e1","family":"Fu\u010d\u00edkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Vot\u00fdpka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,11,15]]},"reference":[{"key":"ref1","unstructured":"AmoriG.\n            HuttererR.\n            Kry\u0161tufekB.\n            YigitN.\n            MitsainasG.\n            Mu\u00f1ozL.\n          2021"},{"key":"ref2","unstructured":"AndrewsS.\n          2010"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"2032","DOI":"10.3389\/fmicb.2018.02032","article-title":"Characterization of a prophage-free derivative strain of Lactococcus lactis ssp. 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