{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:56:00Z","timestamp":1777596960997,"version":"3.51.4"},"reference-count":68,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T00:00:00Z","timestamp":1704326400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p><jats:italic>Delftia tsuruhatensis<\/jats:italic> is a gram-negative, aerobic bacterium mostly known as an organic pollutant degrading and growth-promoting microorganism. However, it recently emerged as an opportunistic human pathogen. To date, the source of <jats:italic>D. tsuruhatensis<\/jats:italic> infection is not clear. The majority of studies of <jats:italic>D. tsuruhatensis<\/jats:italic> have focused on environmental or clinical strains, while investigations of <jats:italic>D. tsuruhatensis<\/jats:italic> strains isolated from food sources are limited. In the present study, we report the case of <jats:italic>D. tsuruhatensis<\/jats:italic> isolation from raw bovine milk. Classical bacteriology approaches, as well as next-generation sequencing and comparative genomics, were used to characterize the features of the <jats:italic>D. tsuruhatensis<\/jats:italic> MR-6\/3H strain. The MR-6\/3H strain was resistant to 19 antimicrobials among 23 tested, including all aminoglycosides, phenicol, trimethoprim-sulfamethoxazole, and almost all \u03b2-lactams. Phylogenetically, the MR-6\/3H was close to clinical origin strains, including those previously isolated in Russia. Comparative genomics revealed the presence of putative antimicrobial resistance genes in the MR-6\/3H isolate, mostly associated with efflux systems. Notably, genus-specific OXA-926-like \u03b2-lactamase was also detected. In all, 27 putative virulence factors were predicted, the majority of which were associated with motility, adherence, stress survival, siderophore synthesis, and immunomodulation. In the MR-6\/3H genome, the five prophage regions were identified, including two with intact levels. Integrons and CRISPR-Cas systems were not detected in the MR-6\/3H isolate. Thus, our findings suggest that raw milk can be the potential source of and transmission route for the dissemination of multidrug-resistant <jats:italic>D. tsuruhatensis<\/jats:italic>.<\/jats:p>","DOI":"10.3389\/fmicb.2023.1321122","type":"journal-article","created":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T04:58:40Z","timestamp":1704344320000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Genomic analysis of multidrug-resistant Delftia tsuruhatensis isolated from raw bovine milk"],"prefix":"10.3389","volume":"14","author":[{"given":"Pavel A.","family":"Andriyanov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daria D.","family":"Kashina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alena N.","family":"Menshikova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2024,1,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"W345","DOI":"10.1093\/NAR\/GKAC247","article-title":"The galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2022 update","volume":"50","author":"Afgan","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"2237","DOI":"10.1167\/IOVS.08-2785","article-title":"The role of twitching motility in Pseudomonas aeruginosa exit from and translocation of corneal epithelial cells","volume":"50","author":"Alarcon","year":"2009","journal-title":"Invest. 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