{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T16:59:23Z","timestamp":1778691563768,"version":"3.51.4"},"reference-count":62,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,12,4]],"date-time":"2023-12-04T00:00:00Z","timestamp":1701648000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Microorganisms are important sources of lipolytic enzymes with characteristics for wide promising usages in the specific industrial biotechnology. The cellulolytic myxobacterium <jats:italic>Sorangium cellulosum<\/jats:italic> is rich of lipolytic enzymes in the genome, but little has been investigated. Here, we discerned 406 potential lipolytic enzymes in 13 sequenced <jats:italic>S. cellulosum<\/jats:italic> genomes. These lipolytic enzymes belonged to 12 families, and most are novel with low identities (14\u201337%) to those reported. We characterized a new carboxylesterase, LipB, from the alkaline-adaptive So0157-2. This enzyme, belonging to family VIII, hydrolyzed glyceryl tributyrate and <jats:italic>p<\/jats:italic>-nitrophenyl esters with short chain fatty acids (\u2264C12), and exhibited the highest activity against <jats:italic>p<\/jats:italic>-nitrophenyl butyrate. It retained over 50% of the activities in a broad temperature range (from 20\u00b0C to 60\u00b0C), alkaline conditions (pH 8.0\u20139.5), and the enzymatic activity was stable with methanol, ethanol and isopropanol, and stimulated significantly in the presence of 5\u2009mM Ni<jats:sup>2+<\/jats:sup>. LipB also exhibited <jats:italic>\u03b2<\/jats:italic>-lactamase activity on nitrocefin, but not ampicillin, cefotaxime and imipenem. The bioinformatic analysis and specific enzymatic characteristics indicate that <jats:italic>S. cellulosum<\/jats:italic> is a promising resource to explore lipolytic enzymes for industrial adaptations.<\/jats:p>","DOI":"10.3389\/fmicb.2023.1304233","type":"journal-article","created":{"date-parts":[[2023,12,4]],"date-time":"2023-12-04T04:36:14Z","timestamp":1701664574000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Genome-wide analysis of lipolytic enzymes and characterization of a high-tolerant carboxylesterase from Sorangium cellulosum"],"prefix":"10.3389","volume":"14","author":[{"given":"Shu-Fei","family":"Yuan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin-Jing","family":"Yue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Feng","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue-Zhong","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2023,12,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"2871","DOI":"10.1007\/s00253-020-10429-x","article-title":"Current prospective in using cold-active enzymes as eco-friendly detergent additive","volume":"104","author":"Al-Ghanayem","year":"2020","journal-title":"Appl. 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