{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T20:39:36Z","timestamp":1789331976167,"version":"build-2803163510"},"reference-count":97,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,11,29]],"date-time":"2023-11-29T00:00:00Z","timestamp":1701216000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Urinary tract infections (UTI), among which the main etiological factor is uropathogenic\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      (UPEC,\n                      <jats:italic>E. coli<\/jats:italic>\n                      ), remain an important issue for clinicians. The aim of the study was to demonstrate clear differences in the pathogenic properties of urine-derived\n                      <jats:italic>E. coli<\/jats:italic>\n                      compared to other extraintestinal\n                      <jats:italic>E. coli<\/jats:italic>\n                      clinical isolates (derived from: blood, lower respiratory tracts, sputum, reproductive tract, body fluids, perianal pus, other pus, wound, postoperative wound and other sources).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The collection of 784\n                      <jats:italic>E. coli<\/jats:italic>\n                      isolates was collected from various materials of hospitalized patients. They were analyzed in terms of virulence-associated genes (\n                      <jats:italic>papC, sfaD\/sfaE, cnf1, usp., fimG\/H, hlyA<\/jats:italic>\n                      ), belonging to phylogenetic groups and the presence of CRISPR-\n                      <jats:italic>Cas<\/jats:italic>\n                      regions using PCR. In addition, the epidemiological data and the antibiotic resistance profiles provided by the hospital\u2019s microbiology department were included for statistical analyses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Urine-derived\n                      <jats:italic>E. coli<\/jats:italic>\n                      showed significantly greater virulence potential compared to other isolates, but they were generally unremarkable in terms of drug resistance. The isolates most often belonged to phylogenetic group B2. Drug resistance was negatively correlated with CRISPR 2 presence and high average virulence score, but positively correlated with CRISPR 4 presence. To the best of our knowledge, we are the first to report significant differences in sputum-derived isolates\u2014they revealed the lowest virulence potential and, at the same time, the highest drug resistance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      In conclusion, we demonstrated significant differences of urinary-derived\n                      <jats:italic>E. coli<\/jats:italic>\n                      compared to other clinical\n                      <jats:italic>E. coli<\/jats:italic>\n                      isolates. We would like to suggest excluding penicillins from use in\n                      <jats:italic>E. coli<\/jats:italic>\n                      infection at this time and monitoring strains with a high pathogenicity potential.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2023.1289683","type":"journal-article","created":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T23:54:32Z","timestamp":1701215672000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Comparative study of virulence potential, phylogenetic origin, CRISPR-Cas regions and drug resistance of Escherichia coli isolates from urine and other clinical materials"],"prefix":"10.3389","volume":"14","author":[{"given":"Anna","family":"Dziuba","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylwia","family":"Dzier\u017cak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Sodo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Wawszczak-Kasza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Zegad\u0142o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakub","family":"Bia\u0142ek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Zych","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wojciech","family":"Kiebzak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaros\u0142aw","family":"Matykiewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanis\u0142aw","family":"G\u0142uszek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wioletta","family":"Adamus-Bia\u0142ek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,11,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1080\/21505594.2019.1596507","article-title":"Ciprofloxacin, amoxicillin, and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic Escherichia coli strains","volume":"10","author":"Adamus-Bia\u0142ek","year":"2019","journal-title":"Virulence"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"3937","DOI":"10.1128\/JCM.01036-09","article-title":"(CGG)4-based PCR as a novel tool for discrimination of uropathogenic Escherichia coli strains: comparison with enterobacterial repetitive intergenic consensus-PCR","volume":"47","author":"Adamus-Bialek","year":"2009","journal-title":"J. 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